Immobilization of tyrosinase in polysiloxane/polypyrrole copolymer matrices

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2006

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Arslan, Ahu

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Immobilization of tyrosinase in conducting copolymer matrices of pyrrole functionalized polydimethylsiloxane/polypyrrole (PDMS/PPy) were achieved by electrochemical polymerization. The polysiloxane/polypyrrole/tyrosinase electrode was constructed by the entrapment of enzyme in conducting matrices during electrochemical copolymerization. Maximum reaction rate (Vmax) and Michaelis-Menten constant (Km) were investigated for immobilized enzyme. Enzyme electrodes were prepared in two different electrolyte/solvent systems. The effect of supporting electrolytes, p-toluene sulfonic acid and sodium dodecyl sulfate on the enzyme activity and film morphology were determined. Temperature and pH optimization, operational stability and shelf-life of enzyme electrodes were also examined. Phenolic contents of green and black tea were determined by using enzyme electrodes.

Subject Keywords

Polymers.

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http://etd.lib.metu.edu.tr/upload/12606988/index.pdf
https://hdl.handle.net/11511/15681

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Graduate School of Natural and Applied Sciences, Thesis

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Citation Formats

A. Arslan, “Immobilization of tyrosinase in polysiloxane/polypyrrole copolymer matrices,” M.S. - Master of Science, Middle East Technical University, 2006.