Research of phytochemical AMPK activators against obesity and development of dietary supplements

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2016

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Kümbet, Yeşim

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A fit and healthy life style has been more and more accepted throughout the world. In that sense, an increasing interest on phytochemicals has been recognized as developing dietary supplements against obesity. Many related products in the markets were hazardous and their uses have caused irremediable results, even in death. For this very reason, this study was intended scientifically to develop a true dietary supplement for obesity. In the energy metabolism, AMP-activated protein kinase (AMPK) has a significant role over the pathway enzymes. AMPK has also been implicated in the central regulation of food intake and energy expenditure. Thus, appropriate phytochemicals as the activators of AMPK might make the recovery possible from obesity. In this study, the selected plants were Citrus limon (as peel and juice), Berberis integerrima and Taraxacum bessarabicum which have been used in folk medicine for appetite suppression. Extracted and fractionated plants examined for their phenolic contents as well as for their radical scavenging capacities through the methods of DPPH and ABTS. The extracts showing high radical scavenging capacity were carried out for the treatments on adipocyte-like (differentiated 3T3-L1) cells. The AMPK activation in the differentiated 3T3-L1 cells was monitored via phosphorylation assay using fluorescence detected ELISA. Moreover, the AMPK protein expression was observed by qRT-PCR. The ethyl acetate fractionated extracts of C. limon peel, B. integerrima and T. bessarabicum were found to be effective AMPK activators at concentrations of as low as 10 µg/mL. Consequently, they can be considered as suitable extracts for the development of fat reducing dietary supplements.

Subject Keywords

Obesity., Antioxidants., Phytochemicals., Dietary supplements., Activation (Chemistry).

URI

http://etd.lib.metu.edu.tr/upload/12620715/index.pdf
https://hdl.handle.net/11511/26298

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Graduate School of Natural and Applied Sciences, Thesis

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Citation Formats

Y. Kümbet, “Research of phytochemical AMPK activators against obesity and development of dietary supplements,” Ph.D. - Doctoral Program, Middle East Technical University, 2016.