Determination of inbred obese mouse adipose tissue lipid profiles by high performance liquid chromatography

Başer, Tuğçe
Obesity becomes an important and serious health problem all over the world. Consumed too much calorie and less physical activity are the major issues in obesity. Obesity also causes some other important diseases such as hypertension, insulin resistance, dyslipidemia, type II diabetes and cardiovascular diseases. The aim of this study is to identify and quantify lipid profiles of obese Berlin Fat Mouse Inbred (BFMI) which has high fat content. These mouse lines showed spontaneous obesity. In addition to obesity, BFMI 860 and 861 had insulin resistance. Also, BFMI 860 and 861 lines were used as animal model for cardiovascular diseases. High performance liquid chromatography (HPLC) was used to separate, identify and quantify the lipids of BFMI mouse. Evaporative light scattering detector (ELSD) was used as detector with HPLC. Lipid extraction procedures were applied to BFMI. After that, HPLC were done with a gradient elution. Lipids that were identified and quantified were triglyceride (TG), diglyceride (DG), cholesterol (C), monoglyceride (MG) and phosphatidylcholin (PC). It is known that all of these lipids have role in cardiovascular diseases, insulin resistance and obesity. With respect to results, BFMI 860 and 861 mouse lines have more lipids especially TG, C, and MG than BFMI 852 and BFMI 856. Therefore, these lines are suitable for usage as animal models for obesity and type II diabetes.
Citation Formats
T. Başer, “Determination of inbred obese mouse adipose tissue lipid profiles by high performance liquid chromatography,” M.S. - Master of Science, Middle East Technical University, 2013.