Antibacterial properties and osteoblast interactions of microfluidically synhtesized chitosan – spion composite nanoparticles under external static magnetic field

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FBE-Melisa Kafalı Tez 160922.pdf

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2022-8-18

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Kafalı, Melisa

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In this research, a multistep microfluidic reactor was used to fabricate chitosan superparamagnetic iron oxide composite nanoparticles (Ch - SPIONs) to develop antibacterial agents that can be targeted to infection foci and visualized with magnetic resonance imaging. Monodispersed Ch – SPIONs had an average particle size of 8.8 ± 1.2 nm with a magnetization value of 32.0 emu/g. These nanoparticles were used to fight against Staphylococcus aureus (S. aureus) and Pseudomonas aeruginosa (P. aeruginosa), which are dangerous pathogens that cause tissue and biomedical device-related infection. While Ch – SPIONs showed up to 2 – fold reduction in colonies for both bacteria strains at 0.1 g/L concentration, up to 5 – fold reduction in colonies was observed upon the application of 0.4 T magnetic field for the same concentration of nanoparticles. In addition, osteoblasts were viable and proliferated up to 7 days upon their interaction with Ch – SPIONs in vitro both in the absence and presence of magnetic field. Lastly, Ch – SPIONs reduced T1 and T2 signal intensity in magnetic resonance imaging (MRI). Results cumulatively showed that Ch – SPIONs is a potential candidate as a cytocompatible and antibacterial agent that can be targeted to biofilm bacteria and imaged using MRI.

Subject Keywords

Microfluidics, Antibacterial, Magnetic Field, Osteoblast, Magnetic Resonance Imaging

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https://hdl.handle.net/11511/99426

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Graduate School of Natural and Applied Sciences, Thesis

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Citation Formats

M. Kafalı, “Antibacterial properties and osteoblast interactions of microfluidically synhtesized chitosan – spion composite nanoparticles under external static magnetic field,” M.S. - Master of Science, Middle East Technical University, 2022.