Physiological and biochemical screening of different Turkish lentil cultivars under salt stress conditions

Download
2012
Köse, Fatma Selin
Salinity is the 2nd major limiting abiotic factor on plant growth. As a result of this, soil salinity greatly reduce the yield of the crop production by dual action on plants which are ionic toxicity and water deficit. Therefore, improvement of stress tolerance is greatly concerned. This study was performed to screen and select a salt-resistant and a salt-sensitive cultivar among 6 Turkish lentil cultivars (Lens culinaris M.) which are Çağıl, Çiftçi, Kafkas, Malazgirt, Seyran and Özbek according to the physiological and biochemical properties. 12 days old lentil seedlings which were exposed to salt stress (100 mM NaCl and 150 mM NaCl) for 5 days as well as control groups analyzed physiologically by root-shoot fresh weights, and lengths; and biochemically by ion leakage, MDA, H₂O₂ and proline content determination. Seyran and Malazgirt were selected as salt-resistant and salt-sensitive, respectively. Selected two cultivars were also compared in terms of antioxidative defense system (SOD, CAT, APX and GR activity determination). Bioanalyzer and SDS-PAGE were performed to contrast total protein profile of salt-resistant and salt-sensitive cultivars.

Suggestions

Physiological and biochemical screeing of different Turkish Lentil (Lens culinaris M.) cultivars under drought stress condition
Gökçay, Derya; Yücel, Ayşe Meral; Baloğlu, Mehmet Cengiz; Department of Biotechnology (2012)
Legumes being the most important crops worldwide are limited in terms of adaptability and productivity mainly by the abiotic stresses. In this study, the aim was to understand tolerance mechanisms of lentil cultivars under drought stress by physiological and biochemical analyses. This study was carried out with six Turkish Lentil cultivars (Seyran, Kafkas, Malazgirt, Çağıl, Çiftçi, Özbek) subjected to drought stresses (10% and 15% PEG) and their physiological and biochemical properties were examined to sele...
Transformation of nicotiana tabacum plants with na+/h+ antiporter (atnhx1) gene isolated from arabidopsis thaliana for evaluation of salt tolerance
Aysin, Ferhunde; Erson Bensan, Ayşe Elif; Department of Biology (2007)
Large, membrane-bound vacuoles of plant cells are suitable organelles for the compartmentation of ions. These vacuoles contain Na+/H+ antiporters for movement of Na+ within the organelle in exchange for H+. They provide an efficient mechanism to prevent the occurance of detrimental outcomes of Na+ accumulation in the cytosol. Identification of AtNHX1 gene that confers resistance to salinity by expressing a Na+/H+ antiport pump facilitates the understanding of the salt stress tolerance mechanisms of plants. ...
Evaluation of salt tolerance in sto transformed Arabidopsis thaliana and Nicotiana tabacum plants
Selçuk, Feyza; Yücel, Ayşe Meral; Department of Biology (2004)
Salinity is one of the limiting factors of crop development. Together with causing water loss from plant tissues, salinity also leads to ion toxicity. Under salt stress, increase in Ca+2 concentration in cytosol can decrease the deleterious effects of stress. The binding of Ca+2 to calmodulin initiates a signaling cascade involving the activation of certain transcription factors like STO and STZ. This signal transduction pathway regulates transport of proteins that control net Na+ influx across the plasma m...
Comparative study of soil water characteristic curve prediction methods
Ahmadiadli, Mohammad; Huvaj Sarıhan, Nejan; Toker, Nabi Kartal; Kürkçü, Mefküre Gamze (2012-06-30)
The soil-water characteristic curve (SWCC), which defines the relation between soil suction and water content, plays a key role in geotechnical studies of the unsaturated soils. Some researchers such as Brooks & Corey (1968), Van Genuchten (1982) and Fredlund& Xing (1994) have proposed curve fitting equations for SWCC. Fitting equations to the laboratory test data obtained from direct measurement of water content versus changes in matric suction is the most reliable method, but it is expensive and time cons...
Exponential Equation for Predicting Shear Strength Envelope of Unsaturated Soils
Ahmadi Naghadeh, Reza; Toker, Nabi Kartal (2019-07-01)
An exponential equation is introduced to predict the nonlinear variation of shear strength with matric suction for unsaturated soils. The proposed equation involves three constant parameters, two of which are effective shear strength parameters (i.e., phi ' and c '). The third parameter is the maximum capillary cohesion, c '' max, which is the maximum possible increase in shear strength due to matric suction. A procedure for the determination of c '' max from the soil-water characteristic curve (SWCC) is de...
Citation Formats
F. S. Köse, “Physiological and biochemical screening of different Turkish lentil cultivars under salt stress conditions,” M.S. - Master of Science, Middle East Technical University, 2012.