Physiological and biochemical screening of different Turkish lentil cultivars under salt stress conditions

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2012

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Köse, Fatma Selin

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Salinity is the 2nd major limiting abiotic factor on plant growth. As a result of this, soil salinity greatly reduce the yield of the crop production by dual action on plants which are ionic toxicity and water deficit. Therefore, improvement of stress tolerance is greatly concerned. This study was performed to screen and select a salt-resistant and a salt-sensitive cultivar among 6 Turkish lentil cultivars (Lens culinaris M.) which are Çağıl, Çiftçi, Kafkas, Malazgirt, Seyran and Özbek according to the physiological and biochemical properties. 12 days old lentil seedlings which were exposed to salt stress (100 mM NaCl and 150 mM NaCl) for 5 days as well as control groups analyzed physiologically by root-shoot fresh weights, and lengths; and biochemically by ion leakage, MDA, H₂O₂ and proline content determination. Seyran and Malazgirt were selected as salt-resistant and salt-sensitive, respectively. Selected two cultivars were also compared in terms of antioxidative defense system (SOD, CAT, APX and GR activity determination). Bioanalyzer and SDS-PAGE were performed to contrast total protein profile of salt-resistant and salt-sensitive cultivars.

Subject Keywords

Lens (Plants) ., Lentils, Soils, Salts in.

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http://etd.lib.metu.edu.tr/upload/12615009/index.pdf
https://hdl.handle.net/11511/21924

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Graduate School of Natural and Applied Sciences, Thesis

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Citation Formats

F. S. Köse, “Physiological and biochemical screening of different Turkish lentil cultivars under salt stress conditions,” M.S. - Master of Science, Middle East Technical University, 2012.