Comparison of optical measurements for better NI tracking in bacteria

Sungur, Sıla
Metal pollution is a common problem in industrial areas. Microorganisms, as well as macroorganisms are affected from metal pollution. In order to cope with this problem, microorganisms develop resistance mechanisms. Traditional microbiological techniques alone are not sufficient in resistance studies. Additional data acquisition by using other measurement techniques and devices are also required. However, there are differences in sentitivities of the measurement devices. A given device may work under a set of conditions and concentrations but may not be suitable when the conditions and concentrations change. Therefore, testing the efficiency of measurement device that is suitable to produce meaningful data is essential. In this study, the possibility and feasibility of using spectroscopy based measurements to quantitate Ni in the spent media of bacterial cultures were explored. The UV-Vis Spectroscope and ATR-FTIR spectroscope were the two optical devices that were compared in this study. Two strains of Microbacterium oxydans, another undefined Microbacterium freshwater isolate, and E. coli were grown at their corresponding minimum inhibitory concentrations of Ni. Then the Ni concentrations left in the culture supernatants following the removal of bacteria were measured by UV-Vis and ATR-FTIR spectroscopes. The data obtained from the two devices were compared. The results indicated that ATR-FTIR spectroscopy had a higher sensitivity and the band intensities provided reasonable approximation for better estimations of Ni concentrations. The data were discussed in terms of detection of metals while the metal sorption capacities of bacteria being evaluated.


Investigation of the Water Hammer Problems in Water-Wind Hybrid Systems
Dincer, Ali Ersin; Bozkuş, Zafer (2015-07-01)
Water hammer is an undesirable event, caused by sudden flow changes in confined pipe systems. When it occurs, its consequences can be very costly and even sometimes deadly. In general, it may be encountered in the penstocks of hydropower plants, water transmission lines, water networks, etc. Therefore, the operation guidelines of the hydropower plants should be defined correctly and if necessary, preventive measures should be considered. In the present study, water hammer problems in pumped-storage hydropow...
Use of laboratory-grown bacterial alginate in copper removal
Moral, C. Kivilcimdan; Doğan, Özdemir; Sanin, Faika Dilek (IWA Publishing, 2012-01-01)
Industrial production leads to toxic heavy metal pollution in water bodies. Copper is one of the examples that requires removal from effluents before being discharged. It is difficult and sometimes very expensive to remove toxic heavy metals by conventional treatment techniques. This study aims to remove copper by the use of bacterial alginate as a non-conventional technique. Bacterial alginates (natural polymers composed of mannuronic and guluronic acid monomers) were synthesized by Azotobacter vinelandii ...
Air Quality of the Black Sea Region: Local and Long Range Transported Pollutants
Alagha, Omar; Tuncel, Süleyman Gürdal; Tosun, Suat (2003-02-01)
Catalysis has manifested its role as a fundamental tool in pollution prevention. While catalysis has long been utilized in increasing efficiency, yield, and selectivity, it is now also recognized as accomplishing a wide range of green chemistry goals. Air pollutants produced by industrial and man-made emissions possesses a unique property that it could affect regional and global receptors. Measuring and defining the levels of pollutants and its sources whether it is local, regional or global should precede ...
Quick Discrimination of Heavy Metal Resistant Bacterial Populations Using Infrared Spectroscopy Coupled with Chemometrics
Gurbanov, Rafig; Ozek, Nihal Simsek; Gözen, Ayşe Gül; Severcan, Feride (2015-10-06)
Lead and cadmium are frequently encountered heavy metals in industrially polluted areas. Many heavy metal resistant bacterial strains have a high biosorption capacity and thus are good candidates for the removal of toxic metals from the environment. However, as of yet there is no accurate method for discrimination of highly adaptive bacterial strains among the populations present in a given habitat. In this study, we aimed to find distinguishing molecular features of lead and cadmium resistant bacteria usin...
Environmental Applications of Natural Zeolites
Kazemian, Hossein; Gedik, Kadir; İmamoğlu, İpek (2012-01-01)
Environmental pollution particularly at very low concentrations is very difficult to remove from contaminated media. Adsorption by means of relatively inexpensive natural adsorbents such as zeolites can be considered as a cost effective alternative treatment of such contaminated streams. The unique chemical and structural characteristics of natural zeolites made them potential materials for a multitude of environmental applications where materials are needed for effective binding, adsorbing, and filtering. ...
Citation Formats
S. Sungur, “Comparison of optical measurements for better NI tracking in bacteria,” M.S. - Master of Science, Middle East Technical University, 2015.