Development and preclinical evaluation of virus-like particle vaccine against COVID-19 infection

Date

2021-09-01

Author

YILMAZ, İSMAİL CEM
İpekoğlu, Emre Mert
Bulbul, Artun
Turay, Nilsu
Yildirim, Muzaffer
Evcili, Irem
Yilmaz, Naz Surucu
Guvencli, Nese
Aydin, Yagmur
Gungor, Bilgi
Saraydar, Berfu
Bartan, Asli Gulce
Ibibik, Bilgehan
Bildik, Tugce
Baydemir, Ilayda
Sanli, Hatice Asena
Kayaoğlu, Başak
Ceylan, Yasemin
Yildirim, Tugce
Abras, Irem
Ayanoğlu, İhsan Cihan
Cam, Sefa Burak
Dede, Eda Ciftci
Gizer, Merve
ERGANİŞ, Osman
Sarac, Fahriye
Uzar, Serdar
Enul, Hakan
Adiay, Cumhur
Aykut, Gamze
Polat, Hivda
Yildirim, Ismail Selim
Tekin, Saban
Korukluoglu, Gulay
Zeytin, Hasan Ersin
KORKUSUZ, PETEK
GÜRSEL, İHSAN
Gürsel, Mayda

Metadata

Show full item record

This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

Item Usage Stats

196
views

0
downloads

Background Vaccines that incorporate multiple SARS-CoV-2 antigens can further broaden the breadth of virus-specific cellular and humoral immunity. This study describes the development and immunogenicity of SARS-CoV-2 VLP vaccine that incorporates the four structural proteins of SARS-CoV-2. Methods VLPs were generated in transiently transfected HEK293 cells, purified by multimodal chromatography, and characterized by tunable-resistive pulse sensing, AFM, SEM, and TEM. Immunoblotting studies verified the protein identities of VLPs. Cellular and humoral immune responses of immunized animals demonstrated the immune potency of the formulated VLP vaccine. Results Transiently transfected HEK293 cells reproducibly generated vesicular VLPs that were similar in size to and expressing all four structural proteins of SARS-CoV-2. Alum adsorbed, K3-CpG ODN-adjuvanted VLPs elicited high titer anti-S, anti-RBD, anti-N IgG, triggered multifunctional Th1-biased T-cell responses, reduced virus load, and prevented lung pathology upon live virus challenge in vaccinated animals. Conclusion These data suggest that VLPs expressing all four structural protein antigens of SARS-CoV-2 are immunogenic and can protect animals from developing COVID-19 infection following vaccination.

URI

https://hdl.handle.net/11511/93749

Journal

ALLERGY

DOI

https://doi.org/10.1111/all.15091

Collections

Department of Biology, Article

Suggestions

OpenMETU
Core

Differential immune activation following encapsulation of immunostimulatory CpG oligodeoxynucleotide in nanoliposomes. Erikçi, E; Gürsel, Mayda; Gürsel, I (2011-02-01) The immunogenicity of a vaccine formulation is closely related to the effective internalization by the innate immune cells that provide prolonged and simultaneous delivery of antigen and adjuvant to relevant antigen presenting cells. Endosome associated TLR9 recognizes microbial unmethylated CpG DNA. Clinical applications of TLR9 ligands are significantly hampered due to their pre-mature in vivo digestion and rapid clearance. Liposome encapsulation is a powerful tool to increase in vivo stability as well as...
Investigation of the Ligand Binding Characteristics of Staphylococcus aureus NorA Efflux Pump Işık , Esra Büşra; Serçinoğlu, Onur (Orta Doğu Teknik Üniversitesi Enformatik Enstitüsü; 2022-10) Staphylococcus aureus is a gram-positive bacterial pathogen which is highly adaptive to environmental conditions and causes various disorders. Excessive usage of antibiotics may result in development of antibiotic resistance in S. aureus. One of the resistance mechanisms is increase in the activity of transmembrane multi-drug efflux pumps. NorA is the most studied efflux pump in S. aureus, which belongs to Major Facilitator Superfamily (MFS). NorA has been shown to contribute to resistance against a variety...
Development of sandwich type nucleic acid array platform for the detection of micrornas in breast cancer Atılgan, Seren; Öktem, Hüseyin Avni; Department of Biology (2014) MicroRNAs are small non-coding RNAs that are involved in important regulatory pathways such as differentiation, development, metabolism, cell proliferation, and cell death. Several recent research show that deregulated expression of miRNAs has crucial roles in disease pathologies, mainly in cancer. Therefore, it is likely that the usage of miRNAs as diagnostic and prognostic biomarkers in patients and the development of various techniques for the detection of microRNA in clinical research will become widesp...
Investigating the potential of bacillus calmette-guerin vaccine russia strain, cpg oligonucleotides and intravenous immunoglobulin to induce trained immunity in the context of antiviral immunity Baydemir, İlayda; Gürsel, Mayda; Department of Molecular Biology and Genetics (2020-10-12) Innate immune cells undergo metabolic and epigenetic reprogramming in response to specific stimuli, that enable a more robust immune response to secondary exposure to a wide variety of pathogens. This process of innate immune memory development has been termed as Trained Immunity (TI). BCG vaccine is one well-known inducer of innate immune memory. In vivo administration of CpG ODNs or Intravenous Immunoglobulin (IVIg) can also exert heterologous anti-microbial protective immunity. In this thesis, we sought ...
Development of a genetic material transfer approach for gene therapy Ayaz, Şerife; Hasırcı, Vasıf Nejat; Department of Biotechnology (2005) This thesis is focused on the development of a gene delivery system, especially for the purpose of DNA vaccination. DNA expression vectors have the potential to be useful therapeutics for a wide variety of applications. A carrier system was designed to realize the delivery of genes to cells and the promotion of controlled adequate expression in the target cells. The low gene delivery efficiency observed with systems composed of polyplexes is mainly due to low stability of polycation e.g polyethylenimine-DNA...

Citation Formats

İ. C. YILMAZ et al., “Development and preclinical evaluation of virus-like particle vaccine against COVID-19 infection,” ALLERGY, pp. 0–0, 2021, Accessed: 00, 2021. [Online]. Available: https://hdl.handle.net/11511/93749.