Determination of phenolic and flavoniod compounds in rehum ribes

Fazeli, Sepideh
This study involves the investigation of antioxidant phenolic and flavonoid compounds in Rheum ribes L. There is a continuous mounting pressure, in the scientific world, to discover new and potent antioxidants, to combat the free radicals. Among the antioxidant compounds, the phenolics and flavonoids comprise the largest class, and thus hold an important place in biochemical studies. Rheum ribes belong to the family of Polygonaceae in the genus Rheum, which is consumed as a medicinal plant. Since this plant is a source of one of the most important crude drugs in the Middle East (Kashiwada et al., 1988), its antioxidative properties were also worthwhile to be investigated as well as its phenolic constituents. In this study, the crude extract of the roots of Rheum ribes subjected to solvent-solvent fractionation. and each of the fractions were then subjected to the DPPH , total phenolic content and total flavonoids tests. Ethyl acetate fraction exhibited the highest antioxidant capacity, total phenolics and flavonoids compounds. Further analysis by RP-HPLC showed that all fractions contained different number of phenolic compounds and gallic acid, p-coumaric acid and quercetin were present according to the co-injection in RP-HPLC in ethyl acetate extract.
Citation Formats
S. Fazeli, “Determination of phenolic and flavoniod compounds in rehum ribes,” M.S. - Master of Science, Middle East Technical University, 2016.