Enzymatic Mineralization of Hydrogels for Bone Tissue Engineering by Incorporation of Alkaline Phosphatase

Download

Date

2012-08-01

Author

Douglas, Timothy E. L.
Messersmith, Philip B.
Chasan, Safak
Mikos, Antonios G.
de Mulder, Eric L. W.
Dickson, Glenn
Schaubroeck, David
Balcaen, Lieve
Vanhaecke, Frank
Dubruel, Peter
Jansen, John A.
Leeuwenburgh, Sander C. G.

Metadata

Show full item record

This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

Item Usage Stats

346
views

0
downloads

Alkaline phosphatase (ALP), an enzyme involved in mineralization of bone, is incorporated into three hydrogel biomaterials to induce their mineralization with calcium phosphate (CaP). These are collagen type I, a mussel-protein-inspired adhesive consisting of PEG substituted with catechol groups, cPEG, and the PEG/fumaric acid copolymer OPF. After incubation in Ca-GP solution, FTIR, EDS, SEM, XRD, SAED, ICP-OES, and von Kossa staining confirm CaP formation. The amount of mineral formed decreases in the order cPEG?>?collagen?>?OPF. The mineral:polymer ratio decreases in the order collagen?>?cPEG?>?OPF. Mineralization increases Young's modulus, most profoundly for cPEG. Such enzymatically mineralized hydrogel/CaP composites may find application as bone regeneration materials.

Subject Keywords

Biotechnology, Materials Chemistry, Bioengineering, Polymers and Plastics, Biomaterials

URI

https://hdl.handle.net/11511/68588

Journal

MACROMOLECULAR BIOSCIENCE

DOI

https://doi.org/10.1002/mabi.201100501

Collections

Department of Biology, Article

Suggestions

OpenMETU
Core

Enzymatically induced mineralization of platelet-rich fibrin Douglas, Timothy E. L.; Gassling, Volker; Declercq, Heidi A.; Purcz, Nicolai; Pamula, Elzbieta; Haugen, Havard J.; Chasan, Safak; de Mulder, Eric L. W.; Jansen, John A.; Leeuwenburgh, Sander C. G. (Wiley, 2012-05-01) Membranes of the autologous blood-derived biomaterial platelet-rich fibrin (PRF) were functionalized by incorporation of alkaline phosphatase (ALP), an enzyme involved in mineralization of bone, and subsequently incubated in calcium glycerophosphate (CaGP) solution to induce PRFs mineralization with calcium phosphate (CaP) to improve PRFs suitability as a material for bone replacement. Incorporated ALP retained its bioactivity and induced formation of CaP material within PRF membranes, as confirmed by SEM, ...
Enzymic activity of the K5-type yeast killer toxin and its characterization Izgu, F; Altinbay, D; Sertkaya, A (Informa UK Limited, 2005-11-01) K5-type yeast killer toxin secreted by P. anomala NCYC 434 cells has a broad killing spectrum. Competitive inhibiton of killer activity showed that glucans, mainly the beta-1,3 glucan, represent the primary toxin binding site within the cell wall of sensitive cells. Its hydrolytic activity on laminarin in an exo-like fashion revealed that the toxin exerts its killing effect by exo-beta-1,3-glucanase activity. Its specific activity on laminarin was 120U/mg, and the Michaelis constants K-m, and V-max for lami...
Immobilization of invertase and glucose oxidase in poly 2-methylbutyl-2-(3-thienyl) acetate/polypyrrole matrices Isik, S; Alkan, S; Toppare, Levent Kamil; Cianga, I; Yagci, Y (Elsevier BV, 2003-12-01) Immobilization of invertase and glucose oxidase in conducting polypyrrole and copolymers of poly 2-methylbutyl-2-(3-thienyl) acetate with pyrrole were achieved via electrochemical method. Sodium dodecyl sulphate was found to be the most suitable supporting electrolyte. Maximum reaction rate, Michaelis-Menten constant and optimum temperatures were determined for native and immobilized enzymes. Storage and operational stabilities of enzyme electrodes were also investigated.
Poly(ethylene terephthalate)/Carbon Nanotube Composites Prepared With Chemically Treated Carbon Nanotubes Yesil, Sertan; Bayram, Göknur (Wiley, 2011-07-01) Surfaces of multiwalled carbon nanotubes (CNT) were functionalized by treatment with strong acid mixture (purification) followed by modification with sodium dodecyl sulfate, poly(ethylene glycol) (PEG), and diglycidyl ether of Bisphenol A (DGEBA). Poly(ethylene terephthalate) (PET)-based conductive polymer composites were prepared by using these CNT by means of melt mixing with a twin screw extruder. Amount of carboxylic acid groups on the CNT surface increased after acid treatment but decreased with surfac...
Immobilization of cholesterol oxidase in a conducting copolymer of thiophene-3-yl acetic acid cholesteryl ester with pyrrole Çırpan, Ali; Toppare, Levent Kamil; YAGCI, Y (Informa UK Limited, 2003-01-01) Cholesterol oxidase has been immobilized in conducting copolymers of thiophene-3-yl acetic acid cholesteryl ester with pyrrole (CM/PPy) and polypyrrole (PPy) via electropolymerization. p-Toluene sulphonic acid was used as the supporting electrolyte. Kinetic parameters (V-max and K-m) and operational stability of enzyme electrodes were investigated. Surface morphology of the films was examined by scanning electron microscope.

Citation Formats

T. E. L. Douglas et al., “Enzymatic Mineralization of Hydrogels for Bone Tissue Engineering by Incorporation of Alkaline Phosphatase,” MACROMOLECULAR BIOSCIENCE, pp. 1077–1089, 2012, Accessed: 00, 2020. [Online]. Available: https://hdl.handle.net/11511/68588.