Biochemical and genetic assessment of high value lipid production from local thermo-resistant green microalgae strain

Download

index.pdf

Date

2017

Author

Akın, Dilan

Metadata

Show full item record

Item Usage Stats

188
views

55
downloads

Green microalgae are a rich source of lipids available for animal and human consumption and for an important form of bioenergy; biodiesel. Selection and genetic engineering of a microalgal strain for sustainable and low-cost biotechnological processes is of utmost importance. In this study, a novel thermo-resistant microalgae strain; Scenedesmus sp. METUNERGY1402 (ME02) was assessed for high-value lipid production via biochemical and genetic approaches. An optimized growth medium for efficient growth of Scenedesmus sp. ME02 has been developed. In terms of biochemical and physiological aspects of microalgae, temperature is critical parameter in cultivation of microalgae and temperature control vi presents an additional cost in outdoor bioreactors. Previously, Scenedesmus sp. ME02 was reported to survive at 25-50°C. This study showed that this strain could also withstand temperatures as low as 10°C. The effects of diurnal temperature fluctuation on biomass and lipid productivities of Scenedesmus sp. ME02 were examined and compared to other temperature regimes. Fatty acid methyl ester (FAME) profiles of Scenedesmus sp. ME02 at different temperatures was also investigated in this study. Significant changes were observed particularly in economically important FAME profiles (palmitic, stearic, oleic, linoleic and linolenic acids). In order to dissect the genetic basis of the changes in fatty acid composition, fatty acid desaturase-2 (fad2) gene was identified and partially cloned from Scenedesmus sp. ME02. Finally, Scenedesmus sp. ME02 was successfully and stably transformed via genetic engineering technology. This is the first report of such a laboratory set-up implemented in batch cultures that can provide an insight for outdoor cultivation under extreme temperatures.

Subject Keywords

Microalgae., Scenedesmus., Biodiesel fuels., Biochemical genetics.

URI

http://etd.lib.metu.edu.tr/upload/12620746/index.pdf
https://hdl.handle.net/11511/26236

Collections

Graduate School of Natural and Applied Sciences, Thesis

Suggestions

OpenMETU
Core

Microbial Control of Milk and Milk Products Guzel, Mustafa; Soyer, Yeşim (2017-01-01) Milk has been the one of the main nutrient sources of human diet for centuries. Microbial studies on milk date back to the seventeenth century, when Kircher used a microscope, and observed the minute worms in milk. Two centuries later, in the1850s, Pasteur proved that the spoilage of milk resulting the sour taste was caused by microorganisms. Pasteur's discoveries on the effect of heat on undesirable microorganisms in beer and wine opened a new era in food science. Therefore, the process was named "pasteuri...
Biochemically based modeling study of Antarctic krill Euphausia superba growth and development Fach Salihoğlu, Bettina Andrea; Wolf-Gladrow, Dieter; Bathmann, Ulrich (2008-01-01) A biochemical model of Antarctic krill Euphausia superba was developed to investigate the physiological mechanisms which enable krill to survive winter, when food is scarce. In this modeling approach data sets on the biochemical composition of krill and its food sources are combined into a model that takes food quality into account rather than just food availability during different seasons. Krill is defined in terms of protein, neutral lipid, polar lipid, carbohydrate, chitin, and ash content, and the mode...
Phenolic compounds, carotenoids, and antioxidant capacities of a thermo-tolerant Scenedesmus sp. (Chlorophyta) extracted with different solvents Bulut, Onur; Akin, Dilan; Sonmez, Cagla; Oktem, Aysegul; Yucel, Meral; Öktem, Hüseyin Avni (Springer Science and Business Media LLC, 2019-06-01) The human body can fight against the adverse effects of chronic exposure to environmental pollutants and stress by consumption of a diet rich in antioxidants. Although fruits and vegetables are the predominant sources, alternative sources of antioxidants such as microalgae are also being explored. Here, we investigate the antioxidant capacity, total phenolic, flavonoid, and carotenoid contents of novel thermo-resistant green microalga Scenedesmus sp. ME02. This strain has previously been shown to have a hig...
IMMUNE RESPONSES AGAINST CHIMERIC DNA AND PROTEIN VACCINES COMPOSED OF PLPEN-OMPH AND PLPEC-OMPH FROM PASTEURELLA MULTOCIDA A:3 IN MICE Okay, Sezer; Ozcengiz, Erkan; Özcengiz, Gülay (Akademiai Kiado Zrt., 2012-12-01) Pasteurella multocida is a pathogenic bacterium causing many diseases that are of significant economic importance to livestock industries. Outer membrane protein H (ompH) gene and two fragments of Pasteurella lipoprotein E (plpE) gene, namely plpEN and plpEC, were cloned from P. multocida A:3. Three DNA vaccine formulations, namely pCMV-ompH, pCMV-plpEN-ompH and pCMV-plpEC-ompH and two protein-based prototype vaccines, alum adjuvanted PlpEN-OmpH and PlpEC-OmpH, were generated. Antibody levels were induced i...
Microencapsulation of wheat germ oil Yazicioglu, Basak; Şahin, Serpil; Şümnü, Servet Gülüm (2015-06-01) Wheat germ oil (WGO) is beneficial for health since it is a rich source of omega-3, omega-6 and tocopherol. However, as it contains polyunsaturated fatty acids, it is prone to oxidation. The aim of this study was to encapsulate wheat germ oil and determine the effects of core to coating ratio, coating materials ratio and ultrasonication time on particle size distribution of emulsions and encapsulation efficiency (EE) and surface morphology of capsules. Maltodextrin (MD) and whey protein concentrate (WPC) at...

Citation Formats

D. Akın, “Biochemical and genetic assessment of high value lipid production from local thermo-resistant green microalgae strain,” M.S. - Master of Science, Middle East Technical University, 2017.