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Determination of polymorphism of pgm, hk, pgi, and g6pd in different developmental stages of honey bee (apis mellifera l.) and its relation with pgm activity and glycogen content
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Date
2010
Author
Yeni, Filiz
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In this study, three subspecies of Apis mellifera L. (A. m. caucasica, A. m. carnica, and A. m. syriaca) from different climatic regions were evaluated electrophoretically at ontogenetic level by means of four enzymes, namely Phosphoglucomutase (PGM), Hexokinase (HK), Phosphoglucose isomerase (PGI) and Glukose-6-phosphate dehydrogenase (G6PD). It is determined that only Pgm and Hk loci were polymorphic. Allele and genotype frequencies at Pgm locus changes seasonally whereas Hk locus does not exhibit seasonal variation. Within the scope of this study we investigated at which developmental stage shifting to heterozygotes prior to winter occurs. It is found that there is a seasonal fluctuation throughout the year in Pgm genotype frequencies at each developmental stage studied and correlated with enzyme activity and glycogen content. As the studied enzymes have crucial v roles in insect energy metabolism, results of this study provided further information about the relationship between carbohydrate metabolism and enzyme polymorphism of honey bees.
Subject Keywords
Biology.
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http://etd.lib.metu.edu.tr/upload/2/12611930/index.pdf
https://hdl.handle.net/11511/19660
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Graduate School of Natural and Applied Sciences, Thesis
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F. Yeni, “Determination of polymorphism of pgm, hk, pgi, and g6pd in different developmental stages of honey bee (apis mellifera l.) and its relation with pgm activity and glycogen content,” M.S. - Master of Science, Middle East Technical University, 2010.
