IMMUNE RESPONSES AGAINST CHIMERIC DNA AND PROTEIN VACCINES COMPOSED OF PLPEN-OMPH AND PLPEC-OMPH FROM PASTEURELLA MULTOCIDA A:3 IN MICE

Date

2012-12-01

Author

Okay, Sezer
Ozcengiz, Erkan
Özcengiz, Gülay

Metadata

Show full item record

This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

Item Usage Stats

171
views

0
downloads

Pasteurella multocida is a pathogenic bacterium causing many diseases that are of significant economic importance to livestock industries. Outer membrane protein H (ompH) gene and two fragments of Pasteurella lipoprotein E (plpE) gene, namely plpEN and plpEC, were cloned from P. multocida A:3. Three DNA vaccine formulations, namely pCMV-ompH, pCMV-plpEN-ompH and pCMV-plpEC-ompH and two protein-based prototype vaccines, alum adjuvanted PlpEN-OmpH and PlpEC-OmpH, were generated. Antibody levels were induced in mice vaccinated with chimeric DNA or protein vaccines. A significant (p<0.05) increase in serum IFN-gamma titer was obtained by vaccination with 100 mu g of pCMV-ompH, pCMV-plpEC-ompH and PlpEC-OmpH. DNA vaccines did not provide protection upon intraperitoneal challenge with 10 LD50 of live P. multocida A: 3. However, 40% protection was conferred by 100 mu g of PlpEC-OmpH which was not statistically significant. These results showed that plpEN-ompH and plpEC-ompH chimeric DNA vaccines and alum adjuvanted PlpEN-OmpH or PlpEC-OmpH protein vaccines were immunogenic but not protective against P. multocida A: 3 in mice. Prime-boost strategies, i.e. priming with DNA vaccines and boost with protein formulations or different adjuvants can be utilized to obtain significant protection.

Subject Keywords

General Immunology and Microbiology, General Medicine

URI

https://hdl.handle.net/11511/40759

Journal

ACTA MICROBIOLOGICA ET IMMUNOLOGICA HUNGARICA

DOI

https://doi.org/10.1556/amicr.59.2012.4.6

Collections

Department of Biology, Article

Suggestions

OpenMETU
Core

Investigation of the role of glutathione S-transferase isozymes in pyrethroid resistance of Helicoverpa armigera in Turkey Konus, Metin; Ugurlu, Sakine; İşcan, Mesude (2009-09-13) Helicoverpa armigera, cotton bollworm, is one of the insects causing severe yield loss of economically important crops including cotton, legumes and vegetables. It has developed resistance against the pyrethroid insecticides that have been introduced into market. Resistance against pyrethroids have been reported in H. armigera populations at various parts of the world, however, except 1-chloro-2,4-dinitrobenzene substrate (CDNB), almost no any other substrates used for analysis of the role of glutathione S-...
Targeted disruption of homoserine dehydrogenase gene in Streptomyces clavuligerus and its effects on cephamycin C production Çaydaşı (Koca), Ayşe; Özcengiz, Gülay; Department of Biology (2006) The members of the genus Streptomyces are well-known for their capacity to synthesize a vast repertoire of secondary metabolites, including many useful antibiotics and proteins. Streptomyces clavuligerus is the producer of the medically important β-lactam antibiotics such as cephamycin C and the potent β-lactamase inhibitor clavulanic acid. The aspartate pathway of S. clavuligerus is an important primary metabolic pathway providing substrates for β-lactam synthesis. This pathway uses L-aspartic acid as the ...
Injury recovery of foodborne pathogens in high hydrostatic pressure treated milk during storage Bozoglu, Faruk; Alpas, Hami; Kaletunç, Gönül (Oxford University Press (OUP), 2004-04-09) Bacteria are expected to be injured or killed by high hydrostatic pressure (HHP). This depends on pressure levels, species and strain of the microorganism and subsequent storage. Injured bacteria may be repaired which could affect the microbiological quality of foodstuffs with an important safety consideration especially in low acid food products. In this Study two Gram-positive (Listeria monocytogenes CA and Staphylococcus aureus 485) and two Gram-negative (Escherichia coli O157:H7 933 and Salmonella enter...
Cloning of chitinase a gene (chiA) from serratia marcescens Bn10 and its expression in coleoptera-specific bacillus thuringiensis Okay, Sezer; Özcengiz, Gülay; Department of Biology (2005) Chitinases have been shown to be potential agents for biological control of the plant diseases caused by various phytopathogenic fungi and insect pests, because fungal cell walls and insect exoskeletons contain chitin as a major structural component. Chitinase has also been found to increase the efficacy and potency of Bacillus thuringiensis crystal (Cry) proteins toxic to larvae of insect pests. The reason of this synergy is the presence of chitin in the structure of the outer membrane of larval midgut. In...
Salmonella enterica Serotype 4,5,12:i:-, an Emerging Salmonella Serotype That Represents Multiple Distinct Clones Soyer, Yeşim; Davis, M. A.; Maurer, J.; McDonough, P. L.; Schoonmaker-Bopp, D. J.; Dumas, N. B.; Root, T.; Warnick, L. D.; Groehn, Y. T.; Wiedmann, M. (American Society for Microbiology, 2009-11-01) The prevalence, among human clinical cases, of Salmonella enterica serotype 4,5,12: i:-, a serotype antigenically similar to Salmonella enterica serotype Typhimurium but lacking second-phase flagellar antigens, has increased considerably over the last 10 years. To probe the evolution and ecology of this emerging serotype, we characterized 190 Salmonella isolates initially classified as Salmonella serotypes 4,5,12: i:- (n = 90) and Typhimurium (n = 100) and obtained from various sources in the United States ...

Citation Formats

S. Okay, E. Ozcengiz, and G. Özcengiz, “IMMUNE RESPONSES AGAINST CHIMERIC DNA AND PROTEIN VACCINES COMPOSED OF PLPEN-OMPH AND PLPEC-OMPH FROM PASTEURELLA MULTOCIDA A:3 IN MICE,” ACTA MICROBIOLOGICA ET IMMUNOLOGICA HUNGARICA, pp. 485–498, 2012, Accessed: 00, 2020. [Online]. Available: https://hdl.handle.net/11511/40759.