IMMUNE RESPONSES AGAINST CHIMERIC DNA AND PROTEIN VACCINES COMPOSED OF PLPEN-OMPH AND PLPEC-OMPH FROM PASTEURELLA MULTOCIDA A:3 IN MICE

Date

2012-12-01

Author

Okay, Sezer
Ozcengiz, Erkan
Özcengiz, Gülay

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Pasteurella multocida is a pathogenic bacterium causing many diseases that are of significant economic importance to livestock industries. Outer membrane protein H (ompH) gene and two fragments of Pasteurella lipoprotein E (plpE) gene, namely plpEN and plpEC, were cloned from P. multocida A:3. Three DNA vaccine formulations, namely pCMV-ompH, pCMV-plpEN-ompH and pCMV-plpEC-ompH and two protein-based prototype vaccines, alum adjuvanted PlpEN-OmpH and PlpEC-OmpH, were generated. Antibody levels were induced in mice vaccinated with chimeric DNA or protein vaccines. A significant (p<0.05) increase in serum IFN-gamma titer was obtained by vaccination with 100 mu g of pCMV-ompH, pCMV-plpEC-ompH and PlpEC-OmpH. DNA vaccines did not provide protection upon intraperitoneal challenge with 10 LD50 of live P. multocida A: 3. However, 40% protection was conferred by 100 mu g of PlpEC-OmpH which was not statistically significant. These results showed that plpEN-ompH and plpEC-ompH chimeric DNA vaccines and alum adjuvanted PlpEN-OmpH or PlpEC-OmpH protein vaccines were immunogenic but not protective against P. multocida A: 3 in mice. Prime-boost strategies, i.e. priming with DNA vaccines and boost with protein formulations or different adjuvants can be utilized to obtain significant protection.

Subject Keywords

General Immunology and Microbiology, General Medicine

URI

https://hdl.handle.net/11511/40759

Journal

ACTA MICROBIOLOGICA ET IMMUNOLOGICA HUNGARICA

DOI

https://doi.org/10.1556/amicr.59.2012.4.6

Collections

Department of Biology, Article

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Citation Formats

S. Okay, E. Ozcengiz, and G. Özcengiz, “IMMUNE RESPONSES AGAINST CHIMERIC DNA AND PROTEIN VACCINES COMPOSED OF PLPEN-OMPH AND PLPEC-OMPH FROM PASTEURELLA MULTOCIDA A:3 IN MICE,” ACTA MICROBIOLOGICA ET IMMUNOLOGICA HUNGARICA, pp. 485–498, 2012, Accessed: 00, 2020. [Online]. Available: https://hdl.handle.net/11511/40759.