Proteome-wide analysis of the role of expression of bacilysin operon on idiophase physiology of B. Subtilis

Download

index.pdf

Date

2013

Author

Demir, Mustafa

Metadata

Show full item record

Item Usage Stats

258
views

116
downloads

The members of the genus Bacillus produce a wide variety of secondary metabolites with antimetabolic and pharmacological activities. These metabolites are mostly small peptides and have unusual components and chemical bonds. These metabolites are synthesized nonribosomally by multifunctional enzyme complexes called peptide synthetases. One of those small peptides, bacilysin, is a dipeptide antibiotic composed of L-alanine and L-anticapsin which is produced and excreted by certain strains of Bacillus subtilis. Proteins that are responsible to synthesize bacilysin are encoded by bac operon. It has been shown that the biosynthesis of bacilysin is under the control of quorum sensing global regulatory pathway through the action of ComQ/ComX, PhrC (CSF), ComP/ComA in a Spo0K (Opp)-dependent manner. The objective of the study is to identify the functional roles of bacilysin biosynthesis in the regulatory cascade and idiophase cell physiology operating in B. subtilis by using gel-based and gel-free proteomics techniques. For this, we employed comparative proteome-wide analysis of the bacilysin producer B. subtilis PY79 and its bacilysin nonproducer derivative bacA::lacz::erm OGU1 strain which was recently constructed by our group. Identification via GeLC analysis of 76 differentially expressed proteins from total soluble proteome of wild-type PY79 and bacilysin minus OGU1 strain indicated the direct or indirect multiple effects of bacilysin on metabolic pathways, global regulatory systems and sporulation.

Subject Keywords

Bacillus (Bacteria), Bacillus subtilis, Proteomics., Quorum sensing (Microbiology).

URI

http://etd.lib.metu.edu.tr/upload/12615570/index.pdf
https://hdl.handle.net/11511/22443

Collections

Graduate School of Natural and Applied Sciences, Thesis

Suggestions

OpenMETU
Core

Proteome-wide analysis of the functional roles of bacilysin biosynthesis in Bacillus subtilis Özcengiz, Gülay; Demir, Mustafa; Karatas, Ayten Yazgan (2014-07-01) The members of the genus Bacillus produce a wide variety of secondary metabolites with antimetabolic and pharmacological activities. Most of these metabolites are small peptides that have unusual components and chemical bonds and synthesized nonribosomally by multifunctional enzyme complexes called peptide synthetases. Bacilysin, being produced and excreted by certain strains of Bacillus subtilis, is one of the simplest peptide antibiotics known. It is a dipeptide with an N-terminal Lalanine and an unusual ...
Proteome-wide analysis of the functional roles of Bacilysin biosynthesis in Bacillus subtilis Taşkın Aras, Aslı; Özcengiz, Gülay; Department of Biology (2010) The members of the genus Bacillus produce a wide variety of secondary metabolites with antimetabolic and pharmacological activities. Most of these metabolites are small peptides that have unusual components and chemical bonds and synthesized nonribosomally by multifunctional enzyme complexes called peptide synthetases. Bacilysin, being produced and excreted by certain strains of Bacillus subtilis, is one of the simplest peptide antibiotics known. It is a dipeptide with an N-terminal L-alanine and an unusual...
Biological network modelling based on differentially expressed proteins in a bacilysin-deficient strain of bacillus subtilis Kutnu, Meltem; Özcengiz, Gülay; Department of Molecular Biology and Genetics (2019) Bacillus subtilis is a non-pathogenic, Gram-positive organism which is known for producing a broad range of secondary metabolites with pharmacological and antimicrobial activities. The dipeptide bacilysin is one of the many antibiotics synthesized by certain strains of B. subtilis, and it is composed of L-alanine and the non-proteinogenic amino acid L-anticapsin. Earlier silencing studies by our group have suggested that bacilysin acts as a pleiotropic molecule on its host. Therefore, the absence or lack of...
Dynamic analysis of secretome alterations in a bacilysin-knock out mutant of bacillus subtilis Tekin İşlerel, Elif; Özcengiz, Gülay; Department of Biology (2017) The members of the genus Bacillus produce a wide variety of secondary metabolites with antimetabolic and pharmacological activities. Bacilysin, being produced and excreted by certain strains of B. subtilis, is a dipeptide antibiotic composed of L-alanine and L-anticapsin which is synthesized enzymatically. As shown earlier by our research group, the expression of bacilysin biosynthetic operon (bacA) is relatively constant during the exponential growth, but increases during the transition between exponential...
Protease secretion capacity and perforce analysis of recombinant Bacillus species Çalık, Pınar; Ozdamar, TH (2000-10-08) Recombinant Bacillus species carrying subC gene encoding serine alkaline protease (SAP) enzyme were developed in order to increase the yield and selectivity in the bioprocess for SAP production. subC gene was amplified from the chromosomal DNA of the wild-type Bacillus licheniformis by using PCR technology; thereafter, subC gene was first cloned into the pRS316 E. coli yeast shuttle plasmid, then sub-cloned into the pHV1431 E. coli-Bacillus shuttle vector, and transferred to the host Bacillus species, i.e. ...

Citation Formats

M. Demir, “Proteome-wide analysis of the role of expression of bacilysin operon on idiophase physiology of B. Subtilis,” M.S. - Master of Science, Middle East Technical University, 2013.