Hybrid-architectured promoter design to deregulate expression in yeast

Date

2021-01-01

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Gündüz Ergün, Burcu
Çalık, Pınar

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This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

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https://hdl.handle.net/11511/95308

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Methods in Enzymology

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Department of Chemical Engineering, Book / Book chapter

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Hybrid-architectured promoter design to deregulate expression in yeast Ergun, Burcu Gunduz; Çalık, Pınar (2021-01-01) Hybrid-architectured promoter design to deregulate expression in yeast under modulating power of carbon sources involves replacing native cis-acting DNA sequence(s) with de novo synthetic tools in coordination with master regulator transcription factor (TF) to alter crosstalk between signaling pathways, and consequently, transcriptionally rewire the expression. Hybrid-promoter architectures can be designed to mimic native promoter architectures in yeast's preferred carbon source utilization pathway. The met...
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Hybrid-architectured promoter design to engineer expression in yeast Ergun, Burcu Gunduz; Çalık, Pınar (2021-01-01) Engineered promoters are key components that allow engineered expression of genes in the cell-factory design. Promoters having exceptional strength are attractive candidates for designing metabolic engineering strategies for tailoring de novo production strategies that require directed evolution methods by engineering with de novo synthetic biology tools. Engineered promoter variants (EPVs) of naturally occurring promoters (NOPs) can be designed using metabolic engineering strategies and synthetic biology t...
Hybrid-architectured double-promoter expression systems enhance and upregulate-deregulated gene expressions in Pichia pastoris in methanol-free media Demir, Irem; Çalık, Pınar (Springer Science and Business Media LLC, 2020-10-01) Double-promoter expression system (DPES) design as de novo metabolic engineering strategy enables fine-tuned and enhanced gene expression. We constructed a collection of monodirectional hybrid-architectured DPESs with engineered promoter variants P(ADH2-Cat8-L2)and P(mAOX1)and with the naturally occurring promoter P(GAP)to enhance and upregulate-deregulated gene expressions inPichia pastorisin methanol-free media. Reporter red fluorescent protein (mApple) and enhanced green fluorescent protein (eGFP) were e...
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Citation Formats

B. Gündüz Ergün and P. Çalık, Hybrid-architectured promoter design to deregulate expression in yeast. 2021.