Optimisation of tissue culture, regeneration and Agrobacterium-mediated transformation paramaters in winter wheat cultivars (T.durum cv. Kiziltan-91 and T.aestivum cv. Bezostaja-01)

Kavas, M.
Öktem, Hüseyin Avni
Wheat, like other cereal species, shows a common recalcitrance and strong genotypic variation in tissue culture and Agrobacteriummediated transformation. A variety of explant sources have been used for obtaining wheat plants from in vitro cultures and Agrobacterium- mediated transformation. These are: isolated microspores (Mejza et al., 1993), mature embryos (Özgen et al., 1998; Delporte et al., 2001), immature embryos (Ozias-Akins and Vasil, 1982; Vasil et al., 1990; Pellegrineschi et al., 2004), and immature inflorescences (Ozias-Akins and Vasil,1982; He and Lazzeri, 2001). Also there are different target tissues for Agrobacterium-mediated transformation for wheat. Immature inflorescences have been reported as an alternative source of explant for stable transformation of durum wheat (He and Lazzeri, 1998) and bread wheat (Rasco-Gaunt and Barcelo, 1999). This explant has also shown good expression of T-DNA delivered genes after Agrobacterium co-cultivation (Amoah et al., 2001). Advantages of the immature inflorescence system are that they are easier to isolate and are harvested from much younger plants allowing more efficient use of space in donor-plant growth facilities. The objective of the present study was to investigate the influence of vernalization period of donor plant and callus age on regeneration capacity of calli, number of tillers, number of seeds and Agrobacterium-mediated transformation efficiency produced from immature inflorescence cultures of two winter wheat cultivars.
11th Annual Congress of the International- Association-for-Plant-Tissue-Culture-and-Biotechnology


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Citation Formats
M. Kavas, H. A. Öktem, and M. YÜCEL, “Optimisation of tissue culture, regeneration and Agrobacterium-mediated transformation paramaters in winter wheat cultivars (T.durum cv. Kiziltan-91 and T.aestivum cv. Bezostaja-01),” presented at the 11th Annual Congress of the International- Association-for-Plant-Tissue-Culture-and-Biotechnology, Beijing, PEOPLES R CHINA, 2006, Accessed: 00, 2020. [Online]. Available: https://hdl.handle.net/11511/39676.