Biochemical monitoring of toxic and carcinogenic organic pollutants along the İzmir Bay after the great canal project and possible health effects

Download

index.pdf

Date

2004

Author

Boyuneğmez, Tuğba

Metadata

Show full item record

Item Usage Stats

236
views

109
downloads

The induction of hepatic cytochrome P4501A1 and its monooxygenase activity 7-ethoxyresorufin O- deethylase, (EROD) in fish by PAHs, PCBs and dioxins has been suggested as an early warning system أmost sensitive biochemical responseؤ for assessing environmental contamination conditions. In this study, the degree of induction of cytochrome P4501A1 protein as determined immunochemically and CYP1A1 associated EROD activity in fish were utilized as biomarkers of exposure to PAHs, PCBs and related organic pollutants along the Izmir Bay on the Aegean Sea Coast after the Great Canal Project. Three different fish species were used throughout this study, namely leaping mullet (Liza saliens), annular seabream (Diplodus annularis) and common sole (Solea vulgaris) which were representatives of pelagic, benthopelagic and benthic fish, respectively. Fish were sampled in November 2002 and October 2003 from different sites of the Bay. The mullet caught from Harbor, Üçkuyular port site, and Pasaport region displayed highly elevated EROD activities which were 2258±840 (n=15), 2011±490 (n=4), 1813±287 (n=11) pmole /min/mg protein, respectively and were 104, 80 and 79 fold higher than that of fish obtained from the reference point (25±9 pmole/min/mg protein; n=4). Mullet caught along the pollutant gradient at three other sites (Hekim Island, Inciralti, and Zeytinalani) exhibited less but highly significant induced EROD activity. EROD activities of common sole sampled from Foça open sites (107±20 pmol/min/mg protein, n=5) and site16A (80±12 pmol/min/mg protein, n=9) were found to be very low and the latter was accepted as reference site. The highest EROD activity were seen in fish captured from Inciralti which was about 6.3 times higher than those obtained from reference site. Common sole caught from the mouth of Gediz River and Hekim Island exhibited also highly elevated EROD

Subject Keywords

General Biology.

URI

http://etd.lib.metu.edu.tr/upload/12604746/index.pdf
https://hdl.handle.net/11511/14032

Collections

Graduate School of Natural and Applied Sciences, Thesis

Suggestions

OpenMETU
Core

In vitro induction of growth and development of common juniper(Juniperus communis L.) from shoot and bud explants Koçer, Zeynep Ahsen; Kaya, Zeki; Department of Biotechnology (2005) The objective of the study was to investigate the optimum conditions for in vitro regeneration of common juniper (Juniperus communis L.) by using indirect organogenesis approach. Throughout the study; callus induction, organogenesis, improved organogenesis and root induction experiments were performed sequentially. It was found that explant position, genotype, gender, treatments and sampling time had significant effects on callus induction rate in common juniper. The results of treatments indicated that IBA...
Preliminary approch for the determination of fish exuded kairomone using fourier transform infrared spectroscopy Kepenek, Ayşe Özge; Severcan, Feride; Department of Biology (2005) Chemical communication in aquatic organisms has been topic of a large number of studies focusing interactions between organisms via info chemicals. Diel Vertical Migration (DVM) is commonly observed among zooplankton and consists of a single daily ascent with minimum depth reached between sunset and sunrise and a descent with maximum depth attained during the day. DVM was absent or reduced when predators were absent and well developed in their presence. Species of the Daphnia are one of the well investigate...
Hydrogen production by microorganisms in solar bioreactor Uyar, Başar; Eroğlu, İnci; Department of Biotechnology (2008) The main objective of this study is exploring the parameters affecting photobiological hydrogen production and developing anaerobic photobioreactor for efficient photofermentative hydrogen production from organic acids in outdoor conditions. Rhodobacter capsulatus and Rhodobacter sphaeroides strains were used as microorganisms. EU project “Hyvolution” targets to combine thermophilic fermentation with photofermentation for the conversion of biomass to hydrogen. In this study, the effluent obtained by dark fe...
Antimicrobial spectrum determination of the K5 type yeast killer protein on bacteria causing skin infections and its cell killing activity Gönen, Tuğçe; İzgü, Kadri Fatih; Department of Biology (2006) Some yeast strains secrete extracellular polypeptide toxins known to have potential growth inhibitory activity on sensitive yeast cells. These yeast strains are known as killer yeasts and their toxins are named as killer toxins or killer proteins. Yeast killer proteins are found inhibitory to Gram-positive bacteria in several studies which were based on microbial interactions of the producer strains tested with sensitive strains. K5 type yeast killer protein produced by Pichia anomala NCYC 434 was previousl...
Determination of antimicrobial spectrum of K9 type yeast killer toxin and its cell killing activity Yener, Burcu; İzgü, Kadri Fatih; Department of Biology (2006) Some yeast strains secrete extracellular polypeptide toxins known to have potential growth inhibitory activity on other sensitive yeast genera but are immune to their own toxins. These yeast strains are termed as killer yeasts and their toxins are designated as killer proteins or killer toxins. Killer phenotypes are classified into 11 typical types (K1-K11). The toxic actions of yeast killer proteins on sensitive cells show differences and one of the most important toxic actions involves the selective funct...

Citation Formats

T. Boyuneğmez, “Biochemical monitoring of toxic and carcinogenic organic pollutants along the İzmir Bay after the great canal project and possible health effects,” M.S. - Master of Science, Middle East Technical University, 2004.