Monitoring of cobalt adaptation of environmental bacterial isolates using ATR-FTIR spectroscopy

Download
2014
Kardaş, Mehmet
This thesis investigates the effects of cobalt acclimation in three distinct bacteria. Heavy metals at high concentration can be toxic for bacteria. However, upon prolonged exposure bacteria can acclimate to grow in the presence of heavy metals. As a result of the acclimation, bacteria alter their metabolism and molecular structures. Aim of this study was to examine these alterations in cobalt acclimated bacteria via attenuated total reflection Fourier transform infrared (ATR-FTIR) spectroscopy on viable samples. Bacillus sp., and Pseudomonas sp., isolated from a temperate shallow lake, and a well-established strain of E. coli were investigated. Results hinted drastic changes in cell membrane and wall dynamics of Bacillus sp. and E. coli, including a decrease in peptidoglycan content of Bacillus sp. and the increased lipid ordering of the membrane in both bacteria. Furthermore, a decrease in RNA and protein concentrations of Bacillus sp. is measured. All three bacteria studied showed a decrease in conformational freedom of proteins in their cobalt acclimated status. Interestingly, both Bacillus sp. and E. coli showed slight but significant alterations in their DNA conformations which might imply a methylation mediated memory formation leading to epigenetic modulation for cobalt adaptation.

Suggestions

Screening of Protective Effect of Amifostine on Radiation-Induced Structural and Functional Variations in Rat Liver Microsomal Membranes by FT-IR Spectroscopy
Cakmak, Gulgun; Zorlu, Faruk; Severcan, Mete; Severcan, Feride (2011-04-01)
In this study, the protective effect of amifostine, which is the only FDA-approved radioprotective agent, was investigated against the deleterious effects of ionizing radiation on rat liver microsomal membranes at molecular level. Sprague-Dawley rats, which were either administered amifostine or not, were whole-body irradiated with a single dose of 800 cGy and decapitated after 24 h. The microsomal membranes isolated from the livers of these rats were investigated using FT-IR spectroscopy. The results revea...
The characterization of bacteria with Fourier Transform Infrared (FTIR) spectroscopy
Garip, Şebnem; Severcan, Feride; Department of Biology (2005)
New and rapid techniques for the characterization and identification of bacteria would have an important role in clinical microbiology and in food analysis because of an increasing prevalence of infectious diseases and In this work we carried out two approaches. In the first study the characterization and differentiation of mesophilic and thermophilic bacteria were investigated by using Fourier Transform Infrared (FTIR) Spectroscopic technique. In the second study, we investigated the characterization and i...
The structural effects of Vitamin A deficiency on biological macromolecules due to ethanol consumption and withdrawal: An FTIR study with chemometrics
ELİBOL, BİRSEN; Severcan, Mete; Jakubowska-Dogru, Ewa; DURSUN, İLKNUR; Severcan, Feride (2022-04-01)
The structural effects of vitamin A-deficiency were examined on the molecular profiles of biomolecules of male rat hippocampus during prolonged ethanol intake/withdrawal using FT-IR spectroscopy coupled with chemometrics. Liquid ethanol diet with/without vitamin A was maintained to adult rats for 3-months. The rats were decapitated at different ethanol withdrawal times and FT-IR spectra were obtained. Ethanol consumption/withdrawal produced significant changes in proteins' conformations, while having insign...
Identification of serotype specific dna marker for salmonella typhimurium by rapd-pcr method
Aksoy, Ceren; Gültekin, Güzin Candan; Department of Biotechnology (2004)
This study was performed for the identification of specific DNA marker using RAPD-PCR (Random Amplified Polymorphic DNA) method for serotype Salmonella Typhimurium which is one of the most prevalent serotype causing food poisining all over the world. The Primer 3 (RAPD 9.1), 5̕-CGT GCA CGC-3ء, was used in RAPD-PCR with 35 different Salmonella isolates. 12 of them were serotype Salmonella Typhimurium and 23 of them were belonging to other six different serotypes. Accordingly, two different 300 bp and 700 bp ...
Aspects of silver tolerance in bacteria: infrared spectral changes and epigenetic clues
Gurbanov, Rafig; Ozek, Nihal S.; Tunçer, Sinem; Severcan, Feride; Gözen, Ayşe Gül (2018-05-01)
In this study, the molecular profile changes leading to the adaptation of bacteria to survive and grow at inhibitory silver concentration were explored. The profile obtained through infrared (IR)-based measurements indicated extensive changes in all biomolecular components, which were supported by chemometric techniques. The changes in biomolecular profile were prominent, including nucleic acids. The changes in nucleic acid region (1350-950 cm(-1)) were encountered as a clue for conformational change in DNA...
Citation Formats
M. Kardaş, “Monitoring of cobalt adaptation of environmental bacterial isolates using ATR-FTIR spectroscopy,” M.S. - Master of Science, Middle East Technical University, 2014.