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Enzymatic Mineralization of Hydrogels for Bone Tissue Engineering by Incorporation of Alkaline Phosphatase
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Date
2012-08-01
Author
Douglas, Timothy E. L.
Messersmith, Philip B.
Chasan, Safak
Mikos, Antonios G.
de Mulder, Eric L. W.
Dickson, Glenn
Schaubroeck, David
Balcaen, Lieve
Vanhaecke, Frank
Dubruel, Peter
Jansen, John A.
Leeuwenburgh, Sander C. G.
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Alkaline phosphatase (ALP), an enzyme involved in mineralization of bone, is incorporated into three hydrogel biomaterials to induce their mineralization with calcium phosphate (CaP). These are collagen type I, a mussel-protein-inspired adhesive consisting of PEG substituted with catechol groups, cPEG, and the PEG/fumaric acid copolymer OPF. After incubation in Ca-GP solution, FTIR, EDS, SEM, XRD, SAED, ICP-OES, and von Kossa staining confirm CaP formation. The amount of mineral formed decreases in the order cPEG?>?collagen?>?OPF. The mineral:polymer ratio decreases in the order collagen?>?cPEG?>?OPF. Mineralization increases Young's modulus, most profoundly for cPEG. Such enzymatically mineralized hydrogel/CaP composites may find application as bone regeneration materials.
Subject Keywords
Biotechnology
,
Materials Chemistry
,
Bioengineering
,
Polymers and Plastics
,
Biomaterials
URI
https://hdl.handle.net/11511/68588
Journal
MACROMOLECULAR BIOSCIENCE
DOI
https://doi.org/10.1002/mabi.201100501
Collections
Department of Biology, Article
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T. E. L. Douglas et al., “Enzymatic Mineralization of Hydrogels for Bone Tissue Engineering by Incorporation of Alkaline Phosphatase,”
MACROMOLECULAR BIOSCIENCE
, pp. 1077–1089, 2012, Accessed: 00, 2020. [Online]. Available: https://hdl.handle.net/11511/68588.