The effect of indole acetic acid, abscisic acid, gibberellin and kinetin on the expression of arf1 gtp binding protein of pea (pisum sativum l. cv. araka)

Ertekin, Özlem
ADP Ribosylation Factor 1 (ARF1) is a universal small GTP binding protein which has an important role in vesicular trafficking between endoplasmic reticulum and Golgi. ARF1 is a basic component of Coat Protein I (COPI) vesicles which have functions in both formation of coatomer complex and recruitment of cargo proteins. In this study, the expression ARF1 was analyzed in pea (P. sativum L. cv. Araka) grown at different developmental stages. Because of the differential hormonal levels at corresponding stages, the effects of hormones on ARF1 expression were also studied. The results of present research show that ARF1 expression in embryos and 2 days grown plants after germination is lower when compared to 6 days grown plants. In order to see the hormonal effect, 3 weeks old plants were supplied with 50M of each hormone for 3 times on alternate days. Protein extraction, cell fractionation,Western blot was carried out and immunoblot analysis was conducted with AtARF1 polyclonal antibodies. It was shown that, in pea shoots, abscisic acid and gibberellin increases the inactive GDP bound ARF1 by hydrolyzing ARF-GTP through activating ARFGTPase activating protein (ARF-GAP) or partially inhibiting ARF-Guanine Nucleotide Exchange Factor (ARF-GEF). In roots, ARF-GDP (cytosolic fraction), ARF-GTP (microsomal fraction) and total amount of ARF1 (13.000 x g supernatant fraction) were down regulated by ~11, ~19 and ~11 fold respectively with the application of gibberellin; and by ~11, ~7 and ~3 fold respectively with the application of abscisic acid; when compared to control plants. These results indicate the importance of plant hormones in the regulation of ARF1 in pea. ms in the future.


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Citation Formats
Ö. Ertekin, “The effect of indole acetic acid, abscisic acid, gibberellin and kinetin on the expression of arf1 gtp binding protein of pea (pisum sativum l. cv. araka),” M.S. - Master of Science, Middle East Technical University, 2007.