Microbial growth and attachment of Salmonella and enterohemorrhagic and enteroaggregative Escherichia coli strains on cress microgreens grown in peat soil system

Purpose This study aimed to investigate how enteric pathogens and their biofilm populations on fresh produce survive according to time that contamination has occurred on leaves and contamination route: seed irrigation water. Design/methodology/approach Cress was contaminated in two different ways: contamination of seeds and irrigation water with 8-log MPN/mL bacterial load, Salmonella Newport, Escherichia coli O157:H7, O104:H4 or O78:H2. While contaminated seeds were cultivated for seed contamination, contaminated irrigation was applied at the end of each week to separate groups of samples obtained from cultivated surface-sterile seeds to understand how long these pathogens could survive until harvest. Findings The results indicated these pathogens survived until harvest, and formed biofilms on cress leaves grown using both contaminated seeds and irrigation water. No significant difference was observed among populations of Salmonella and E. coli groups in terms of survival (similar to 4.5-6.0 log MPN/g) and biofilm formation (similar to 4.4-5.7 log MPN/g) for contamination by seed. Also, SEM images revealed biofilm-like structures, the proofs of the attachment of these pathogens on leaf surfaces. Originality/value From our knowledge this is the first study focusing on the survival and biofilm formation of one Salmonella serotype (Newport) and three E. coli serotypes (O157:H7, O104:H4, and O78:H2), representing enterohemorrhagic and enteroaggregative E. coli pathogenic subgroups, under the same irrigation and growth schemes. Furthermore, this study mimics the contamination of seeds and irrigation water with sewage or wastewater and may shed light on contamination of fresh produce grown using poor wastewater treatment.


İskender, İrem; Soyer, Yeşim; Önde, Sertaç; Department of Biotechnology (2022-8-24)
The number of outbreaks caused by foodborne pathogens due to contaminated fresh produces has increased worldwide. Escherichia coli is one of the most causative foodborne pathogens. The major transmission way of foodborne pathogens to fresh produce is irrigation water. Once foodborne pathogens are transmitted, the elimination of pathogens from the fresh produce is harder. The most commonly used disinfected agents are chlorine-derived compounds for fresh produce. Yet, usage of them has been related to increas...
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Speciation of chlorinated pesticides in soil-water-tomato plant is researched in connection with food security. For this purpose 16 soil, 16 tomato and 4 irrigation water samples were collected from selected two fields in Ayaş, Ankara, Turkey and analyzed with GC-MS system. Soil and tomato samples were extracted by using ultrasonic bath. On the other hand, water samples were extracted by using solid phase extraction. The extraction recoveries were determined as %64.88, %42.03, and %53.36 for tomato, water, ...
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Agro-industrial wastes cannot be treated in conventional wastewater treatment plants due to high organic contents. However, they have a high potential as renewable energy resources, and can be converted into valuable end products via application of appropriate treatment technologies. Anaerobic digestion (AD) is a process which converts organic materials into methane and carbon dioxide in the absence of oxygen. Therefore, wellestablished anaerobic biotechnologies, such as up-flow anaerobic sludge blanket (UA...
Citation Formats
Ş. Namlı, H. Samut, and Y. Soyer, “Microbial growth and attachment of Salmonella and enterohemorrhagic and enteroaggregative Escherichia coli strains on cress microgreens grown in peat soil system,” BRITISH FOOD JOURNAL, pp. 0–0, 2021, Accessed: 00, 2022. [Online]. Available: https://hdl.handle.net/11511/95131.