Preparation of sericin based wound dressing and investigation of its biomaterial properties

Download
2009
Aktürk, Ömer
In this study, it was aimed to produce sericine/collagen composite membranes and to investigate their properties as a wound dressing. Different membrane compositions were prepared by casting and solvent evaporation method. After initial studies for optimization of ratios, membrane groups at two different thicknesses were prepared for each selected ratio and cross-linked with 3 % (w/v) glutaraldehyde (GTA). Considering the wound dressing requirements, equilibrium degree of swelling (EDS), water vapor transmission rate (WVTR), oxygen permeability, mechanical properties, in situ degradation, microbial penetration and cytotoxicity of membranes were examined. The EDS of membranes had a range of 14.91 to 4.37 (g/g) and increased significantly with the presence of sericin. There was no obvious relationship between the sericin ratio of membranes and WVTR, but the increase in membrane thickness decreased WVTR significantly. Thin and sericin containing membranes had statistically better oxygen permeabilities. Sericin deteriorated the tensile strength and elongation of membranes statistically. Cross-linked groups were resistant to hydrolytic degradation through 4 weeks of incubations. None of the membranes were penetrable to bacteria owing to their dense structure. For cytotoxicity studies, 3T3 fibroblasts and keratinocytes were seeded on membranes separately, and analyzed with MTT assays, and light microscopy and scanning electron microscopy (SEM). As regards to MTT assay, keratinocytes proliferated significantly on membranes and reached to high confluence within 7 days. Similarly, fibroblasts also showed high proliferation on membranes. Light microscopy and SEM analysis showed that both cells could attach, grow and spread on membranes. Also, cells gained their characteristic morphology after 1 day and formed flattened structure within 7 days.

Suggestions

Preparation and Characterization of Antibacterial Zeolite-Polyurethane Composites
Kamisoglu, K.; Çatman Aksoy, Emine; Akata Kurç, Burcu; Hasırcı, Nesrin; BAÇ, NURCAN (2008-12-05)
In this study, antibacterial properties were induced in polyurethane (PU) films by the incorporation of Ag+-loaded zeolites as fillers. The mechanical and thermal properties of the prepared composites were also investigated. For this purpose, two different types of zeolite microparticles, high-silica zeolite beta crystals and low-silica zeolite A crystals, were synthesized. Ag+ was added by ion exchange, and these particles were incorporated into the PU prepolymer, which was prepared from its main component...
Fabrication and characterization of bilayered tissue scaffolds incorporating bioactive agents for skin tissue engineering applications
Aktürk, Ömer; Keskin, Dilek; Bilici, Temel; Department of Engineering Sciences (2015)
In this study, it was aimed to fabricate tissue scaffolds from different biological polymers (collagen, silk fibroin and sericin) for skin tissue engineering applications. For this purpose, bilayered scaffolds composed of epidermal (collagen/sericin films) and dermal (collagen sponges, collagen matrices or silk fibroin matrices) layers were produced with different biomaterial fabrication methods. Casting and solvent evaporation (film), lyophilization/freeze-drying (sponge) and dry/wet electro-spinning (micr...
Analysis of Antimicrobial and Coagulation Inducing Effects of a New Decontamination Powder
Tosun, Barış Çağrı; Öktem, Hüseyin Avni (2019-11-27)
In this study, the effect of a new formulation in the form of powder for the purpose of decontamination of skin from biological agents is investigated. Moreover, the formulation has also been shown to induce coagulation by erythrocyte aggregation, which is of significance since decontamination against B agents and bleeding wounds could be dealt with simultaneously by applying single formulation.
Collagen/gold nanoparticle nanocomposites: A potential skin wound healing biomaterial
Akturk, Omer; Kismet, Kemal; Yasti, Ahmet C.; Kuru, Serdar; Duymus, Mehmet E.; Kaya, Feridun; Caydere, Muzaffer; Hucumenoglu, Sema; Keskin, Dilek (2016-08-01)
In this study, nanocomposite collagen scaffolds incorporating gold nanoparticles (AuNPs) were prepared for wound healing applications. Initially, dose (<20ppm) and size (>20nm) of AuNPs that were not cytotoxic on HaCat keratinocytes and 3T3 fibroblasts were determined. Both collagen sponges and AuNP-incorporated nanocomposites (CS-Au) were cross-linked with glutaraldehyde (CS-X and CS-AuX). Incorporation of AuNPs into cross-linked scaffolds enhanced their stability against enzymatic degradation and increase...
Improving the physical properties of fish gelatin by high hydrostatic pressure (HHP) and ultrasonication (US)
Sezer, Purlen; Okur, İlhami; Öztop, Halil Mecit; Alpas, Hami (2020-04-01)
In this study, it was aimed to improve the physical properties of fish gelatin by using high hydrostatic pressure (HHP) and ultrasonication (US). Gelatin solutions were exposed to different pressures and ultrasonication separately and gelled afterwards. The physicochemical measurements based on gel strength, turbidity and rheology experiments showed that HHP treatment on fish and bovine gelatin stabilized the gelatin network by organising the structure and reducing the free volume. Both processing methods (...
Citation Formats
Ö. Aktürk, “Preparation of sericin based wound dressing and investigation of its biomaterial properties,” M.S. - Master of Science, Middle East Technical University, 2009.