Extension of flower longevity in transgenic plants via antisense blockage of ethylene bıosynthesis

Deçani Yol, Betul
Ethylene (C2H4) is a very simple molecule, a gas, and has numerous effects on the growth, development and storage life of many fruits, vegetables and ornamental crops. In higher plants, ethylene is produced from L-methionine in essentially all tissues and ACC Synthase and ACC Oxidase are the two key enzymes in the biosynthesis of ethylene. The objective of the present study was to transform tobacco (Nicotiana tabacum L. cv. Samsun) plant with partial sequence of torenia acc oxidase gene in antisense and sense orientations via Agrobacterium-mediated gene transfer system, and to analyze its effect on ethylene production in transgenic plants. Six antisense and seven sense T0 putative transgenic lines were obtained and were further analyzed with several assays. Leaf disc assay and chlorophenol red assay under selection (75 mg/L kanamycin) revealed positive results compared to the non-transformed plant. T1 generations were obtained from all putative transgenic lines. PCR analysis and Northern Blot Hybridization results confirmed the transgenic nature of T1 progeny. Furthermore, ethylene amount produced by flowers were measured with gas chromatography, which resulted in an average of 77% reduction in S7 line and 72% reduction in A1 line compared with the control flowers. These results indicated that, transgenic tobacco plants carrying torenia acc oxidase transgene both in antisense and sense orientations showed reduced ethylene production thus a possibility of flower life extension.


Optimization of regeneration and agrobacterium mediated transformation of sugar beet (Beta vulgaris L.)
Baloğlu, Mehmet Cengiz; Yücel, Ayşe Meral; Department of Biology (2005)
In this study, optimization of a transformation and regeneration system via indirect and direct organogenesis in cotyledon, hypocotyl, petiole, leaf and shoot base tissues of sugar beet (Beta vulgaris L. cv. ELK 345 and 1195) was investigated. Two different germination, three different callus induction and shoot induction medium was used for indirect organogenesis of sugar beet cultivar ELK 345. Except cotyledon, other explants (hypocotyl, petiole and leaf) produced callus. However no shoot development was ...
Identification and cloning of genes induced and / or repressed upon treatments of wheat plants (avocet s) with BTH, BABA and Trichoderma Harzianum Raifi KRL-AG2
Al-Asbahi, Adnan; Akkaya, Mahinur S.; Department of Biotechnology (2006)
One of the major problems concerning the production of food crops is the controlling of plant diseases to maintain the high quality and yield. Wheat diseases are caused by parasitic bacteria, fungi and viruses that are a major hazard in wheat production. Therefore, understanding of any resistance mechanism is prerequisite for the successful utilization of wheat crop species in modern agriculture. The phenomenon of induced resistance by fungi, bacteria, microbial elicitors and chemicals has been investigated...
Effect of boron and iron stress on phytosiderophore production in barley
İnce, Yetkin Çaka; Yücel, Ayşe Meral; Department of Biology (2015)
Boron and iron are essential nutrients for growth and development of higher plants. Deficiency and toxicity of them cause significant changes in physiological, cellular and molecular make-up of plants. Iron uptake in barley is mainly achieved by production and release of phytosiderophores to the environment. On the other hand, production of phytosiderophores are affected by concentrations of boron and iron. Four enzymes which function in phytosiderophore hydroxymugineic acid (HMA) production namely NAS, NAA...
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Kavas, Musa; Yücel, Ayşe Meral; Öktem, Hüseyin Avni; Department of Biology (2011)
Soil salinity negatively affects agricultural production in Turkey by decreasing the yield and quality. Direct introduction of stress related genes by genetic engineering is one of the most rapid approaches to develop stress tolerant crops. In this study, TaNHX1 gene was isolated from bread wheat and three different local wheat cultivars were transformed with overexpression vectors containing TaNHX1 gene by using Agrobacterium-mediated and particle bombardment gene transfer techniques. Immature embryo and i...
Improvement of Pectinase Production by Co-culture of Bacillus spp. Using Apple Pomace as a Carbon Source
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Increasing interest in using food and agricultural wastes for low cost enzyme production continues in fermentation industry. Bacteria, especially Bacillus subtilis, are known to produce pectinases in synthetic media and media amended with agricultural wastes as a carbon source, whereas the potential of co-culturing Bacillus species to produce pectinases has not been fully studied yet. In this study, co-culture of B. subtilis and B. pumilus was optimized for solid load and pH to maximize pectinase production...
Citation Formats
B. Deçani Yol, “Extension of flower longevity in transgenic plants via antisense blockage of ethylene bıosynthesis,” M.S. - Master of Science, Middle East Technical University, 2004.