Enzymatic Mineralization of Hydrogels for Bone Tissue Engineering by Incorporation of Alkaline Phosphatase

Douglas, Timothy E. L.
Messersmith, Philip B.
Chasan, Safak
Mikos, Antonios G.
de Mulder, Eric L. W.
Dickson, Glenn
Schaubroeck, David
Balcaen, Lieve
Vanhaecke, Frank
Dubruel, Peter
Jansen, John A.
Leeuwenburgh, Sander C. G.
Alkaline phosphatase (ALP), an enzyme involved in mineralization of bone, is incorporated into three hydrogel biomaterials to induce their mineralization with calcium phosphate (CaP). These are collagen type I, a mussel-protein-inspired adhesive consisting of PEG substituted with catechol groups, cPEG, and the PEG/fumaric acid copolymer OPF. After incubation in Ca-GP solution, FTIR, EDS, SEM, XRD, SAED, ICP-OES, and von Kossa staining confirm CaP formation. The amount of mineral formed decreases in the order cPEG?>?collagen?>?OPF. The mineral:polymer ratio decreases in the order collagen?>?cPEG?>?OPF. Mineralization increases Young's modulus, most profoundly for cPEG. Such enzymatically mineralized hydrogel/CaP composites may find application as bone regeneration materials.


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Douglas, Timothy E. L.; Gassling, Volker; Declercq, Heidi A.; Purcz, Nicolai; Pamula, Elzbieta; Haugen, Havard J.; Chasan, Safak; de Mulder, Eric L. W.; Jansen, John A.; Leeuwenburgh, Sander C. G. (Wiley, 2012-05-01)
Membranes of the autologous blood-derived biomaterial platelet-rich fibrin (PRF) were functionalized by incorporation of alkaline phosphatase (ALP), an enzyme involved in mineralization of bone, and subsequently incubated in calcium glycerophosphate (CaGP) solution to induce PRFs mineralization with calcium phosphate (CaP) to improve PRFs suitability as a material for bone replacement. Incorporated ALP retained its bioactivity and induced formation of CaP material within PRF membranes, as confirmed by SEM, ...
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K5-type yeast killer toxin secreted by P. anomala NCYC 434 cells has a broad killing spectrum. Competitive inhibiton of killer activity showed that glucans, mainly the beta-1,3 glucan, represent the primary toxin binding site within the cell wall of sensitive cells. Its hydrolytic activity on laminarin in an exo-like fashion revealed that the toxin exerts its killing effect by exo-beta-1,3-glucanase activity. Its specific activity on laminarin was 120U/mg, and the Michaelis constants K-m, and V-max for lami...
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Semi-empirical molecular orbital treatment at the level of AM1 type has been performed on Huckel type silacyclacenes having arenoid rings (R) of 2-9. The first and second type cryptoannulenic effects are defined.
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Calcium carbonate (CaCO3) microspheres consisting of vaterite polymorph have been widely used in biomedical applications. Specifically, vaterite microspheres having hollow cores showed significant potential in drug delivery, however the spontaneous transformation of vaterite to other polymorphs in aqueous environments reduced its controlled in vivo release capability. In this work, calcite and aragonite microspheres having hollow/porous inner cores were synthesized -for the first time-using sodium dodecyl s...
Citation Formats
T. E. L. Douglas et al., “Enzymatic Mineralization of Hydrogels for Bone Tissue Engineering by Incorporation of Alkaline Phosphatase,” MACROMOLECULAR BIOSCIENCE, pp. 1077–1089, 2012, Accessed: 00, 2020. [Online]. Available: https://hdl.handle.net/11511/68588.