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Fluorescence determination of monosaccharides and catecholamines by using dansylaminophenyl boronic acid

Seçkin, Z. Ebru
In sugar industry, determination of invert sugars (fructose and glucose) provides information about the quality, process control and characteristics of the produced fructose and glucose syrups. Determination of invert sugar is also important for wine industries. In wine process, fructose and glucose are converted to ethanol by fermentation and the type of wine is designated by the amount of invert sugar remained. Fast and reliable invert sugar detection techniques are required to check the quality of wine throughout the fermentation process. Cathecholamines (eg. dopamine and epinephrine) are diol containing compounds which play important roles in higher animals̕ physicomotor activities, learning, sleeping, memory and immune system. They also affect brain functions. Inbalances in dopamine level in brain result in a number of psychiatric disorders, particularly schizophrenia and Parkinson disease. Catecholamines are present in relatively high amounts in drugs. Many efforts have been made to develop analytical procedures for their rapid, simple and accurate determination. For these reasons, catecholamine quantification is important in the field of pharmacy and medicine. Boronic acids interact with 1,2 or 1,3-diol containing compounds, such as fructose, glucose and dopamine, rapidly and reversibly. Hence, boronic acids are used as the recognition moeity in the construction of photoinduced electron transfer (PET) fluorophores specific for saccharides and catecholamines. In this study, a flow injection analysis system has been developed for the rapid and selective recognition of fructose and glucose in wine and in commercial sugar syrups; dopamine and epinephrine in pharmaceutical injections by using dansylaminophenyl boronic acid (DAPB) which is a fluorescent PET molecule.