The role of bcsE gene in the pathogenicity of Salmonella

Date

2021-08-01

Author

Ozdemir, Caner
AKÇELİK, NEFİSE
Özdemir, Fatma Neslihan
Evcili, Irem
Kahraman, Tamer
GÜRSEL, İHSAN
AKÇELİK, MUSTAFA

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The effects of the bcsE gene and BcsE protein on bacterial physiology and pathogenicity in SalmonellaTyphimurium and Salmonella Group C1 were investigated. It was observed that biofilm and pellicle formation did not occur in the bcsE gene mutants of wild-type strains. Besides, the 'rdar' (red, dry, rough) biofilm morphotype in wild-type strains changed significantly in the mutants. In terms of the bcsE gene, the swimming and swarming motility in mutant strains showed a dramatic increase compared to the wild-type strains. The Salmonella bcsE gene was cloned into Escherichia coli BL21, and the his-tagged protein produced in this strain was purified to obtain polyclonal antibodies in BALB/c mice. The antibodies were showed labeled antigen specificity to the BscE protein. As a result of immunization and systemic persistence tests carried out with BALB/c mice, BscE protein was determined to trigger high levels of humoral and cellular responses (Th1 cytokine production, IgG2a/IgG1 > 1). Systemic persistence in the liver and spleen samples decreased by 99.99% and 100% in the bcsE mutant strains. Finally, invasion abilities on HT-29 epithelial cells of wild-type strains were utterly disappeared in their bcsE gene mutant strains.This article describes the role of BcsE, which is a c-di-GMP binding protein, in Salmonella biofilm formation properties and pathogenicity.

Subject Keywords

Salmonella, BcsE, Biofilm, Invasion, Persistence, Pathogenicity

URI

https://hdl.handle.net/11511/94357

Journal

PATHOGENS AND DISEASE

DOI

https://doi.org/10.1093/femspd/ftab037

Collections

Department of Food Engineering, Article

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Citation Formats

C. Ozdemir et al., “The role of bcsE gene in the pathogenicity of Salmonella,” PATHOGENS AND DISEASE, vol. 79, no. 6, pp. 0–0, 2021, Accessed: 00, 2021. [Online]. Available: https://hdl.handle.net/11511/94357.