Stem cell based nerve tissue engineering on guided constructs

Download

index.pdf

Date

2009

Author

Yücel, Deniz

Metadata

Show full item record

Item Usage Stats

283
views

106
downloads

Nerve injury is a serious clinical problem that has a direct impact on the quality of life. Nerve tissue engineering (NTE) is one of the most promising methods in human health care to restore the function of damaged neural tissues. The current state of the art involves the construction of a tissue engineered, nano or micropatterned 3-D nerve tube that has fibers or channels in the inside. The scope of this study is to construct a 3-D, biodegradable nerve tube which consists of an aligned, electrospun mat seeded with stem cells that is wrapped in a porous micropatterned film which contains support cells. In two separate approaches human mesenchymal stem cells (MSCs) and mouse neural stem cells (NSCs) were used. In the design with the MSCs, the micropatterned exterior part of the nerve tube contained undifferentiated MSCs as support cells and this was wrapped around the fibers seeded with MSCs which were induced to neural differentiation. In the other case, NSCs differentiated into astrocytes were used as support cells seeded on the micropatterned film and the mat was loaded with undifferentiated NSCs. Differentiation into neural cells and astrocytes were shown with immunocytochemistry and RT-PCR. The neuron-like MSCs and NSCs were shown to express neural marker β-Tubulin III whereas astrocytes expressed glial fibrillary acidic protein (GFAP), an astrocyte marker. RT-PCR showed that early neural markers, nestin and Nurr 1, were expressed at passage 4 by undifferentiated MSCs and by MSCs induced to neural differentiation, while these markers were not expressed in undifferentiated MSCs at passages 2 and 3. The cells aligned along the axis of the micropattern of the film and along the axis of the fiber on the fibrous mat. This behavior was also maintained after construct formation. MTS and confocal microscopy revealed that the cells were viable and homogeneously distributed over the two parts of the scaffold. This indicates that the construct has a potential to be tested in vivo for nerve tissue engineering purposes.

Subject Keywords

Biology., Biotechnology.

URI

http://etd.lib.metu.edu.tr/upload/3/12610399/index.pdf
https://hdl.handle.net/11511/18474

Collections

Graduate School of Natural and Applied Sciences, Thesis

Suggestions

OpenMETU
Core

Apolipoprotein a5 genetic polymorphisms in turkish population and the risk of ischemic stroke Şahin, Esra; Adalı, Orhan; Department of Biotechnology (2008) Stroke is the third leading cause of death and the most common cause of disabilities worldwide. Apolipoprotein A5 gene (APO A5), which encodes a 369 amino acid protein called Apolipoprotein AV (apo AV), has several single nucleotide polymorphisms (SNPs) found to be associated with altered triglyceride (TG) levels. Atherosclerosis is a major cause of ischemic stroke and this pathology may be associated with variability of TG levels. The main objective of this study was to investigate the coding region (c.553...
Etiology of senile osteoporosis - A hypothesis Atik, O. Sahap; Uslu, M. Murad; Eksioglu, Fatih; Satana, Tolgay (Ovid Technologies (Wolters Kluwer Health), 2006-02-01) Osteoporosis is a major health problem characterized by compromised bone strength predisposing patients to an increased risk of fracture. It may cause morbidity and mortality in elderly men and women. The etiologic factors that lead to senile osteoporosis still are unclear.
Analysis of whiplash during rear crash and development of an anti-whiplash seat mechanism Özdemir, Mustafa; İder, Sıtkı Kemal; Gökler, Mustafa İlhan; Department of Mechanical Engineering (2013) Whiplash injury is one of the most important types of injuries when its resulting longterm pain is considered. This neck injury is frequently seen in the motor vehicle collisions, especially in the low-speed rear collisions. In this thesis, it is aimed to analyze the whiplash syndrome in low-speed rear-end impacts and develop an antiwhiplash seat mechanism that reduces the risk of occurrence of whiplash injuries. Firstly, using the commercially available finite element model of the BioRID II dummy, backset ...
Tissue Engineered, Guided Nerve Tube Consisting of Aligned Neural Stem Cells and Astrocytes Yucel, Deniz; Kose, Gamze Torun; Hasırcı, Vasıf Nejat (2010-12-01) Injury of the nervous system, particularly in the spinal cord, impairs the quality of life of the patient by resulting in permanent loss of neurologic function. The main limitation in spinal cord regeneration is the lack of extracellular matrix to guide nerves for functional recovery of the transected nerve tissue. In the present study, a tissue engineered nerve tube was prepared by wrapping neural stem cells (NSCs) on aligned fibers using a micropatterned film with astrocytes aligned along the microgrooves...
Lanthanum doped dicalcium phosphate bone cements for potential use as filler for bone defects Motameni, Ali; Alshemary, Ammar Z.; Dalgıç, Ali Deniz; Keskin, Dilek; Evis, Zafer (2021-03-01) The bone defects arising as a result of trauma should be filled to provide a framework to support and encourage the growth of new and living bone tissues. Among the many synthetic bone graft substitutes, self-hardening calcium phosphate (CP) cements have been widely used to repair hard tissue defects. In this study, pure dical-cium phosphate (DCP) and lanthanum (La) modified dicalcium phosphate (La-DCP) bone cements were prepared based on acid/base reaction between beta-tricalcium phosphate (beta TCP) (or L...

Citation Formats

D. Yücel, “Stem cell based nerve tissue engineering on guided constructs,” Ph.D. - Doctoral Program, Middle East Technical University, 2009.