Detection of bladder tumor recurrence by fourier transform infrared spectroscopy as a novel method

Download

index.pdf

Date

2009

Author

Aydın, Özge Zelal

Metadata

Show full item record

Item Usage Stats

243
views

104
downloads

Bladder cancer is one of the most common urogenital cancers worldwide. Two techniques commonly used for bladder cancer diagnosis are urine cytology and cystoscopy. Cytology is not sensitive for detecting tumors. Cystoscopy is an invasive technique which disturbs patient comfort. In the current study, we used Fourier transform infrared (FT-IR) spectroscopy as a novel method which is rapid and non-invasive to investigate the bladder tumor recurrence using the bladder wash samples collected in the course of control cystoscopy. This study is unique since it is the first one to use the bladder wash sample in the diagnosis of the bladder tumor by using FT-IR spectroscopy. Molecular investigation of the FT-IR spectra revealed many differences between control and tumor samples such as a considerable increase in protein, carbohydrate and nucleic acids content, and changes in protein and carbohydrate structure. On the basis of the spectral differences, cluster analysis was performed to differentiate between the control and tumorous spectra and we reached to an overall sensitivity (including all individuals with tumor) of 91.8%, a PUNLMP sensitivity of 83.3% and a papilloma sensitivity of 77.8% in spectral range 1444-1457 cm-1. Other spectral ranges also gave similar results. Our results showed that FT-IR spectroscopy can be used to detect the bladder tumors in bladder wash sample with higher sensitivity compared to cytology. In summary, we propose the utilization of the FT-IR spectroscopy for the detection of bladder tumors since specific spectral regions might be used as effective markers for the diagnosis.

Subject Keywords

Biology., Research

URI

http://etd.lib.metu.edu.tr/upload/2/12610883/index.pdf
https://hdl.handle.net/11511/18870

Collections

Graduate School of Natural and Applied Sciences, Thesis

Suggestions

OpenMETU
Core

Preparation of polyethylene glycol coated magnetic nanoparticles for targeting of cancer cells Keskin, Tuğba; Gündüz, Ufuk; Department of Biology (2012) Conventional cancer chemotherapies cannot differentiate between healthy and cancer cells, and lead to severe side effects and systemic toxicity. In the last decades, different kinds of controlled drug delivery systems have been developed to overcome these shortcomings of chemotherapeutics. Magnetic nanoparticles (MNP) are potentially important in cancer treatment since they can be targeted to tumor site by an externally applied magnetic field. In this study, it is aimed to synthesize folic acid conjugated; ...
Preparation and characterization of poly(d,l-lactide-co-glycolide) microspheres for controlled release of anticancer drugs Eyövge, Gökçen; Gündüz, Ufuk; Department of Biotechnology (2005) Breast cancer is the most frequent type of cancer seen in woman. Chemotherapy is one of the most important treatments for breast cancer. However, systemic toxicity, drug resistance and unstable kinetics of the drug in the blood are serious problems of chemotherapy. The use of biodegradable polymers for controlled release of anticancer drugs has gained popularity in recent years. Controlled release of anticancer drugs from polymeric carriers has some advantages such as improvement in the efficiency of treatm...
Relationship between the nat genetic polymorphism and susceptibility to prostate cancer Dilek, Derya; Güray, Tülin; Department of Biology (2008) Prostate cancer (PCa) is one of the most prevelant cancers in males in many countries, increasing in frequency with age. PCa incidence and mortality rates are not evenly distributed worldwide. Family history is an established risk factor for prostate cancer and families demonstrating autosomal dominant or X-linked transmission of susceptibility have been observed. Although an increasing number of candidate genes or hereditary prostate cancer susceptibility have been identified, only 5 to 10 percent of prost...
Identification of rare cell types in lung cancer and investigation of their use in prognosis Yıldız, Cemal; Şengül Babal, Simge; Pir, Pınar (Orta Doğu Teknik Üniversitesi Enformatik Enstitüsü; 2022-10) Cancer is a complex, dynamic and heterogeneous disease. Identification of tumor heterogeneity holds a significant promise not only on tumor classification but also on predicting the prognosis and choosing the right treatment. Therefore, it is essential to investigate tumor heterogeneity in a regular diagnosis procedure. Tumors contain variety of cell types such as malignant cells, non-malignant cells, immune cells, and rare cells (i.e., dormant cells (DRM) and cancer stem cells (CSC))....
Cell-surface interactions in a breast cancer model Antmen, Ezgi; Hasırcı, Vasıf Nejat; Demirci, Utkan; Department of Biotechnology (2017) Breast cancer, is one of the most commonly diagnosed cancers, has a high mortality rate. One in every eight women (12.3%) develops breast cancer at some stage of their lives and this is the cause of about 15% of cancer deaths in women and 3% of total deaths. It is therefore important to study the behavior of breast cancer cells. Measurement of the mechanical properties of cancer cells leads to new insights such as that cancer cells are softer than healthy cells. Also, metastatic cancer cells were found to b...

Citation Formats

Ö. Z. Aydın, “Detection of bladder tumor recurrence by fourier transform infrared spectroscopy as a novel method,” M.S. - Master of Science, Middle East Technical University, 2009.