Transformation of tobacco with a NAC type transcription factor, tanac69-1 and characterization of transgenic plants via molecular and physiological techniques

Download
2015
Eroğlu, Ayten
Environmental stresses such as drought and salinity greatly affect plant production leading to reduction in yield and quality. Stress tolerant plants can be rapidly produced by transferring stress related genes or transcription factors to crop plants. In this study, TaNAC69-1 gene, a NAC type transcription factor, was isolated from Triticum aestivum L. cv. Yüreğir-89 and cloned into a Gateway compatible overexpression vector, pEarleyGate 100. Transformation of Nicotiana tabacum L. cv. Samsun leaves was carried out via Agrobacterium-mediated transformation method. A total of fourty five T0 putative transgenic tobacco lines were obtained at the end of independent transformation experiments. Stable expression and integration of TaNAC69-1 gene into the tobacco genome were evaluated by molecular, biochemical and physiological analysis. Traditional PCR was made by using gene specific and bar gene specific primers to screen transgenes. Stable integration of TaNAC69-1 gene into the genome was also confirmed by Southern blot analysis. vi Expression of TaNAC69-1 gene was examined by qRT-PCR with different time periods under salt and drought stress conditions using actin as a reference gene. It was found that drought and salt treatments led to significant up-regulation of TaNAC69-1 gene expression. Physiological assays such as germination, root and shoot lengths, fresh and dry weights were achieved to compare stress tolerance of wild type and transgenic tobacco lines under salt and osmotic stress conditions. Germination and survival rates of transgenic tobacco seeds were significantly higher than wild type seeds when subjected to NaCl and LiCl treatment. Root and shoot lengths, fresh and dry weights of transgenic plants significantly increased when compared to wild type plants with application of NaCl and LiCl. On the other hand there was no difference between growth parameters of wild type and transgenic lines with mannitol treatment. According to molecular and physiological analysis, overexpression of TaNAC69-1 gene increased salt and ionic stress tolerance of transgenic tobacco plants. Further experiments should be done with cold and heat stress treatments to better elucidate the function of TaNAC69-1gene under abiotic stress conditions.

Suggestions

Quantifying the streamflow response to groundwater abstractions for irrigation or drinking water at catchment scale using SWAT and SWAT-MODFLOW
Liu, Wei; Park, Seonggyu; Bailey, Ryan T.; Molina-Navarro, Eugenio; Andersen, Hans Estrup; Thodsen, Hans; Nielsen, Anders; Jeppesen, Erik; Jensen, Jacob Skodt; Jensen, Jacob Birk; Trolle, Dennis (Springer Science and Business Media LLC, 2020-09-01)
Background Groundwater abstraction can cause a decline in the water table, and thereby affects surface streamflow connected to the aquifer, which may impair the sustainability of both the water resource itself and the ecosystem that it supports. To quantify the streamflow response to groundwater abstractions for either irrigation or drinking water at catchment scale and compared the performance of the widely used semi-distributed hydrological model SWAT and an recently integrated surface-subsurface model SW...
Enhanced salt tolerance of transgenic tobacco expressing a wheat salt tolerance gene
KAVAS, MUSA; BALOĞLU, MEHMET CENGİZ; Yücel, Ayşe Meral; Öktem, Hüseyin Avni (2016-01-01)
Soil salinity is one of the most important limiting factors of agricultural productivity in the world. The Triticum aestivum salt tolerance-related gene (TaSTRG) possesses a functional response to salt and drought stress conditions. A variety of stress factors, such as salt, drought, abscisic acid, and cold, may induce the expression of TaSTRG in wheat. In this study, the TaSTRG gene was transferred to tobacco via Agrobacterium-mediated transformation. Overexpression of TaSTRG in transgenic tobacco plants i...
Effects of operational parameters on the settling properties of activated sludge
Sürücü, Gülerman; Soyupak, Salçuk (Informa UK Limited, 1989-5)
Settling properties of activated sludge typically establish the performance and the cost of biological wastewater treatment plants, and they are considerably affected by the solids concentration of the sludge and the mean cell residence time of the system. In this work, effects of mean cell residence time of the system and solids concentration of sludge on the settling properties of activated sludge were studied. By statistical analysis of the laboratory data, mathematical expressions were suggested to e...
Particle bombardment transformation of some Turkish wheat cultivars with TaNAC69-1 and TtNAMB2 genes
Battal, A.; BALOĞLU, MEHMET CENGİZ; Kavas, M.; Yücel, Mustafa; Öktem, Hüseyin Avni (2012-09-23)
Wheat is one of the most important crop plants in the world.The production of wheat is seriously affected by biotic (pests,bacterial and fungal diseases) and abiotic (drought, salinity andfreezing) stresses. NAC family proteins (NAM/ATAF1-2/CUC2) areplant specific transcription factors and related with development,defense and abiotic stress responses.TaNAC69-1andTtNAMB2genes are the transcription factors from this protein family. Theaim of this study was to transform of some Turkish ...
Tracking transformation processes of organic micropollutants in aquatic environments using multi-element isotope fractionation analysis
Hofstetter, Thomas B.; Bolotin, Jakov; Skarpeli-Liati, Marita; Wijker, Reto; Kurt, Zöhre; Nishino, Shirley F.; Spain, Jim C. (Elsevier BV, 2011-06-01)
The quantitative description of enzymatic or abiotic transformations of man-made organic micropollutants in rivers, lakes, and groundwaters is one of the major challenges associated with the risk assessment of water resource contamination. Compound-specific isotope analysis enables one to identify (bio)degradation pathways based on changes in the contaminants' stable isotope ratios even if multiple reactive and non-reactive processes cause concentrations to decrease. Here, we investigated how the magnitude ...
Citation Formats
A. Eroğlu, “Transformation of tobacco with a NAC type transcription factor, tanac69-1 and characterization of transgenic plants via molecular and physiological techniques,” Ph.D. - Doctoral Program, Middle East Technical University, 2015.