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Immobilization of invertase in pyrrole capped polyazotetrahydrofuran/polypyrrole and polytetrahydrofuran-block-polystyrene/polypyrrole conducting graft polymer matrices
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093320.pdf
Date
2000
Author
Erginer, Reyhan
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https://hdl.handle.net/11511/2640
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Graduate School of Natural and Applied Sciences, Thesis
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Immobilization of invertase in conducting polypyrrole polytetrahydrofuran graft polymer matrices
Kizilyar, N; Akbulut, Ural; Toppare, Levent Kamil; Ozden, MY; Yagci, Y (1999-06-24)
Four different polypyrrole/polytetrahydrofuran/invertase electrodes were constructed by the entrapment of invertase in conducting polymer matrices via electropolymerization. Immobilization of the enzyme was achieved by the application of a 1.1 V constant potential to a platinum electrode for 30 min in a solution containing 0.01 M pyrrole, 0.4 mg/ml invertase and 0.4 mg/ml sodium dodecyl sulphate. Performance of each electrode was optimized by examining the effects of pH and temperature on the properties of ...
Immobilization of invertase in conducting polypyrrole/PMMA-co-PMTM graft copolymers
Yildiz, HB; Kiralp, S; Toppare, Levent Kamil; Yagci, Y (Wiley, 2005-04-15)
In this study, invertase was immobilized in copolymer electrodes constructed. Three different types of polymethyl methacrylate-co-polymethyl thienyl methacrylate matrices were used to obtain copolymers that were characterized by FT-IR spectroscopy. Immobilization of enzymes was carried out by the entrapment of the enzyme in conducting polymer matrices during electrochemical polymerization of pyrrole through thiophene moieties of polymers. Immobilization of the enzyme was achieved by application of 1.0 V con...
Immobilization of invertase in conducting polymer matrices
Selampinar, F; Akbulut, Ural; Ozden, MY; Toppare, Levent Kamil (1997-09-01)
This paper reports a novel approach in the electrode immobilization of an enzyme, invertase, by electrochemical polymerization of pyrrole in the presence of enzyme. The polypyrrole/invertase and polyamide/polypyrrole/invertase electrodes were constructed by the entrapment of enzyme in conducting matrices during electrochemical polymerization of pyrrole. This study involves the preparation and characterization of polypyrrole/invertase and polyamide/polypyrrole/invertase electrodes under conditions compatible...
Immobilization of catalase in poly(isopropylacrylamide-co-hydroxyethylmethacrylate) thermally reversible hydrogels
Arica, MY; Öktem, Hüseyin Avni; Oktem, Z; Tuncel, SA (1999-09-01)
Catalase was entrapped in thermally reversible poly (isopropylacrylamide-co-hydroxyethyl-methacrylate) (pNIPAM/HEMA) copolymer hydrogels. The thermoresponsive hydrogels, in cylindIl-rical geometry, were prepared in an aqueous buffer by redox polymerization. It was observed that upon entrapment, the activity retention of catalase was decreased between 47 and 14%, and that increasing the catalase loading of hydrogel adversely affected the activity. The kinetic behaviour of the entrapped enzyme was investigate...
Immobilization of invertase in conducting copolymers of 3-methylthienyl methacrylate
Çırpan, Ali; Toppare, Levent Kamil; Yagci, Y. (Elsevier BV, 2003-4)
Immobilization of invertase in conducting copolymer matrices of 3-methylthienyl methacrylate with pyrrole and thiophene was achieved by constant potential electrolysis using sodium dodecyl sulfate (SDS) as the supporting electrolyte. Polythiophene (PTh) was also used in entrapment process for comparison. Kinetic parameters, Michaelis-Menten constant, Km, and the maximum reaction rate, Vmax, were investigated. Operational stability and temperature optimization of the enzyme electrodes were also examined.
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R. Erginer, “Immobilization of invertase in pyrrole capped polyazotetrahydrofuran/polypyrrole and polytetrahydrofuran-block-polystyrene/polypyrrole conducting graft polymer matrices,” Middle East Technical University, 2000.