Hippocampal expression of selected cytochrome P450 isoforms during spatial learning in rats

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2016
Gjota Ergin, Sena
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Despite very extensive studies on the molecular mechanisms of memory formation, it is still little known about the molecular correlates of individual variation in the learning skills within a random population of young normal subjects. The aim of the present study was to investigate the expression of different Cytochrome P450 (CYP) isoforms in the rat hippocampus at different stages of place learning in young Sprague-Dawley rats classified as “good” or “poor” learners. Up-to-date, it is little known about CYP expression and functions in the brain. On the other hand, these enzymes are known to be related to the metabolism of steroids, fatty acids (i.e. arachidonic acid) as well as retinoic acid, the substances important for neural functions. To address this issue, rats were trained in the partially baited 12-arm radial maze (RAM) allowing for the evaluation of both short- and long-term spatial memory. The enzyme levels were determined by Western Blot technique at early, intermediary and late stage of the task acquisition (day 4, day 8 and after reaching a performance criterion of 83% correct responses). Obtained results demonstrated hippocampal expression of CYP2E1, CYP2D1 and CYP7A1 but only CYP2E1 levels negatively correlated with the frequency of both, working and reference memory errors. Interestingly, CYP2E1 levels progressively increased in the course of learning. The CYP7B1- and CYP2B1/2 immunoreactivity was not found in the examined tissue samples. All together, these findings indicate the differential expression and differential effects of the members of CYP family in the rat hippocampus.
Hippocampus (Brain)., Cytochrome P-450., Western immunoblotting., Spatial behavior in animals., Rats as laboratory animals.
http://etd.lib.metu.edu.tr/upload/12619692/index.pdf
https://hdl.handle.net/11511/25396
Graduate School of Natural and Applied Sciences, Thesis

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Citation Formats
S. Gjota Ergin, “Hippocampal expression of selected cytochrome P450 isoforms during spatial learning in rats,” M.S. - Master of Science, Middle East Technical University, 2016.
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