Antibacterial properties and osteoblast interactions of microfluidically synhtesized chitosan – spion composite nanoparticles under external static magnetic field

Kafalı, Melisa
In this research, a multistep microfluidic reactor was used to fabricate chitosan superparamagnetic iron oxide composite nanoparticles (Ch - SPIONs) to develop antibacterial agents that can be targeted to infection foci and visualized with magnetic resonance imaging. Monodispersed Ch – SPIONs had an average particle size of 8.8 ± 1.2 nm with a magnetization value of 32.0 emu/g. These nanoparticles were used to fight against Staphylococcus aureus (S. aureus) and Pseudomonas aeruginosa (P. aeruginosa), which are dangerous pathogens that cause tissue and biomedical device-related infection. While Ch – SPIONs showed up to 2 – fold reduction in colonies for both bacteria strains at 0.1 g/L concentration, up to 5 – fold reduction in colonies was observed upon the application of 0.4 T magnetic field for the same concentration of nanoparticles. In addition, osteoblasts were viable and proliferated up to 7 days upon their interaction with Ch – SPIONs in vitro both in the absence and presence of magnetic field. Lastly, Ch – SPIONs reduced T1 and T2 signal intensity in magnetic resonance imaging (MRI). Results cumulatively showed that Ch – SPIONs is a potential candidate as a cytocompatible and antibacterial agent that can be targeted to biofilm bacteria and imaged using MRI.


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Çıkrıkcı, Sevil; Öztop, Halil Mecit; Department of Food Engineering (2019)
Nuclear magnetic resonance (NMR) and Magnetic Resonance Imaging (MRI) are well-known non-invasive characterization methods used in a wide range of areas; from medical to food applications. NMR experiments are conducted either through spectroscopy with high resolution systems or with relaxometery (Time Domain NMR) through mid or low field systems. Time domain NMR is primarily based on relaxation times and diffusion measurements from the signal coming from the whole sample while MRI enables to visualize the i...
Magnetohydrodynamic Flow Imaging Using Spin-Echo Pulse Sequence
Eroğlu, Hasan Hüseyin; SADIGHI, MEHDI; Eyüboğlu, Behçet Murat (2019-04-24)
In this study, magnetohydrodynamic (MHD) flow of conductive liquids due to injection of electrical current during magnetic resonance imaging (MRI) is investigated. A spin-echo based MRI pulse sequence is proposed to image the MHD flow. Magnetic resonance (MR) phase effects of the MHD flow is related to the MRI pulse parameters and injected current. Average velocity distributions of the MHD flow are reconstructed using the MR phase images. The method is validated by numerical simulations. The reconstruction ...
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Yılmaz, Ayşen; Eyueboglu, B. M. (2006-09-01)
Conventional Magnetic Resonance Imaging (MRI) techniques require homogeneous main magnetic fields. However, MRI applications that are executed in inhomogenous main magnetic fields have been developed in recent years. In this study, RF coil geometries are designed for MRI applications in inhomogeneous magnetic fields. Method of moments is used to obtain the current density distribution on a predefined surface that can produce a desired magnetic field, which is perpendicular to the given inhomogenous main mag...
Synthesis and characterization of ultra-small gadolinium oxide nanoparticles
Tasman, Pakizan; Volkan, Mürvet; Department of Chemistry (2017)
Ultra-small gadolinium oxide nanoparticles are used as contrast agents for molecular and cellular magnetic resonance imaging (MRI) procedure. They are also important for drug targeting magnetic separation and gene therapy applications due to their paramagnetic properties. Gadolinium oxide nanoparticles have been known to have the highest gadolinium density of all paramagnetic gadolinium contrast agents since they generate strong positive contrast enhancement. There are many techniques that have been introdu...
Magnetic Resonance Signal Analysis in Inhomogenous Magnetic Fields
Arpinar, V. E.; Eyüboğlu, Behçet Murat (2009-09-12)
Nuclear Magnetic Resonance (NMR) systems with inhomogenous main magnetic fields have been satisfactorily used to explore material properties. So that, imaging of biological tissues using Magnetic Resonance Imaging (MRI) systems with inhomogenous main magnetic fields could be explored. In this work, magnetic resonance (MR) signal deviation due to inhomogeneity in the main magnetic field of a MRI system is investigated. This analysis gives the understanding of the effect of inhomogeneity in magnetic field to ...
Citation Formats
M. Kafalı, “Antibacterial properties and osteoblast interactions of microfluidically synhtesized chitosan – spion composite nanoparticles under external static magnetic field,” M.S. - Master of Science, Middle East Technical University, 2022.