Enhancing esophageal repair with bioactive bilayer mesh containing FGF

Date

2021-09-01

Author

Cesur, Ozkan
Endoğan Tanır, Tuğba
Celepli, Pinar
Ozarslan, Fatma
Hucumenoglu, Sema
KARAİBRAHİMOĞLU, Adnan
Hasırcı, Nesrin

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We aimed to prepare a bioactive and biodegradable bilayer mesh formed by fibroblast growth factor (FGF) loaded gelatin film layer, and poly epsilon-caprolactone (PCL) film layer, and to investigate its treatment efficacy on esophageal anastomosis. It is envisaged that the bioactive mesh in in vivo model would improve tissue healing in rats. The full thickness semicircular defects of 0.5 x 0.5 cm(2) were created in anterior walls of abdominal esophagus. The control group had abdominal esophagus isolated with distal esophageal blunt dissection, and sham group had primary anastomosis. In the test groups, the defects were covered with bilayer polymeric meshes containing FGF (5 mu g/2 cm(2)), or not. All rats were sacrificed for histopathology investigation after 7 or 28 days of operation. The groups are coded as FGF(-)-7th day, FGF(+)-7th day, and FGF(+)-28th day, based on their content and operation day. Highest burst pressures were obtained for FGF(+)-7th day, and FGF(+)-28th day groups (p < 0.005) and decreased inflammation grades were observed. Submucosal and muscular collagen deposition scores were markedly increased in these groups compared to sham and FGF(-)-7th day groups having no FGF (p = 0.002, p = 0.001, respectively). It was proved that FGF loaded bioactive bilayer mesh provided effective repair, reinforcement and tissue healing of esophageal defects.

URI

https://hdl.handle.net/11511/93993

Journal

SCIENTIFIC REPORTS

DOI

https://doi.org/10.1038/s41598-021-98840-w

Collections

Test and Measurement Center In advanced Technologies (MERKEZ LABORATUVARI), Article

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Citation Formats

O. Cesur et al., “Enhancing esophageal repair with bioactive bilayer mesh containing FGF,” SCIENTIFIC REPORTS, vol. 11, no. 1, pp. 0–0, 2021, Accessed: 00, 2021. [Online]. Available: https://hdl.handle.net/11511/93993.