Identification of an mRNA isoform switch for HNRNPA1 in breast cancers.

Download

index.pdf

Date

2021-12-27

Author

Erdem, Murat
Ozgul, İbrahim
Dioken, Didem Naz
Gurcuoglu, Irmak
Guntekin Ergun, Sezen
Cetin-Atalay, Rengul
Can, Tolga
Erson Bensan, Ayşe Elif

Metadata

Show full item record

This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

Item Usage Stats

250
views

92
downloads

Roles of HNRNPA1 are beginning to emerge in cancers; however, mechanisms causing deregulation of HNRNPA1 function remain elusive. Here, we describe an isoform switch between the 3′-UTR isoforms of HNRNPA1 in breast cancers. We show that the dominantly expressed isoform in mammary tissue has a short half-life. In breast cancers, this isoform is downregulated in favor of a stable isoform. The stable isoform is expressed more in breast cancers, and more HNRNPA1 protein is synthesized from this isoform. High HNRNPA1 protein levels correlate with poor survival in patients. In support of this, silencing of HNRNPA1 causes a reversal in neoplastic phenotypes, including proliferation, clonogenic potential, migration, and invasion. In addition, silencing of HNRNPA1 results in the downregulation of microRNAs that map to intragenic regions. Among these miRNAs, miR-21 is known for its transcriptional upregulation in breast and numerous other cancers. Altogether, the cancer-specifc isoform switch we describe here for HNRNPA1 emphasizes the need to study gene expression at the isoform level in cancers to identify novel cases of oncogene activation.

URI

https://hdl.handle.net/11511/95069

Journal

Scientific reports

DOI

https://doi.org/10.1038/s41598-021-04007-y

Collections

Department of Biology, Article

Suggestions

OpenMETU
Core

HNRNPA1 Isoform Switch in Breast Cancers Erson Bensan, Ayşe Elif; Özgül, İbrahim; Erdem, Murat; Döken, Didem Naz (2021-11-01) Roles of HNRNPA1 are beginning to emerge in cancers; however, mechanisms causing deregulation of HNRNPA1 function remain elusive. Here, we identify and characterize an isoform switch between the 3’-UTR isoforms of HNRNPA1. We show that the dominantly expressed isoform in mammary tissue has a short half-life. In breast cancers, this isoform is downregulated, whereas a stable isoform is expressed more. As a result, the HNRNPA1 protein is upregulated. High HNRNPA1 protein levels correlate with poor survival in...
Identification of Potential Therapeutic Genes and Pathways in Phytoestrogen Emodin Treated Breast Cancer Cell Lines via Network Biology Approaches Sakalli-Tecim, Elif; Uyar-Arpaci, Pembegul; Güray, Nülüfer Tülün (2021-02-01) Phytoestrogens have been investigated for their potential anti-tumorigenic effects in various cancers including breast cancer. Emodin being a phytoestrogen shows anti-carcinogenic properties especially in estrogen receptor positive (ER+) breast cancers. The aim of this study is to identify the molecular mechanism and related biological pathways in both (ER+) MCF-7 and (ER-) MDA-MB-231 breast cancer cell lines upon Emodin treatment via microarray analysis in order to find out therapeutic biomarkers. In both ...
Evaluation of metabolite variation between acute lymphoblastic leukemia (ALL) and chronic myeloidleukemia (CML) cell lines via triple quadrupole LC-MS Pekşen, Ceren; Özen, Can.; Department of Biotechnology (2019) Hematologic cancers have two origins: myeloid and lymphoid. While lymphatic leukemia, originate from the lymphoid cell line, acute myeloid leukemia (AML) and chronic myeloid leukemia (CML) originate from the myeloid cell line. CML is granulocyte cancer and in this disease, as well as granulocytes, the number of cells that cause blood clotting, which are called platelets, may increase in the blood. In CML, a non-hereditary genetic abnormality, Philadelphia chromosome, where a structural change occurs in chro...
Identification of gene mutations involved in drug resistance in liver cancer using RNA-SEQ data analysis Shojaei, Mona; Atalay, Rengül; Acar, Aybar Can; Department of Bioinformatics (2016) A significant concern in cancer research is the detection of cancer associated somatic mutations. Liver cancer is the 5th most common and 2nd deadliest cancer in the world. Several somatic mutations were previously reported in liver cancer but their relations to chemotherapeutic response was not studied in detail. In this study, the relationship between mutation status and drug treatment response of well-differentiated Huh7 and poorly-differentiated Mahlavu liver cancer cells were analyzed. The RNA-Seq data...
Identification of rare cell types in lung cancer and investigation of their use in prognosis Yıldız, Cemal; Şengül Babal, Simge; Pir, Pınar (Orta Doğu Teknik Üniversitesi Enformatik Enstitüsü; 2022-10) Cancer is a complex, dynamic and heterogeneous disease. Identification of tumor heterogeneity holds a significant promise not only on tumor classification but also on predicting the prognosis and choosing the right treatment. Therefore, it is essential to investigate tumor heterogeneity in a regular diagnosis procedure. Tumors contain variety of cell types such as malignant cells, non-malignant cells, immune cells, and rare cells (i.e., dormant cells (DRM) and cancer stem cells (CSC))....

Citation Formats

M. Erdem et al., “Identification of an mRNA isoform switch for HNRNPA1 in breast cancers.,” Scientific reports, vol. 11, no. 1, pp. 24444–24444, 2021, Accessed: 00, 2021. [Online]. Available: https://hdl.handle.net/11511/95069.