Extension of flower longevity in transgenic plants via antisense blockage of ethylene bıosynthesis

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2004

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Deçani Yol, Betul

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Ethylene (C2H4) is a very simple molecule, a gas, and has numerous effects on the growth, development and storage life of many fruits, vegetables and ornamental crops. In higher plants, ethylene is produced from L-methionine in essentially all tissues and ACC Synthase and ACC Oxidase are the two key enzymes in the biosynthesis of ethylene. The objective of the present study was to transform tobacco (Nicotiana tabacum L. cv. Samsun) plant with partial sequence of torenia acc oxidase gene in antisense and sense orientations via Agrobacterium-mediated gene transfer system, and to analyze its effect on ethylene production in transgenic plants. Six antisense and seven sense T0 putative transgenic lines were obtained and were further analyzed with several assays. Leaf disc assay and chlorophenol red assay under selection (75 mg/L kanamycin) revealed positive results compared to the non-transformed plant. T1 generations were obtained from all putative transgenic lines. PCR analysis and Northern Blot Hybridization results confirmed the transgenic nature of T1 progeny. Furthermore, ethylene amount produced by flowers were measured with gas chromatography, which resulted in an average of 77% reduction in S7 line and 72% reduction in A1 line compared with the control flowers. These results indicated that, transgenic tobacco plants carrying torenia acc oxidase transgene both in antisense and sense orientations showed reduced ethylene production thus a possibility of flower life extension.

Subject Keywords

Plant Culture.

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http://etd.lib.metu.edu.tr/upload/3/12605135/index.pdf
https://hdl.handle.net/11511/14307

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Graduate School of Natural and Applied Sciences, Thesis

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Citation Formats

B. Deçani Yol, “Extension of flower longevity in transgenic plants via antisense blockage of ethylene bıosynthesis,” M.S. - Master of Science, Middle East Technical University, 2004.