Show/Hide Menu
Hide/Show Apps
Logout
Türkçe
Türkçe
Search
Search
Login
Login
OpenMETU
OpenMETU
About
About
Open Science Policy
Open Science Policy
Open Access Guideline
Open Access Guideline
Postgraduate Thesis Guideline
Postgraduate Thesis Guideline
Communities & Collections
Communities & Collections
Help
Help
Frequently Asked Questions
Frequently Asked Questions
Guides
Guides
Thesis submission
Thesis submission
MS without thesis term project submission
MS without thesis term project submission
Publication submission with DOI
Publication submission with DOI
Publication submission
Publication submission
Supporting Information
Supporting Information
General Information
General Information
Copyright, Embargo and License
Copyright, Embargo and License
Contact us
Contact us
Microbial growth and attachment of Salmonella and enterohemorrhagic and enteroaggregative Escherichia coli strains on cress microgreens grown in peat soil system
Date
2021-12-01
Author
Namlı, Şahin
Samut, Hilal
Soyer, Yeşim
Metadata
Show full item record
This work is licensed under a
Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License
.
Item Usage Stats
218
views
0
downloads
Cite This
Purpose This study aimed to investigate how enteric pathogens and their biofilm populations on fresh produce survive according to time that contamination has occurred on leaves and contamination route: seed irrigation water. Design/methodology/approach Cress was contaminated in two different ways: contamination of seeds and irrigation water with 8-log MPN/mL bacterial load, Salmonella Newport, Escherichia coli O157:H7, O104:H4 or O78:H2. While contaminated seeds were cultivated for seed contamination, contaminated irrigation was applied at the end of each week to separate groups of samples obtained from cultivated surface-sterile seeds to understand how long these pathogens could survive until harvest. Findings The results indicated these pathogens survived until harvest, and formed biofilms on cress leaves grown using both contaminated seeds and irrigation water. No significant difference was observed among populations of Salmonella and E. coli groups in terms of survival (similar to 4.5-6.0 log MPN/g) and biofilm formation (similar to 4.4-5.7 log MPN/g) for contamination by seed. Also, SEM images revealed biofilm-like structures, the proofs of the attachment of these pathogens on leaf surfaces. Originality/value From our knowledge this is the first study focusing on the survival and biofilm formation of one Salmonella serotype (Newport) and three E. coli serotypes (O157:H7, O104:H4, and O78:H2), representing enterohemorrhagic and enteroaggregative E. coli pathogenic subgroups, under the same irrigation and growth schemes. Furthermore, this study mimics the contamination of seeds and irrigation water with sewage or wastewater and may shed light on contamination of fresh produce grown using poor wastewater treatment.
Subject Keywords
Salmonella
,
E
,
coli
,
Biofilm
,
Attachment
,
Seed contamination
,
Fresh produce
,
Cress
,
Microgreen
,
Irrigation
,
CONTAMINATED MANURE COMPOSTS
,
HEMOLYTIC-UREMIC SYNDROME
,
HUMAN ENTERIC PATHOGENS
,
LEAFY GREEN VEGETABLES
,
CLIMATE-CHANGE
,
O157H7
,
SURVIVAL
,
LEAVES
,
PERSISTENCE
,
PREHARVEST
URI
https://hdl.handle.net/11511/95131
Journal
BRITISH FOOD JOURNAL
DOI
https://doi.org/10.1108/bfj-03-2021-0269
Collections
Department of Food Engineering, Article
Suggestions
OpenMETU
Core
PHAGE THERAPY AGAINST CONTAMINATION OF PATHOGENIC ESCHERICHIA COLI STRAINS (O104:H4, O157:H7, AND O26) DURING GARDEN CRESS (LEPIDIUM SATIVUM LINN.) VEGETATION
İskender, İrem; Soyer, Yeşim; Önde, Sertaç; Department of Biotechnology (2022-8-24)
The number of outbreaks caused by foodborne pathogens due to contaminated fresh produces has increased worldwide. Escherichia coli is one of the most causative foodborne pathogens. The major transmission way of foodborne pathogens to fresh produce is irrigation water. Once foodborne pathogens are transmitted, the elimination of pathogens from the fresh produce is harder. The most commonly used disinfected agents are chlorine-derived compounds for fresh produce. Yet, usage of them has been related to increas...
