Detection of genetically modified potatoes by the polymerase chain reaction

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2005

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Muwonge, Abubaker

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Quite a number of important crops have been genetically modified with genes for agronomically important traits, such as insect and viral resistance. As the numbers of genetically modified foods continue to increase on the market, the need for rapid development of GMO detection methods is indispensable. This study was carried out to detect if genetically modified potatoes exist on food market in Turkey. Thirty samples from different places were collected. Using a DNA based PCR method, potato samples were examined for the presence of 35S promoter, Nos terminator, neomycin phosphotransferase (nptII) genes, and synthetic cry3A gene which is the general transgene in all approved Newleaf transgenic potato lines. The experimental design of this study was to detect Newleaf insect resistant lines. In 11 samples at least one genetic element was detected. Sample R from Ankara has shown to be belonging to Newleaf insect resistant lines. Since 35S promoter was not detected in samples M3, 14 and F1, it is proposed that they are belonging to Newleaf virus and insect resistant lines (Newleaf plus or Newleaf Y). Although Nos terminator was not detected in samples H2, Z2 and D, cry3A fragments amplified in those samples have been verified that they are from the synthetic cry3A regions of Newleaf lines. The detected synthetic cry3A gene in GM potatoes was amplified by specific primers, which cannot amplify Bacillus thuringiensis tenebrionis natural cry3A gene. In addition, the authenticity of the synthetic cry3A PCR products were confirmed by both sequencing and restriction digestions. Our results showed that genetically modified Newleaf potatoes exist in food market in Turkey. Further studies by accredited laboratories are strongly recommended.

Subject Keywords

Pest Control and Treatment of Diseases., Plant Protection.

URI

http://etd.lib.metu.edu.tr/upload/12605783/index.pdf
https://hdl.handle.net/11511/14991

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Graduate School of Natural and Applied Sciences, Thesis

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Citation Formats

A. Muwonge, “Detection of genetically modified potatoes by the polymerase chain reaction,” M.S. - Master of Science, Middle East Technical University, 2005.