Fluorescence determination of monosaccharides and catecholamines by using dansylaminophenyl boronic acid

Seçkin, Z. Ebru
In sugar industry, determination of invert sugars (fructose and glucose) provides information about the quality, process control and characteristics of the produced fructose and glucose syrups. Determination of invert sugar is also important for wine industries. In wine process, fructose and glucose are converted to ethanol by fermentation and the type of wine is designated by the amount of invert sugar remained. Fast and reliable invert sugar detection techniques are required to check the quality of wine throughout the fermentation process. Cathecholamines (eg. dopamine and epinephrine) are diol containing compounds which play important roles in higher animals̕ physicomotor activities, learning, sleeping, memory and immune system. They also affect brain functions. Inbalances in dopamine level in brain result in a number of psychiatric disorders, particularly schizophrenia and Parkinson disease. Catecholamines are present in relatively high amounts in drugs. Many efforts have been made to develop analytical procedures for their rapid, simple and accurate determination. For these reasons, catecholamine quantification is important in the field of pharmacy and medicine. Boronic acids interact with 1,2 or 1,3-diol containing compounds, such as fructose, glucose and dopamine, rapidly and reversibly. Hence, boronic acids are used as the recognition moeity in the construction of photoinduced electron transfer (PET) fluorophores specific for saccharides and catecholamines. In this study, a flow injection analysis system has been developed for the rapid and selective recognition of fructose and glucose in wine and in commercial sugar syrups; dopamine and epinephrine in pharmaceutical injections by using dansylaminophenyl boronic acid (DAPB) which is a fluorescent PET molecule.


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Immobilization of fluorescent chemosensors and chromogenic reagents on solid supports for developing optical sensors result in improved analytical performance characteristics such as continuous read-out, increased sensitivity, lower reagent consumption and possibility of using the sensor in solvents where the free molecule displays low solubility. The aim of this study is to immobilize dansylaminophenyl boronic acid (DAPB acid) and diphenylcarbazide (DPC) into various solid supports for the determination of...
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Lactic acid is a fermentation-derived organic acid used in a wide range of industries, such as food processing and pharmaceuticals. Its market is expected to expand due to the worldwide concern for the environment, as it is an essential feedstock for biodegradable polymers. However, fermentation product is a very dilute, multicomponent aqueous solution. Subsequent separation, purification and concentration of organic acids is difficult because of high affinity of the acids for water. Reactive extraction is ...
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An improved analytical method which offers rapid, accurate determination and identification of 22 amino acids in a variety of matrices, e.g. baby foods, juices, honey is reported. The amino acids were extracted from the matrixes using acidified water. Simultaneous determination of 22 underivatized amino acids was carried out by a liquid chromatography-mass spectrometry (LC/MS). A narrow-bore column allowed rapid screening and quantitative analysis by positive LC/atmospheric pressure chemical ionization (APC...
ERGUCYENER, C; AYGUN, S; Ataman, Osman Yavuz; TEMIZER, A (1988-01-01)
Mercury speciation in aqueous solutions containing inorganic mercury and methylmercury has been demonstrated using electrochemical separation prior to determination by cold-vapour atomic absorption spectrometry. Approximately 95% of the inorganic mercury was electro-deposited on a Pt electrode at –0.1 V, whereas for methylmercury –0.2 V or even more negative potentials were required. Organic mercury was determined by NaBH4 reduction after electrochemical separation. Inorganic mercury was determined in the o...
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One of the advantages of the ETV sample introduction is the ability to temporally separate analyte elements in complex mixtures by differences in their vaporization temperatures within the ETV for ICPMS. However, the broadening of the transient peaks in the transport tubing often obscures this temporal resolution. This study shows that decreasing the transport tubing diameter produces little broadening beyond that produced during aerosol production in the ETV. Maintaining such narrow peaks through the trans...
Citation Formats
Z. E. Seçkin, “Fluorescence determination of monosaccharides and catecholamines by using dansylaminophenyl boronic acid,” M.S. - Master of Science, Middle East Technical University, 2004.