Enzymatic Mineralization of Hydrogels for Bone Tissue Engineering by Incorporation of Alkaline Phosphatase

Douglas, Timothy E. L.
Messersmith, Philip B.
Chasan, Safak
Mikos, Antonios G.
de Mulder, Eric L. W.
Dickson, Glenn
Schaubroeck, David
Balcaen, Lieve
Vanhaecke, Frank
Dubruel, Peter
Jansen, John A.
Leeuwenburgh, Sander C. G.
Alkaline phosphatase (ALP), an enzyme involved in mineralization of bone, is incorporated into three hydrogel biomaterials to induce their mineralization with calcium phosphate (CaP). These are collagen type I, a mussel-protein-inspired adhesive consisting of PEG substituted with catechol groups, cPEG, and the PEG/fumaric acid copolymer OPF. After incubation in Ca-GP solution, FTIR, EDS, SEM, XRD, SAED, ICP-OES, and von Kossa staining confirm CaP formation. The amount of mineral formed decreases in the order cPEG?>?collagen?>?OPF. The mineral:polymer ratio decreases in the order collagen?>?cPEG?>?OPF. Mineralization increases Young's modulus, most profoundly for cPEG. Such enzymatically mineralized hydrogel/CaP composites may find application as bone regeneration materials.


Enzymatically induced mineralization of platelet-rich fibrin
Douglas, Timothy E. L.; Gassling, Volker; Declercq, Heidi A.; Purcz, Nicolai; Pamula, Elzbieta; Haugen, Havard J.; Chasan, Safak; de Mulder, Eric L. W.; Jansen, John A.; Leeuwenburgh, Sander C. G. (Wiley, 2012-05-01)
Membranes of the autologous blood-derived biomaterial platelet-rich fibrin (PRF) were functionalized by incorporation of alkaline phosphatase (ALP), an enzyme involved in mineralization of bone, and subsequently incubated in calcium glycerophosphate (CaGP) solution to induce PRFs mineralization with calcium phosphate (CaP) to improve PRFs suitability as a material for bone replacement. Incorporated ALP retained its bioactivity and induced formation of CaP material within PRF membranes, as confirmed by SEM, ...
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Izgu, F; Altinbay, D; Sertkaya, A (Informa UK Limited, 2005-11-01)
K5-type yeast killer toxin secreted by P. anomala NCYC 434 cells has a broad killing spectrum. Competitive inhibiton of killer activity showed that glucans, mainly the beta-1,3 glucan, represent the primary toxin binding site within the cell wall of sensitive cells. Its hydrolytic activity on laminarin in an exo-like fashion revealed that the toxin exerts its killing effect by exo-beta-1,3-glucanase activity. Its specific activity on laminarin was 120U/mg, and the Michaelis constants K-m, and V-max for lami...
HACIOGLU, B; Akbulut, Ural; Toppare, Levent Kamil (Wiley, 1989-10-01)
Electroinitiated polymerization of acrylamide was carried out in acetonitrile–tetrabutylammonium fluoroborate by electrolytic reduction of monomer. It was shown by cyclic voltammetry that direct electron transfer from the cathode to the monomer can be achieved in this solvent–electrolyte system. Reduction peak potentials measured by cyclic voltammetry indicated that sodium salts will interfere with such a mechanism. Since the reduction peak potential of sodium salt and dimethylformamide are found to be lowe...
Electroactive macromonomers based on pyrrole and thiophene: a versatile route to conducting block and graft polymers
Yagci, Y; Toppare, Levent Kamil (Wiley, 2003-10-01)
The synthesis of block and graft copolymers containing insulating and conducting polypyrrole or polythiophene segments via a two-step procedure is described. Synthetic pathways to prepare pyrrole and thiophene functional macromonomers involving conventional and controlled/living polymerizations such as anionic and cationic ring opening, atom transfer radical polymerization are discussed. The use of these macromonomers in subsequent electrochemical and chemical polymerizations, leading to the formation of co...
Trehalose Glycopolymers as Excipients for Protein Stabilization
Lee, Juneyoung; Lin, En-Wei; Lau, Uland Y.; Hedrick, James L.; Bat, Erhan; Maynard, Heather D. (American Chemical Society (ACS), 2013-08-01)
Herein, the synthesis of four different trehalose glycopolymers and investigation of their ability to stabilize proteins to heat and lyophilization stress are described. The disaccharide, alpha,alpha-trehalose, was modified with a styrenyl acetal, methacrylate acetal, styrenyl ether, or methacrylate moiety resulting in four different monomers. These monomers were then separately polymerized using free radical polymerization with azobisisobutyronitrile (AIBN) as an initiator to synthesize the glycopolymers. ...
Citation Formats
T. E. L. Douglas et al., “Enzymatic Mineralization of Hydrogels for Bone Tissue Engineering by Incorporation of Alkaline Phosphatase,” MACROMOLECULAR BIOSCIENCE, pp. 1077–1089, 2012, Accessed: 00, 2020. [Online]. Available: https://hdl.handle.net/11511/68588.