Trehalose metabolism in wheat and identification of trehalose metabolizing enzymes under abiotic stress conditions

El-Bashiti, Tarek
Trehalose (a-D-glucopyranosyl-1,1-a-D-glucopyranoside) is a non reducing disaccharide of glucose that occurs in a large variety of organisms, ranging from bacteria to invertebrate animals, where it serves as an energy source or stress protectant. Until recently, only few plant species, mainly desiccation tolerant ءresurrection̕ plants, were considered to synthesize trehalose. Although most plant species do not appear to accumulate easily detectable amounts of trehalose, the discovery of genes for trehalose biosynthesis in Arabidopsis and in a range of crop plants suggests that the ability to synthesize trehalose is widely distributed in the plant kingdom. In this study, three wheat cultivars (Triticum aestivum L.) Tosun, Bolal (stress tolerant) and Çakmak (stress sensitive) were analysed for the presence of trehalose. Using gas chromatography-mass spectrometry (GC-MS) analysis, trehalose was unambiguously identified in extracts from seeds and seedlings of three different wheat cultivars (Bolal, Tosun and Çakmak). The trehalose amount was quantified by high performance liquid chromatography connected with refractory index detector. Effects of drought and salt stress on trehalose contents of wheat cultivars were studied at seedling level and trehalose analysis was achieved both on shoot and root tissues. It was found that trehalose had accumulated under salt and drought stress conditions in all wheat cultivars. The highest trehalose accumulation was detected in roots of Bolal cultivar under drought stress condition. Furthermore, trehalose metabolizing enzymes; trehalose-6-phosphate synthase (TPS) and trehalase enzyme activities were measured in roots and shoots of Bolal and Çakmak cultivars under control, salt and drought stress conditions. The most interesting results that we found that TPS activity sharply increased under stress conditions. The activity of TPS in


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Many studies have described the physiological, biochemical and molecular responses to boron (B) toxicity and deficiency individually in plants. However, overall pattern of gene expression changes and their physiological and biochemical significances after high B, low B and supplementing artificial B-enriched fertilizer (Tarımbor) application have not been proposed up till now. In the present study, we assessed global changes in gene expression of two wheat cultivars Bolal-2973 and Atay-85 differing in their ...
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Wheat, like other cereal species, shows a common recalcitrance and strong genotypic variation in tissue culture and Agrobacteriummediated transformation. A variety of explant sources have been used for obtaining wheat plants from in vitro cultures and Agrobacterium- mediated transformation. These are: isolated microspores (Mejza et al., 1993), mature embryos (Özgen et al., 1998; Delporte et al., 2001), immature embryos (Ozias-Akins and Vasil, 1982; Vasil et al., 1990; Pellegrineschi et al., 2004), and immat...
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It is important to understand the underlying plant defense mechanisms in order to establish best strategies to reduce losses due to diseases in cereals. The current available information is mostly on model organisms and their plant-pathogen interactions. However, this study is focused on the identification of genes involved in the resistance mechanism of one of the most devastating diseases of wheat, yellow rust. The strategy undertaken was to use differential display method (DD) together with microarray te...
Trehalose Glycopolymers as Excipients for Protein Stabilization
Lee, Juneyoung; Lin, En-Wei; Lau, Uland Y.; Hedrick, James L.; Bat, Erhan; Maynard, Heather D. (American Chemical Society (ACS), 2013-08-01)
Herein, the synthesis of four different trehalose glycopolymers and investigation of their ability to stabilize proteins to heat and lyophilization stress are described. The disaccharide, alpha,alpha-trehalose, was modified with a styrenyl acetal, methacrylate acetal, styrenyl ether, or methacrylate moiety resulting in four different monomers. These monomers were then separately polymerized using free radical polymerization with azobisisobutyronitrile (AIBN) as an initiator to synthesize the glycopolymers. ...
Citation Formats
T. El-Bashiti, “Trehalose metabolism in wheat and identification of trehalose metabolizing enzymes under abiotic stress conditions,” Ph.D. - Doctoral Program, Middle East Technical University, 2003.