Enzymatic synthesis of novel dipeptides.

Tüzün, Işıl


Enzymatic Mineralization of Hydrogels for Bone Tissue Engineering by Incorporation of Alkaline Phosphatase
Douglas, Timothy E. L.; Messersmith, Philip B.; Chasan, Safak; Mikos, Antonios G.; de Mulder, Eric L. W.; Dickson, Glenn; Schaubroeck, David; Balcaen, Lieve; Vanhaecke, Frank; Dubruel, Peter; Jansen, John A.; Leeuwenburgh, Sander C. G. (Wiley, 2012-08-01)
Alkaline phosphatase (ALP), an enzyme involved in mineralization of bone, is incorporated into three hydrogel biomaterials to induce their mineralization with calcium phosphate (CaP). These are collagen type I, a mussel-protein-inspired adhesive consisting of PEG substituted with catechol groups, cPEG, and the PEG/fumaric acid copolymer OPF. After incubation in Ca-GP solution, FTIR, EDS, SEM, XRD, SAED, ICP-OES, and von Kossa staining confirm CaP formation. The amount of mineral formed decreases in the orde...
Enzymic activity of the K5-type yeast killer toxin and its characterization
Izgu, F; Altinbay, D; Sertkaya, A (Informa UK Limited, 2005-11-01)
K5-type yeast killer toxin secreted by P. anomala NCYC 434 cells has a broad killing spectrum. Competitive inhibiton of killer activity showed that glucans, mainly the beta-1,3 glucan, represent the primary toxin binding site within the cell wall of sensitive cells. Its hydrolytic activity on laminarin in an exo-like fashion revealed that the toxin exerts its killing effect by exo-beta-1,3-glucanase activity. Its specific activity on laminarin was 120U/mg, and the Michaelis constants K-m, and V-max for lami...
Enzymatic extraction of activated sludge extracellular polymers and implications on bioflocculation
Sesay, Ml; Özcengiz, Gülay; Sanin, Faika Dilek (Elsevier BV, 2006-04-01)
This study examines enzyme hydrolysis, a mild, effective, but a rarely used method of extracellular polymer extraction, in removing polymers from mixed culture activated sludge flocs. Two carbohydrate specific enzymes (a-amylase and cellulase) and a protein specific enzyme (proteinase) are used during the study. First, the kinetic aspect is investigated, then enzyme dose optimization is carried out on laboratory grown activated sludge samples cultured at solids retention times (SRT) of 4 and 20 days. A more...
Enzymatically induced mineralization of platelet-rich fibrin
Douglas, Timothy E. L.; Gassling, Volker; Declercq, Heidi A.; Purcz, Nicolai; Pamula, Elzbieta; Haugen, Havard J.; Chasan, Safak; de Mulder, Eric L. W.; Jansen, John A.; Leeuwenburgh, Sander C. G. (Wiley, 2012-05-01)
Membranes of the autologous blood-derived biomaterial platelet-rich fibrin (PRF) were functionalized by incorporation of alkaline phosphatase (ALP), an enzyme involved in mineralization of bone, and subsequently incubated in calcium glycerophosphate (CaGP) solution to induce PRFs mineralization with calcium phosphate (CaP) to improve PRFs suitability as a material for bone replacement. Incorporated ALP retained its bioactivity and induced formation of CaP material within PRF membranes, as confirmed by SEM, ...
Enzymatic resolution of (+/-)-2-endo-hydroxymethyl and acetoxymethyl substituted hexachloronorbornene derivatives
TÜRKMEN, YUNUS EMRE; Akhmedov, IM; Tanyeli, Cihangir (Elsevier BV, 2005-07-04)
(+/-)-2-endo-Hydroxymethyl-1,4,5,6,7,7-hexachlorobicyclo[2.2.1]hept-5-ene and (+/-)-2-endo-acetoxymethyl-1,4,5,6,7,7hexachlorobicyclo[2.2.1]hept-5-ene were resolved by using various hydrolases to afford enantiomerically enriched products with ees of 94-98%. The absolute configuration was determined by transforming 2-endo-acetoxymethyl-1,4,5,6,7,7-hexachlorobicyclo[2.2.1]hept-5-ene into 2-endo-hydroxymetliyl-bicyclo[2.2.1]hept-5-ene with known absolute configuration.
Citation Formats
I. Tüzün, “Enzymatic synthesis of novel dipeptides.,” Middle East Technical University, 2002.