Genetic control of cellulose, lignin and glucose contents in european black poplar (populus nigra l.) populations from Turkey

Taşkıran, Bircan
Populus nigra L. is considered as one of the most economically significant forest tree species with respect to production of wood, biomass, timber, pulp, paper and other wood-based products, besides its ecological and evolutionary importance. Because of the increased wood needs of the world and demands of renewable energy sources, fast-growing poplar has gained importance. While wood quality, pulp mechanical strength, and biomass are directly associated with high cellulose content, lignin emerges as an undesirable polymer for both pulp and biofuel manufacturing industries. To estimate the contents of the main cell wall polymers, cellulose, lignin, and total D-glucose content of wood cell, and also their proportion to each other, we established a large collection of biological samples from one year old branches of poplar trees (285 clones x 3 replicates x 2 ramets) which were grown for three years in an outdoor nursery (Behiçbey Nursery) in Ankara. These clones were originally collected from all over Turkey. Additionally, five commercially registered clones and six foreign clones were included in the study for comparison. The mean values for cellulose, lignin and glucose content were 21.8 ± 16.29 µg/ml, 23 ± 4.64 µg/ml, and 35 ± 9.71 µg/ml, respectively. However, particular clones were detected with extraordinary high values for these traits. The components of the total variance due to among clones within regions were 15.11 %, 3.47 %, and 12.94 % for cellulose, lignin and glucose, respectively. Positive correlations were observed between cellulose and height (r = 0.22; p < 0.01), cellulose and diameter (r = 0.23; p < 0.01) and also between lignin and glucose (r = 0.31, p < 0.01). On the other hand, negative correlation was detected between lignin and diameter (r = -0.121; p < 0.05) as expected. In the study, all clones were also evaluated with respect to high quality and yield to realize valuable clones with high cellulose to low lignin content. The superior clones were 62172, N91075, 62160, N03377, and 62191, whereas the inferior clones were 641410, N91085, 8511, N03372, and N92169. Also the study by choosing the superior and eliminating the inferior clones pave the way for future investments and applicable new poplar breeding programs.


Genetic diversity of european black poplar (populus nigra) populations from Turkey assessed by microsatellite DNA markers
Çiftçi, Asiye; Kaya, Zeki; Department of Biology (2013)
The genus Populus is known as a group of forest trees for commercial exploitation. The European black poplar (Populus nigra L.), belonging to the genus Populus, is a keystone species of floodplain forests in Europe. Populus nigra has also been used as a model tree in the study of genetic resource conservation of wild relatives of cultivated plants. Turkey has over 68.000 ha poplar plantations with P. nigra and 77. 000 ha consisting of various hybrids of it. Due to overexplotation, loss of natural distributi...
Genetic control of 4 coumarate coenzyme A ligase (4CL9 enzymes involved in lignin biosynthesis of European black poplar (Populus nigra L.)
Taşkıran, Bircan; Güray, Nülüfer Tülün; Kaya, Zeki (null; 2018-01-13)
European black poplar (Populus nigra L.) is considered as one of the most economically significant forest trees with respect to production of wood, pulp, bioenergy and other wood-based products. The content and quality of wood are greatly affected by plant cell wall compositions (cellulose, hemicellulose, and lignin). Although lignin serves as a mechanical barrier as well as protection against pests and pathogens, it emerges as an undesirable polymer for both pulp and bioenergy manufacturing industries beca...
Genetic differentiation of European black poplar (Populus Nigra L.) clones and populations with respect to some enzymes involved in biosynthesis of cellulose and lignin
Taşkıran, Bircan; Kaya, Zeki; Department of Biology (2020)
The European black poplar, (Populus nigra L.) is ecologically and economically important fast-growing trees. Its wide distribution range in northern hemisphere, easy vegetative propagation, rapid growth rate and hybridization ability, and also its biomass source potential make European black poplar a perfect candidate for lignocellulosic biomass production. In this study, specific activities of two important cellulose related (SuSy and UGPase) and three lignin pathway related (PAL, 4CL, and CAD) enzymes, we...
Genetic diversity pattern in Populus Nigra populations of two major river systems in Turkey
Çiftçi, Asiy; Kaya, Zeki; Department of Biology (2019)
European black poplar (Populus nigra L.) is an important tree species in terms of social, economic and ecological interest in Turkey. Although, poplar plantations in large areas meet the needs of the economy, the natural genetic resources of the species have been highly degraded due to anthropogenic effects and natural biological events. To assess genetic diversity structure of natural populations, 124 naturally distributed European black poplar trees from two major rivers (Kızılırmak and Göksu) in Turkey w...
Differential expression of cold resistant related genes in populus nigra l. clones
Zeybek, Evrim; Kaya, Zeki; Department of Biology (2014)
Poplar has been adopted as a model perennial woody species for forest tree genetics to understand molecular processes of growth, development and responses to environmental stresses. In this study, seasonal changes in antioxidant enzymes activities including glutathione reductase (GR), ascorbate peroxidase (APX), dehydroascorbate reductase (DHAR) and catalase (CAT) were examined in black poplar (Populus nigra L.) clones. It was found that GR, APX and DHAR activities increased with low air temperatures in win...
Citation Formats
B. Taşkıran, “Genetic control of cellulose, lignin and glucose contents in european black poplar (populus nigra l.) populations from Turkey,” M.S. - Master of Science, Middle East Technical University, 2014.