Date

2012

Author

Aras, Ebru

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Obesity becomes an epidemic health problem in developing and developed countries, which arises due to stable life style and increase in the consumption of high fat diets. Obesity is generally accompanied with various clinical disorders, such as insulin resistance, type II diabetes, hypertension, dyslipidemia and cardiovascular diseases. This study aims to characterize and quantify different lipid classes in longissimus dorsi (LD) and quadriceps (Q) skeletal muscles of control (DBA/2J), obese Berlin fat mouse inbred (BFMI) and Berlin muscle mouse inbred (BMMI) lines, which display high fat and high muscle content, respectively. These mouse lines were special due to their phenotypes, especially BFMI lines, which displayed spontaneous and strong obesity. These lines, more specifically BFMI860 and BFMI861, were also special due to their possibility of being an animal model of cardiovascular diseases and metabolic syndrome, since they also displayed insulin resistance. For separation,identification and quantification of various lipids of these lines, a novel method was developed which gives better separation of main lipid classes via using high performance liquid chromatography (HPLC) coupled to an evaporative light scattering detector (ELSD). Addition of triethylamine (TEA) to the solvents being used, and altering the parameters of HPLC and ELSD instruments, and also the gradient elution, provided a better separation with an enhanced resolution. This method has been applied to the lipid extracts obtained from longissimus dorsi (LD) and quadriceps (Q) skeletal muscles of control (DBA/2J), obese Berlin fat mouse inbred (BFMI) and Berlin muscle mouse inbred (BMMI). In this method, a binary gradient elution composed of n-Hexane, isopropanol, methanol, acetic acid and triethylamine was applied to the samples. All interested lipid classes, namely triglyceride (TG), cholesteryl ester (CO), cholesterol (C), 1-oleoyl-rac-glycerol (MG), phosphatidylcholine (PC) and cardiolipin (CLPN), all of which have been known to have a role in obesity, insulin resistance, and cardiovascular diseases, were separated, identified and quantified via this novel method. According to the results, among BFMI lines, BFMI860 and BFMI861 lines and BMMI806, among BMMI lines, are worth to study obesity. Especially, the former ones may also become animal models for cardiovascular diseases and metabolic syndrome.

Subject Keywords

Muscles., Lipids., Obesity., Fourier transform infrared spectroscopy., Mice as laboratory animals.

URI

http://etd.lib.metu.edu.tr/upload/12614568/index.pdf
https://hdl.handle.net/11511/21721

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Graduate School of Natural and Applied Sciences, Thesis