Genetic diversity of european black poplar (populus nigra) populations from Turkey assessed by microsatellite DNA markers

Çiftçi, Asiye
The genus Populus is known as a group of forest trees for commercial exploitation. The European black poplar (Populus nigra L.), belonging to the genus Populus, is a keystone species of floodplain forests in Europe. Populus nigra has also been used as a model tree in the study of genetic resource conservation of wild relatives of cultivated plants. Turkey has over 68.000 ha poplar plantations with P. nigra and 77. 000 ha consisting of various hybrids of it. Due to overexplotation, loss of natural distribution area and introgression, P. nigra is one of the most threatened tree species in Turkey and Europe. The important and major genetic resources of European black poplar for Turkey consist of 297 clones and located in Behiçbey clone bank in Ankara with perspective of ex situ conservation programme for future plantations. To characterize genetic diversity in European black poplar populations and to provide genetic identity information for these, 297 P. nigra clones were analysed by using 12 microsatellite DNA markers. Also, 32 trees from newly discovered two natural populations were included into the analyses to compare them with the clone bank populations with respect to genetic diversity. The number of alleles ranged from 3.36 to 8.08 for 12 populations. The observed heterozygosity was found to be between 0.62 and 0.69. About 8 % of total genetic variation is between clone bank and natural populations. Great portion of total genetic variation (92%) is within the populations present in the clone bank. When all European black poplar populations (clone bank and natural populations) were evaluated with respect to genetic relatedness, clone bank and natural populations formed two separate group in the dendogram. Foreign and open pollinated populations indicated a close association to natural populations. In the current study, with the help of microsatellite DNA markers, identity problems of clones were solved. Furthermore, magnitute and pattern of genetic diversity in populations from clone bank and natural distribution areas were assessed and clonally duplicated trees were successfully determined. All populations have sufficient genetic diversity to carry out further breeding programmes for European black poplar. There have been found many vi clonally duplicated trees in the clone bank. For further breeding studies, these clones have to be replaced with new selected individuals from genetic diversity rich areas.


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Taşkıran, Bircan; Kaya, Zeki; Severcan, Feride; Department of Biology (2014)
Populus nigra L. is considered as one of the most economically significant forest tree species with respect to production of wood, biomass, timber, pulp, paper and other wood-based products, besides its ecological and evolutionary importance. Because of the increased wood needs of the world and demands of renewable energy sources, fast-growing poplar has gained importance. While wood quality, pulp mechanical strength, and biomass are directly associated with high cellulose content, lignin emerges as an unde...
Genetic diversity pattern in Populus Nigra populations of two major river systems in Turkey
Çiftçi, Asiy; Kaya, Zeki; Department of Biology (2019)
European black poplar (Populus nigra L.) is an important tree species in terms of social, economic and ecological interest in Turkey. Although, poplar plantations in large areas meet the needs of the economy, the natural genetic resources of the species have been highly degraded due to anthropogenic effects and natural biological events. To assess genetic diversity structure of natural populations, 124 naturally distributed European black poplar trees from two major rivers (Kızılırmak and Göksu) in Turkey w...
Genetic diversity structure of populus nigra populations in a major rivers system. what is the role of human impact?
Çiftci, Asiye; Kaya, Zeki (2017-09-22)
Populus nigra is one of the most economically and ecologically important forest trees in Turkey, well known for its rapid growth, good ability to vegetative propagation and the extreme uses of its wood. Due to overexploitation, loss of natural distribution area and extreme hybridization and introgression, Populus nigra is one of the most threatened tree species in Turkey and Europe. Using seventeen nuclear SSR loci, the genetic structure of European black poplar populations along the largest river of Turkey...
Genetic differentiation of European black poplar (Populus Nigra L.) clones and populations with respect to some enzymes involved in biosynthesis of cellulose and lignin
Taşkıran, Bircan; Kaya, Zeki; Department of Biology (2020)
The European black poplar, (Populus nigra L.) is ecologically and economically important fast-growing trees. Its wide distribution range in northern hemisphere, easy vegetative propagation, rapid growth rate and hybridization ability, and also its biomass source potential make European black poplar a perfect candidate for lignocellulosic biomass production. In this study, specific activities of two important cellulose related (SuSy and UGPase) and three lignin pathway related (PAL, 4CL, and CAD) enzymes, we...
Genetic differentiation between clone collections and natural populations of European black poplar (Populus nigra L.) in turkey
Çiftçi, Asiye; Kucukosmanoglu, Filiz; Karahan, Alptekin; Kaya, Zeki (2017-06-01)
The European black poplar (Populus nigra L.) is an ecologically and economically important tree species for Turkey. The important and major genetic resources of species for future breeding and ex situ conservation purposes have been archived in a clone bank in Ankara by selecting clones from natural populations and old plantations throughout Turkey. There is no study to date assessing genetic composition these materials. Two-hundred-thirty-three P. nigra clones from six geographic region of Turkey (clone co...
Citation Formats
A. Çiftçi, “Genetic diversity of european black poplar (populus nigra) populations from Turkey assessed by microsatellite DNA markers,” M.S. - Master of Science, Middle East Technical University, 2013.