Investigation of telomerase activity in diagnosis of endometrial and cervical cancer

Eskiocak, Uğur
Human telomerase is a ribonucleoprotein complex that adds hexameric TTAGGG repeats to the ends of chromosomes in order to prevent their shortening. Telomerase activity has been evaluated for its diagnostic and prognostic value in cancer since it is observed in most malignancies but not in most normal somatic tissues. In this study telomerase activity was examined in tumor specimens obtained from cervix, endometrium and their non-cancerous regions by an improved telomeric repeat amplification protocol (TRAP) silver staining assay. Appearance of characteristic TRAP leader with 6 base pair increments indicate a positive result and was observed in all cancerous and some of the non-cancerous tissues. Telomerase activities of carcinoma tissues and normal counterparts were compared by densitometric analysis after PCR. Significantly higher telomerase activity was observed in cervical carcinoma samples compared to normal adjacent tissue. No significant difference was observed between endometrium carcinomas and normal endometrial tissue in terms of telomerase activity. High telomerase activity in normal endometrium restricts the use of assay for detection of carcinogenesis. However, in cervical tissues an accurate quantification of telomerase activity by TRAP silver stain assay may be valuable as a confirmatory assay.


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İzgi, Ahu; Gündüz, Ufuk; Department of Biology (2012)
Human telomerase is a reverse transcriptase which synthesizes telomeric repeat sequences at the ends of chromosomes. The telomerase enzyme has two essential subunits to be functional which are called telomerase reverse transcriptase (hTERT) and human telomerase RNA (hTR). Telomerase uses its RNA subunit as a template for the addition of hexameric repeats at the ends of chromosomes. The activity of telomerase has been detected in immortal cells but not in most normal somatic cells. Therefore, its activity co...
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The nucleosome is the repeating unit of chromatin and it is composed of an octamer histone core that holds together around 145 base pairs of DNA in a disc-shaped configuration. In higher eukaryotes, an additional histone, known as linker histone H1, provides further structural order by stabilizing the nucleosomal DNA termini. Histone H1 family includes seven somatic variants (H1.0 to H1.5 and H1.10) and four variants expressed only in germ cells, three of which are testisspecific (H1T, H1T2m and HILS1), and...
Intergenic and Repeat Transcription in Human, Chimpanzee and Macaque Brains Measured by RNA-Seq
Xu, Augix Guohua; He, Liu; Li, Zhongshan; Xu, Ying; Li, Mingfeng; Fu, Xing; Yan, Zheng; Yuan, Yuan; Menzel, Corinna; Li, Na; Somel, Mehmet; Hu, Hao; Chen, Wei; Paabo, Svante; Khaitovich, Philipp (Public Library of Science (PLoS), 2010-07-01)
Transcription is the first step connecting genetic information with an organism's phenotype. While expression of annotated genes in the human brain has been characterized extensively, our knowledge about the scope and the conservation of transcripts located outside of the known genes' boundaries is limited. Here, we use high-throughput transcriptome sequencing (RNA-Seq) to characterize the total non-ribosomal transcriptome of human, chimpanzee, and rhesus macaque brain. In all species, only 20-28% of non-ri...
Comparative study of three simple molecular approaches in search of mtDNA haplogroup identification of domestic sheep
Yuncu, Eren; Demirci, Sevgin; Bastanlar, Evren Koban; Dogan, Sukru Anil; Tasdemir, Umut; Togan, İnci Zehra (Elsevier BV, 2013-08-01)
There are five (A-E) identified mitochondria, DNA (mtDNA) haplogroups (HPGs) in domestic sheep. In search of a quick and reliable HPG classification, three different molecular approaches were tested: the restriction fragment length polymorphism (RFLP) method applied to mtDNA control region (CR), the single strand conformational polymorphism (SSCP) method applied to NADH dehydrogenase subunit 2 (MT-ND2) and to NADH dehydrogenase subunit 4 (MT-ND4) regions. The results were evaluated comparatively to elucidat...
Harnessing DNA intercalation
Persil Çetinkol, Özgül (2007-10-01)
Numerous small molecules are known to bind to DNA through base pair intercalation. Fluorescent dyes commonly used for nucleic acid staining, such as ethidium, are familiar examples. Biological and physical studies of DNA intercalation have historically been motivated by mutation and drug discovery research. However, this same mode of binding is now being harnessed for the creation of novel molecular assemblies. Recent studies have used DNA scaffolds and intercalators to construct supramolecular assemblies t...
Citation Formats
U. Eskiocak, “Investigation of telomerase activity in diagnosis of endometrial and cervical cancer,” M.S. - Master of Science, Middle East Technical University, 2007.