Development and preclinical evaluation of virus-like particle vaccine against COVID-19 infection

Date

2021-09-01

Author

YILMAZ, İSMAİL CEM
İpekoğlu, Emre Mert
Bulbul, Artun
Turay, Nilsu
Yildirim, Muzaffer
Evcili, Irem
Yilmaz, Naz Surucu
Guvencli, Nese
Aydin, Yagmur
Gungor, Bilgi
Saraydar, Berfu
Bartan, Asli Gulce
Ibibik, Bilgehan
Bildik, Tugce
Baydemir, Ilayda
Sanli, Hatice Asena
Kayaoğlu, Başak
Ceylan, Yasemin
Yildirim, Tugce
Abras, Irem
Ayanoğlu, İhsan Cihan
Cam, Sefa Burak
Dede, Eda Ciftci
Gizer, Merve
ERGANİŞ, Osman
Sarac, Fahriye
Uzar, Serdar
Enul, Hakan
Adiay, Cumhur
Aykut, Gamze
Polat, Hivda
Yildirim, Ismail Selim
Tekin, Saban
Korukluoglu, Gulay
Zeytin, Hasan Ersin
KORKUSUZ, PETEK
GÜRSEL, İHSAN
Gürsel, Mayda

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Background Vaccines that incorporate multiple SARS-CoV-2 antigens can further broaden the breadth of virus-specific cellular and humoral immunity. This study describes the development and immunogenicity of SARS-CoV-2 VLP vaccine that incorporates the four structural proteins of SARS-CoV-2. Methods VLPs were generated in transiently transfected HEK293 cells, purified by multimodal chromatography, and characterized by tunable-resistive pulse sensing, AFM, SEM, and TEM. Immunoblotting studies verified the protein identities of VLPs. Cellular and humoral immune responses of immunized animals demonstrated the immune potency of the formulated VLP vaccine. Results Transiently transfected HEK293 cells reproducibly generated vesicular VLPs that were similar in size to and expressing all four structural proteins of SARS-CoV-2. Alum adsorbed, K3-CpG ODN-adjuvanted VLPs elicited high titer anti-S, anti-RBD, anti-N IgG, triggered multifunctional Th1-biased T-cell responses, reduced virus load, and prevented lung pathology upon live virus challenge in vaccinated animals. Conclusion These data suggest that VLPs expressing all four structural protein antigens of SARS-CoV-2 are immunogenic and can protect animals from developing COVID-19 infection following vaccination.

URI

https://hdl.handle.net/11511/93749

Journal

ALLERGY

DOI

https://doi.org/10.1111/all.15091

Collections

Department of Biology, Article

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Citation Formats

İ. C. YILMAZ et al., “Development and preclinical evaluation of virus-like particle vaccine against COVID-19 infection,” ALLERGY, pp. 0–0, 2021, Accessed: 00, 2021. [Online]. Available: https://hdl.handle.net/11511/93749.