Preparation and characterization of poly(d,l-lactide-co-glycolide) microspheres for controlled release of anticancer drugs

Download

index.pdf

Date

2005

Author

Eyövge, Gökçen

Metadata

Show full item record

Item Usage Stats

242
views

101
downloads

Breast cancer is the most frequent type of cancer seen in woman. Chemotherapy is one of the most important treatments for breast cancer. However, systemic toxicity, drug resistance and unstable kinetics of the drug in the blood are serious problems of chemotherapy. The use of biodegradable polymers for controlled release of anticancer drugs has gained popularity in recent years. Controlled release of anticancer drugs from polymeric carriers has some advantages such as improvement in the efficiency of treatment, reduction in systemic toxicity and prevention of the drug resistance that is developed by the cancer cells. In this study, it was aimed to prepare such a controlled release system for anticancer drugs which are used in breast cancer treatment by using biodegradable copolymer poly(D,L-lactide-co-glycolide) and to characterize in terms of morphology, size, drug content and drug release rate. In the first part of this study; empty and drug loaded poly (D,L-lactide-co-glycolide) microspheres were prepared. Two sets of empty poly(D,L-lactide-co-glycolide) microspheres were prepared by solvent evaporation technique with single emulsion (oil/water) to determine the effect of stirring rate on size of microspheres. Increase in stirring rate caused decrease in size of microspheres. Drug loaded poly(D,L-lactide-co-glycolide) microspheres were prepared for controlled release of anticancer drugs which are used in breast cancer treatment namely; 5-fluorouracil, methotrexate and tamoxifen by using solvent evaporation technique either with double emulsion (water/oil/water) or single emulsion (oil/water). In the second part of this study; empty and drug loaded microspheres were characterized. Inverted light microscopy and scanning electron microscopy were used to examine morphology and size of microspheres. Drug content of microspheres and amount of released drug were

Subject Keywords

General Biology.

URI

http://etd.lib.metu.edu.tr/upload/3/12606382/index.pdf
https://hdl.handle.net/11511/15276

Collections

Graduate School of Natural and Applied Sciences, Thesis

Suggestions

OpenMETU
Core

N-acetyl transferase (NAT1&NAT2) and glutathione-S transferase (GSTM1&GSTT1) genetic polymorphisms in breast cancer Atalay, Ayçin; Güray, Tülin; Department of Biology (2006) Breast cancer is the most frequent malignancy among women, especially in Western societies. Highly penetrant genes such as BRCA1 and BRCA2, together with the reproductive history can constitute only 30% of the cause, so there should be other common genes, which may play a role in breast carcinogenesis according to one's lifestyle. In our case, the effect of N-acetyl transferases (NAT1, NAT2) and glutathione-S transferases (GSTM1&GSTT1) were investigated, since variations in these genes may alter their enzym...
Preparation of polyethylene glycol coated magnetic nanoparticles for targeting of cancer cells Keskin, Tuğba; Gündüz, Ufuk; Department of Biology (2012) Conventional cancer chemotherapies cannot differentiate between healthy and cancer cells, and lead to severe side effects and systemic toxicity. In the last decades, different kinds of controlled drug delivery systems have been developed to overcome these shortcomings of chemotherapeutics. Magnetic nanoparticles (MNP) are potentially important in cancer treatment since they can be targeted to tumor site by an externally applied magnetic field. In this study, it is aimed to synthesize folic acid conjugated; ...
Comparison of the Diagnostic Accuracy of Next Generation Sequencing and Microarray Resequencing Methods for Detection of BRCA1 and BRCA2 Gene Mutations Bahsi, Taha; Ergun, Sezen Güntekin; Ergun, Mehmet Ali; Perçin, E. Ferda (Gazi University, 2018-4-1) Objective: Breast cancer constitutes 29 % of estimated new cases of cancer in women, and it is also one of the major cause of death in all cancer types. In this study, DNA samples of familial breast cancer patients with BRCA1 and BRCA2 mutations which had been analyzed using conventional DNA sequencing method, were also analyzed with new methods including microarray and next generation sequencing (NGS) in order to compare their results Methods: Seven patients with BRCA1 mutation, one patient with BRCA2 m...
Detection of bladder tumor recurrence by fourier transform infrared spectroscopy as a novel method Aydın, Özge Zelal; Severcan, Feride; Department of Biology (2009) Bladder cancer is one of the most common urogenital cancers worldwide. Two techniques commonly used for bladder cancer diagnosis are urine cytology and cystoscopy. Cytology is not sensitive for detecting tumors. Cystoscopy is an invasive technique which disturbs patient comfort. In the current study, we used Fourier transform infrared (FT-IR) spectroscopy as a novel method which is rapid and non-invasive to investigate the bladder tumor recurrence using the bladder wash samples collected in the course of co...
Application of image enhancement algorithms to improve the visibility and classification of microcalcifications in mammograms Akbay, Cansu; Gençer, Nevzat Güneri; Gençer, Gülay; Department of Biomedical Engineering (2015) Breast cancer is the second leading cause of cancer deaths for women. Mammography is the most effective technology presently available for breast cancer screening, despite the fact that there are still some limitations of the imaging technique, such as insufficient resolution, low local contrast and noise combined with the subtle nature of the usual radiographic findings. One of the most important radiographic findings associated to the existence of breast cancer is the clustered microcalcifications. Especi...

Citation Formats

G. Eyövge, “Preparation and characterization of poly(d,l-lactide-co-glycolide) microspheres for controlled release of anticancer drugs,” M.S. - Master of Science, Middle East Technical University, 2005.