Sequence specific inhibition of human chemokine gene, cxcl7, by small interfering RNAS in lewis lung carcinoma cells

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2013

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Kızışar, Dilay

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In this study, we aimed to design small interfering RNAs to silence the expression of a gene that is a member of chemokines playing crucial roles on cancer. Small interfering RNAs (siRNAs) are generated by ribonuclease III cleavage of 20-25nt in length dsRNAs which mediate RNAi-induced specific mRNA degradation and they are known to be an important part of the gene regulation mechanism in all Eukaryotes. Recently, siRNAs are being utilized as promising treatment approaches. In this study, we targeted a gene involved in lung cancer CXCL7 via designing potent siRNA sequences and carried out optimization for efficient transfection. The level of silencing in mouse cell culture was determined using a Flow Cytometry.

Subject Keywords

Chemokines, RNA, Gene silencing., Cytometry., Lungs

URI

http://etd.lib.metu.edu.tr/upload/12616519/index.pdf
https://hdl.handle.net/11511/22960

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Graduate School of Natural and Applied Sciences, Thesis

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Citation Formats

D. Kızışar, “Sequence specific inhibition of human chemokine gene, cxcl7, by small interfering RNAS in lewis lung carcinoma cells,” M.S. - Master of Science, Middle East Technical University, 2013.