The effect of indole acetic acid, abscisic acid, gibberellin and kinetin on the expression of arf1 gtp binding protein of pea (pisum sativum l. cv. araka)

Ertekin, Özlem
ADP Ribosylation Factor 1 (ARF1) is a universal small GTP binding protein which has an important role in vesicular trafficking between endoplasmic reticulum and Golgi. ARF1 is a basic component of Coat Protein I (COPI) vesicles which have functions in both formation of coatomer complex and recruitment of cargo proteins. In this study, the expression ARF1 was analyzed in pea (P. sativum L. cv. Araka) grown at different developmental stages. Because of the differential hormonal levels at corresponding stages, the effects of hormones on ARF1 expression were also studied. The results of present research show that ARF1 expression in embryos and 2 days grown plants after germination is lower when compared to 6 days grown plants. In order to see the hormonal effect, 3 weeks old plants were supplied with 50M of each hormone for 3 times on alternate days. Protein extraction, cell fractionation,Western blot was carried out and immunoblot analysis was conducted with AtARF1 polyclonal antibodies. It was shown that, in pea shoots, abscisic acid and gibberellin increases the inactive GDP bound ARF1 by hydrolyzing ARF-GTP through activating ARFGTPase activating protein (ARF-GAP) or partially inhibiting ARF-Guanine Nucleotide Exchange Factor (ARF-GEF). In roots, ARF-GDP (cytosolic fraction), ARF-GTP (microsomal fraction) and total amount of ARF1 (13.000 x g supernatant fraction) were down regulated by ~11, ~19 and ~11 fold respectively with the application of gibberellin; and by ~11, ~7 and ~3 fold respectively with the application of abscisic acid; when compared to control plants. These results indicate the importance of plant hormones in the regulation of ARF1 in pea. ms in the future.


The effects of insertional mutations in comQ, comP, srfA, spo0H, spo0A and abrB genes on bacilysin biosynthesis in Bacillus subtilis
Karatas, AY; Cetin, S; Özcengiz, Gülay (Elsevier BV, 2003-04-15)
In Bacillus subtilis, two extracellular signaling peptides, ComX pheromone and CSF (competence and sporulation factor), stimulate the development of genetic competence and surfaction biosynthesis in response to high cell density (quorum sensing) by regulating the activity of transcription factor ComA. We recently showed that biosynthesis of dipeptide antibiotic bacilysin is linked to ComA and PhrC(CSF) in a Spo0K(Opp)-dependent manner by constructing phrC-, comA- and oppA-disrupted mutants of B. subtilis. I...
YURDUGÜL, SEYHUN; Pancevska, Natalija-Atanasova; YILDIZ, GÜLGEZ GÖKÇE; Bozoglu, Faruk (2012-01-01)
The digestive system of the ruminants possesses anaerobic fungi, which are responsible for the degradation of cellulose and cell wall structures by their enzyme systems. An enzyme complex, bearing cellulase activity from Neocallimastix spp. which belongs to anaerobic rumen fungi, was partially isolated and its effect at various concentrations on bread quality was tested. The addition of enzyme complex into bread dough resulted in a decrease of hardness, gumminess and chewiness, providing a softer crumb, ind...
SENER, E; TURGUT, H; YALCIN, I; OREN, I; Türker, Burhan Lemi; CELEBI, N; AKIN, A (Elsevier BV, 1994-09-19)
The synthesis and in vitro antimicrobial activity of 5-substituted 2-(3-pyridyl)benzoxazoles (3-7) is described for some Gram-positive and Gram-negative bacteria and the yeast Candida albicans. The compounds 3-7 exhibited significant activity against the screened microorganisms, having MIC values between 25 and 12.5 mu g/ml. Quantum-chemical calculations were performed for the compounds 1-10, in order to observe some feasible structure-activity relationships. These theoretical observations, calculated for t...
The effect of cysteine-43 mutation on thermostability and kinetic properties of citrate synthase from Thermoplasma acidophilum
Kocabıyık, Semra; Russel, RJM; Danson, MJ; Hough, DW (Elsevier BV, 1996-07-05)
In this study, we have substituted serine-43 by cysteine in the recombinant citrate synthase from a moderately thermophilic Archaeon Thermoplasma acidophilum, for site-specific attachment of labels and have investigated the effects of this mutation on the biochemical properties and thermal stability of the enzyme. Both wild-type and the mutant enzymes were purified to homogenity using affinity chromatography on Matrex Gel Red A. The mutant Thermoplasma citrate synthase is very similar to wild-type citrate s...
The immunological co-adjuvant action of liposomal interleukin-2: The role of mode of localisation of the cytokine and antigen in the vesicles
Gürsel, Mayda (Informa UK Limited, 1998-01-01)
In experiments designed to study the co-adjuvant action of interleukin-2, a model antigen (tetanus toroid) was passively entrapped in, or covalently coupled to multilamellar liposomes in the presence or absence of interleukin-2 (IL-2). When present, IL-2 was either co-entrapped with the toroid, entrapped alone in liposomes with toroid coupled to their surface, or coupled to the surface of liposomes with entrapped toroid. The role of spatial localization of IL-2 within the liposomal structure (vis a vis that...
Citation Formats
Ö. Ertekin, “The effect of indole acetic acid, abscisic acid, gibberellin and kinetin on the expression of arf1 gtp binding protein of pea (pisum sativum l. cv. araka),” M.S. - Master of Science, Middle East Technical University, 2007.