Investigation of wheat genes involved in zinc efficiency mechanism using differential display technique

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2003

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Türktaş, Mine

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Zinc is a metal involved in structure of many enzymes, in the growth and differentiation of plants. Wheat is one of the most consumed cereals. Some wheat cultivars can̕t deal with zinc deficiency and this situation not only reduces grain yield but also weakens the resistance of cereals to diseases and impairs the nutritional quality of the grain. Some wheat cultivars are not affected by zinc deficiency. In this study, ءdifferential display̕, used for determination differentially expressed genes between two samples, was performed. The most zinc efficient bread wheat cultivar Kiraç-66 was grown in hydroponics medium and samples were taken at different time periods. RNA isolations were done and differential display technique was performed. After examining the results, differentially expressed bands were selected and sequenced. DNA sequence analysis were done in available databases which showed that three of the bands were fragments of putative zinc transporters. In this study we have found threee putative gene fragments using differential display technique on zinc efficient plants grown under differeing zinc concentrations. These fragments showed homology with zinc transporter, ABC transporter and ADH (Alcohol Dehydrogenase). It is known that all of these three genes are involved in zinc efficiency mechanism. Further studies will be conducted on these gene fragments.

Subject Keywords

Zinc efficieny, Zinc deficiency, Differential display, Bread wheat, Hydroponics, ABC transporter, Zinc transporter, Alcohol dehydrogenase

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http://etd.lib.metu.edu.tr/upload/1259860/index.pdf
https://hdl.handle.net/11511/13634

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Graduate School of Natural and Applied Sciences, Thesis

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Citation Formats

M. Türktaş, “Investigation of wheat genes involved in zinc efficiency mechanism using differential display technique,” M.S. - Master of Science, Middle East Technical University, 2003.