Molecular phylogenetic position of Turkish abies (pinaceae) based on noncoding trn regions of chloroplast genome

Özdemir Değirmenci, Funda
Abies is the second largest genus of family Pinaceae (after Pinus), consisting of about 51 species, all native to the Northern Hemisphere. There are six native taxa belonging to this genus growing in pure and mixed stands in Turkey. Abies cilicica subsp. isaurica, Abies nordmanniana subsp. bornmülleriana, Abies nordmanniana subsp. equi-trojani, Abies x olcayana are endemic and considered as lower risk (LR) species. To determine the genetic relationships in Turkish firs, 18 populations of different subspecies of Abies were collected from different regions of Turkey and non-coding trn regions of chloroplast DNA were sequenced to assess the genetic structure of the studied species. trnL, trnF and trnV region were examined. All the trn regions of Abies species in the world (aproximately 300 species that found in the IPNI (The International Plant Names Index) were investigated in the database of NCBI. The available trn sequences of 23 Abies species worldwide included into the analyses. All analyses to estimate molecular diversity parameters were carried out with the MEGA software. The constructed phylogenetic tree with the trn sequences revealed that Turkish firs formed a monophyletic group with almost no sequence divergence. v Since sequence data for all three sectors of trn were not available from the NCBI data base, the phylogentic analysis with the sequence data of trnL regions were compartively analyzed in all firs. The results showed that Turkish- European species formed a single clade, which clearly differentiated them from the others, such as Japanese species, A. veitchii. Similarly, according to the sequence data of trnF, Turkish fir species were grouped together and distinctly separated from Asian-American Fir species. The results suggest that all Turkish firs may have evolved from single ancestral fir species, most likely from Abies nordmanniana.
Citation Formats
F. Özdemir Değirmenci, “Molecular phylogenetic position of Turkish abies (pinaceae) based on noncoding trn regions of chloroplast genome,” M.S. - Master of Science, Middle East Technical University, 2011.