Bio-fuel production from microalgae

Download

index.pdf

Date

2015

Author

Onay, Melih

Metadata

Show full item record

Item Usage Stats

240
views

162
downloads

Bio-fuel is a renewable fuel and it includes different biofuel energy sources such as methane, bioethanol and biodiesel. Generally, biodiesel is produced from agricultural waste, vegetable oils such as soybean and palm oil. Third generation biofuels called as microalgae have been appeared nowadays. In the current study, biodiesel production from thermo-resistant green microalgae was focused on. For this aim, microalgae were sampled from a few hotspring points in Haymana and isolated. Identification and characterization of isolated microalgae were performed morphologically via scanning electron microscopy and molecularly via ITS2 region. They were named as Scenedesmus sp.ME02, Hindakia tetrachotoma ME03 and Micractinium sp. ME05. Also, three different solvent extraction methods and five cell disruption techniques (Homogenization Assisted, Microwave Assisted, Ultrasonnication, Glass Bead and Lyophilization Assisted) were applied for effective lipid recovery from three green microalgae. The highest lipid content was found as 27.3 ± 2.6 % of dry weight for Micractinium sp. ME05 at lyophilization and ultrasonnication assisted method with vi Soxhlet method. Biodiesel productivity of Micractinium sp. ME05 was 89 ± 3.1 %. Also, protein concentrations, carbohydrate percentages and chlorophyll contents of Micractinium sp. ME05 were 56.48 ±2.99 %, 15.57 ± 0.79 %, and 114 ± 12.6 μg/mL, respectively. The present work also comprised genetic manupilation of Chlamydomonas reinhardtii 137- by electroporation and the lipid contents of mutants were determined by using high throughput fluorescence technique. As a conclusion, present study is the first report demonstrating biodiesel production from thermo-resistant green microalgae isolated from Central Anatolia, Haymana, Ankara.

Subject Keywords

Microalgae., Algal biofuels., Biomass energy., Microbial fuel cells.

URI

http://etd.lib.metu.edu.tr/upload/12618527/index.pdf
https://hdl.handle.net/11511/24470

Collections

Graduate School of Natural and Applied Sciences, Thesis

Suggestions

OpenMETU
Core

Mathematical modeling of FBC'a co-fired with lignite and biomass Moralı, Ekrem Mehmet; Selçuk, Nevin; Department of Chemical Engineering (2007) Increasing environmental legislations on pollutant emissions originated from fossil fuel combustion and intention of increasing the life of existing fossil fuels give rise to the use of renewable sources. Biomass at this juncture, with its renewable nature and lower pollutant emission levels becomes an attractive energy resource. However, only seasonal availability of biomass and operation problems caused by high alkaline content of biomass ash restrict its combustion alone. These problems can be overcome b...
MODELING OF BIPOLAR PLATES FOR PROTON EXCHANGE MEMBRANE FUEL CELLS Ekiz, Ahmet; Camci, Talha; Turkmen, Ibrahim; SANKIR, MEHMET; USLU, SITKI; Baker, Derek Keıth; Agar, Ertan (2011-09-01) Fuel cell technology is one of the most economic and efficient ways to utilize hydrogen energy. Various types of fuel cells are present regarding the fuel type and amount of power produced. Among these, proton exchange membrane fuel cells (PEMFCs) are very promising. In this work, a 2D proton exchange membrane fuel cell unit cell was modeled using Comsol Multiphysics software. Cell section was taken parallel to flow direction. Obstacles with various geometries were placed in the flow channel in order to for...
Biohydrogen production in an outdoor panel photobioreactor on dark fermentation effluent of molasses Avcioglu, Sevler Gokce; Ozgur, Ebru; Eroglu, Inci; Yucel, Meral; Gündüz, Ufuk (2011-08-01) Hydrogen is regarded as an ideal energy carrier if it is produced from renewable resources such as biomass. Sequential operation of dark and photofermentation allows a highly efficient production of hydrogen from biomass, as maximal conversion of the energy in the carbohydrates to hydrogen can be achieved. In this study photofermentative hydrogen production was carried out in a solar panel photobioreactor by Rhodobacter capsulatus wild type (DSM 1710) and Rhodobacter capsulatus hup(-) (YO3) strain on the mo...
Numerical investigation of a stand alone solar hydrogen energy system effects of PEFC degradation Ender, Ozden; Tarı, İlker (null; 2015-08-12) An existing stand-alone solar energy system producing hydrogen for energy storage is numerically investigated focusing on the degradation of Polymer Electrolyte Fuel Cell (PEFC) and its effects on the overall performance of the system. The system consists of Photovoltaic (PV) panels, polymer electrolyte based electrolyzers, H2 and O2 storage tanks and a commercial PEFC stack. A PEFC is numerically investigated both as new and as degraded (for about two years). Using a variety of observed degradation pattern...
GIS-based site selection methodology for hybrid renewable energy systems: A case study from western Turkey Aydin, Nazli Yonca; Kentel Erdoğan, Elçin; Duzgun, H. Sebnem (Elsevier BV, 2013-06-01) Renewable energy sources are presently being considered as alternatives to fossil fuels, because they are perpetual, environmentally friendly, and release negligible amounts of greenhouse gases to the atmosphere while producing energy. A disadvantage of renewable energy systems, however, is that continuous energy generation is not possible by using only one type of renewable energy system, since renewable energy resources depend on climate and weather conditions. Two or more renewable energy systems can be ...

Citation Formats

M. Onay, “Bio-fuel production from microalgae,” Ph.D. - Doctoral Program, Middle East Technical University, 2015.