Hybrid-architectured promoter design to deregulate expression in yeast

Date

2021-01-01

Author

Gündüz Ergün, Burcu
Çalık, Pınar

Metadata

Show full item record

This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

Item Usage Stats

122
views

0
downloads

URI

https://hdl.handle.net/11511/95308

Relation

Methods in Enzymology

Collections

Department of Chemical Engineering, Book / Book chapter

Suggestions

OpenMETU
Core

Hybrid-architectured promoter design to deregulate expression in yeast Ergun, Burcu Gunduz; Çalık, Pınar (2021-01-01) Hybrid-architectured promoter design to deregulate expression in yeast under modulating power of carbon sources involves replacing native cis-acting DNA sequence(s) with de novo synthetic tools in coordination with master regulator transcription factor (TF) to alter crosstalk between signaling pathways, and consequently, transcriptionally rewire the expression. Hybrid-promoter architectures can be designed to mimic native promoter architectures in yeast's preferred carbon source utilization pathway. The met...
Hybrid-architectured promoter design to engineer expression in yeast Gündüz Ergün, Burcu; Çalık, Pınar (Academic Press Inc., 2021-01-01) Engineered promoters are key components that allow engineered expression of genes in the cell-factory design. Promoters having exceptional strength are attractive candidates for designing metabolic engineering strategies for tailoring de novo production strategies that require directed evolution methods by engineering with de novo synthetic biology tools. Engineered promoter variants (EPVs) of naturally occurring promoters (NOPs) can be designed using metabolic engineering strategies and synthetic biology t...
Hybrid-architectured promoter design to engineer expression in yeast Ergun, Burcu Gunduz; Çalık, Pınar (2021-01-01) Engineered promoters are key components that allow engineered expression of genes in the cell-factory design. Promoters having exceptional strength are attractive candidates for designing metabolic engineering strategies for tailoring de novo production strategies that require directed evolution methods by engineering with de novo synthetic biology tools. Engineered promoter variants (EPVs) of naturally occurring promoters (NOPs) can be designed using metabolic engineering strategies and synthetic biology t...
Hybrid-architectured double-promoter expression systems enhance and upregulate-deregulated gene expressions in Pichia pastoris in methanol-free media Demir, Irem; Çalık, Pınar (Springer Science and Business Media LLC, 2020-10-01) Double-promoter expression system (DPES) design as de novo metabolic engineering strategy enables fine-tuned and enhanced gene expression. We constructed a collection of monodirectional hybrid-architectured DPESs with engineered promoter variants P(ADH2-Cat8-L2)and P(mAOX1)and with the naturally occurring promoter P(GAP)to enhance and upregulate-deregulated gene expressions inPichia pastorisin methanol-free media. Reporter red fluorescent protein (mApple) and enhanced green fluorescent protein (eGFP) were e...
Hybrid Genetic Algorithm with Simulated Annealing for Resource-Constrained Project Scheduling BETTEMİR, ÖNDER HALİS; Sönmez, Rifat (American Society of Civil Engineers (ASCE), 2015-09-01) Resource-constrained project scheduling problem (RCPSP) is a very important optimization problem in construction project management. Despite the importance of the RCPSP in project scheduling and management, commercial project management software provides very limited capabilities for the RCPSP. In this paper, a hybrid strategy based on genetic algorithms, and simulated annealing is presented for the RCPSP. The strategy aims to integrate parallel search ability of genetic algorithms with fine tuning capabili...

Citation Formats

B. Gündüz Ergün and P. Çalık, Hybrid-architectured promoter design to deregulate expression in yeast. 2021.