Biosecurity of agricultural product through speciation of pesticides: Ayaş tomato
Sifatullah, KM; Tuncel, Semra; Tuncel, Süleyman Gürdal; Department of Earth System Science (2014)
Speciation of chlorinated pesticides in soil-water-tomato plant is researched in connection with food security. For this purpose 16 soil, 16 tomato and 4 irrigation water samples were collected from selected two fields in Ayaş, Ankara, Turkey and analyzed with GC-MS system. Soil and tomato samples were extracted by using ultrasonic bath. On the other hand, water samples were extracted by using solid phase extraction. The extraction recoveries were determined as %64.88, %42.03, and %53.36 for tomato, water, ...
Biomanipulation as a Restoration Tool to Combat Eutrophication: Recent Advances and Future Challenges
Jeppesen, Erik; Sondergaard, Martin; Lauridsen, Torben L.; Davidson, Thomas A.; Liu, Zhengwen; Mazzeo, Nestor; Trochine, Carolina; Özkan, Korhan; Jensen, Henning S.; Trolle, Dennis; Starling, Fernando; Lazzaro, Xavier; Johansson, Liselotte S.; Bjerring, Rikke; Liboriussen, Lone; Larsen, Soren E.; Landkildehus, Frank; Egemose, Sara; Meerhoff, Mariana (2012-01-01)
Eutrophication resulting from high nutrient loading has been the paramount environmental problem for lakes world-wide for the past four decades. Efforts are being made in many parts of the world to reduce external nutrient loading via improved wastewater treatment or diversion of nutrient-rich inflows. However, even after a reduction of the external phosphorus loading, the effects obtained may be unsatisfactory. This may reflect an insufficient reduction in the external nutrient loading to effectively limit...
Coupled wastewater treatment and CO₂ mitigation by microalgal (Chlorella Vulgaris) cultures
Çaylı, Direniş; Demirer, Göksel Niyazi; Bayramoğlu, Tuba Hande; Department of Earth System Science (2017)
Eutrophication, ecosystem damage and poor water quality predominantly are among the major problems which are brought about by excess nitrogen and phosphorus discharged to receiving environments by different wastewaters. Nutrient (mainly nitrogen and phosphorus) removal from wastewaters is still an unsolved problem in many countries, including Turkey. For example, the ratio of wastewaters which are subjected to tertiary (advanced) wastewater treatment is around 38.3% (Turkish Statistical Institute, 2012). Th...
Anaerobic treatability and biogas production potential of pistachio processing wastewater with upflow anaerobic sludge blanket (uasb) reactor
Gür, Eray; Demirer, Göksel Niyazi; Department of Environmental Engineering (2016)
Agro-industrial wastes cannot be treated in conventional wastewater treatment plants due to high organic contents. However, they have a high potential as renewable energy resources, and can be converted into valuable end products via application of appropriate treatment technologies. Anaerobic digestion (AD) is a process which converts organic materials into methane and carbon dioxide in the absence of oxygen. Therefore, wellestablished anaerobic biotechnologies, such as up-flow anaerobic sludge blanket (UA...
Citation Formats
IEEE
ACM
APA
CHICAGO
MLA
BibTeX
Ş. Namlı, H. Samut, and Y. Soyer, “Microbial growth and attachment of Salmonella and enterohemorrhagic and enteroaggregative Escherichia coli strains on cress microgreens grown in peat soil system,”
BRITISH FOOD JOURNAL
, pp. 0–0, 2021, Accessed: 00, 2022. [Online]. Available: https://hdl.handle.net/11511/95131.