The structural effects of Vitamin A deficiency on biological macromolecules due to ethanol consumption and withdrawal: An FTIR study with chemometrics

2022-04-01
ELİBOL, BİRSEN
Severcan, Mete
Jakubowska-Dogru, Ewa
DURSUN, İLKNUR
Severcan, Feride
Show full item record
Creative Commons License
This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.
261
views
0
downloads
The structural effects of vitamin A-deficiency were examined on the molecular profiles of biomolecules of male rat hippocampus during prolonged ethanol intake/withdrawal using FT-IR spectroscopy coupled with chemometrics. Liquid ethanol diet with/without vitamin A was maintained to adult rats for 3-months. The rats were decapitated at different ethanol withdrawal times and FT-IR spectra were obtained. Ethanol consumption/withdrawal produced significant changes in proteins' conformations, while having insignificant structural effects on lipids. In vitamin A deficiency, ethanol produced structural changes in lipids by lipid ordering especially in the early-ethanol withdrawal. Furthermore, an increase in lipid and protein content, saturated/unsaturated lipid ratio, a decrease in nucleic acids content and decrease in membrane fluidity were observed. These changes were less severe in the presence of Vitamin A. This study is clinically important for individuals with vitamin A deficiency because they have to be more cautious when consuming alcohol to protect themselves from cognitive dysfunctions.
ethanol, FT-IR spectroscopy, male rat hippocampus, vitamin a deficiency, TRANSFORM INFRARED-SPECTROSCOPY, PHOSPHOLIPID MODEL MEMBRANES, LIPID-PEROXIDATION, RAT-LIVER, OXIDATIVE STRESS, FUNCTIONAL ALTERATIONS, IR SPECTROSCOPY, IN-VITRO, BRAIN, ALCOHOL
https://hdl.handle.net/11511/97183
JOURNAL OF BIOPHOTONICS
Department of Biology, Article

Suggestions

The effect of arginine-428 mutation on modulation of activity of human liver flavin monooxygenase 3 (FMO3) by imipramine and chlorpromazine
Adalı, Orhan; Philpot, RM (1999-07-01)
This study was carried out to investigate the molecular basis for modulation of recombinant FMO3-catalyzed activity by the tricyclicantidepressants, imipramine and chlorpromazine. A mutant of human liver FMO3 (T428R) was formed by site-directed mutagenesis and characterized along with the native enzyme in order to elucidate a possible structure-function relationship. Functional properties of native and T428R human FMO3s were studied with methimazole as substrate. Both enzymes catalyzed the S-oxidation of me...
The effect of diabetes on rat skeletal muscle tissues at molecular level
Bozkurt, Özlem; Severcan, Feride; Department of Biology (2006)
In the present study Fourier Transform Infrared Spectroscopy was used to examine the effects of streptozotocin-induced diabetes mellitus on the structural components of slow- and fast-twitch rat skeletal muscles, at molecular level. Diabetes mellitus is a chronic disorder of carbohydrate, fat and protein metabolism, which is characterized by hyperglycemia caused by a defective or deficient insulin secretory response. The effect of diabetes is seen on a variety of tissues leading to important secondary compl...
The characterization of bacteria with Fourier Transform Infrared (FTIR) spectroscopy
Garip, Şebnem; Severcan, Feride; Department of Biology (2005)
New and rapid techniques for the characterization and identification of bacteria would have an important role in clinical microbiology and in food analysis because of an increasing prevalence of infectious diseases and In this work we carried out two approaches. In the first study the characterization and differentiation of mesophilic and thermophilic bacteria were investigated by using Fourier Transform Infrared (FTIR) Spectroscopic technique. In the second study, we investigated the characterization and i...
The effects of early postnatal ethanol intoxication on retina ganglion cell morphology and the development of retino-geniculate projections in mice
Dursun, İlknur; Jakubowska Doğru, Ewa; Department of Biology (2011)
Experimental and clinical data have documented the adverse effects of perinatal ethanol intoxication on peripheral organs and the central nervous system. There is little known, however, about potential damaging effects of perinatal ethanol on the developing visual system. The purpose of this study was to examine the effects of neonatal ethanol intoxication on RGC morphology, estimate the total number of neurons in RGC layer and dorsolateral geniculate nucleus (dLGN), and on the eye-specific fiber segregatio...
The inhibitory effects of lindane in batch and upflow anaerobic sludge blanket reactors
Bayramoğlu, Tuba Hande; Demirer, Göksel Niyazi (Elsevier BV, 2003-01-01)
In this study, the inhibitory effects of lindane (LIN) on originally unacclimated mixed anaerobic cultures were investigated by anaerobic toxicity assay (ATA) experiments. ATA experiments revealed that 10 mg/l LIN exerted inhibitory effects on anaerobic cultures, which was recoverable. Continuous reactor experiments conducted to determine the inhibitory effects of LIN and the maximum LIN loading rate achievable in two-stage upflow anaerobic sludge blanket (UASB) reactors indicated that anaerobic granular cu...
Citation Formats
B. ELİBOL, M. Severcan, E. Jakubowska-Dogru, İ. DURSUN, and F. Severcan, “The structural effects of Vitamin A deficiency on biological macromolecules due to ethanol consumption and withdrawal: An FTIR study with chemometrics,” JOURNAL OF BIOPHOTONICS, pp. 0–0, 2022, Accessed: 00, 2022. [Online]. Available: https://hdl.handle.net/11511/97183.
METU IIS - Integrated Information Service open@metu.edu.tr