Immobilization of invertase in conducting copolymers of 3-methylthienyl methacrylate

Immobilization of invertase in conducting copolymer matrices of 3-methylthienyl methacrylate with pyrrole and thiophene was achieved by constant potential electrolysis using sodium dodecyl sulfate (SDS) as the supporting electrolyte. Polythiophene (PTh) was also used in entrapment process for comparison. Kinetic parameters, Michaelis-Menten constant, Km, and the maximum reaction rate, Vmax, were investigated. Operational stability and temperature optimization of the enzyme electrodes were also examined.


Synthesis and characterization of conducting copolymers of thiophene functionalized monomers and polymers
Papila, Özge; Toppare, Levent Kamil; Department of Chemistry (2003)
Synthesis and characterization of conducting copolymers were achieved by using thiophene end-funtionalized TEMPO initiated polystyrene (PS), decanoic acid dithiophen-3yl-me1hyl ester (DTME) and octanoic acid thiophen-3yl-methyl ester (OTME). Redox behavior of polymer and monomers were done by Cyclic Voltammetry (CV). Structural and characterization of samples were carried out by XB., 13C Nuclear Magnetic Resonance (NMR) and Fourier Transform Infrared Spectroscopy (FTIR). Thermal analyses of resultant conduc...
Epoxidation reactions of small alkenes on catalytic surfaces
Kurnaz, Emine; Önal, Işık; Department of Chemical Engineering (2011)
Propylene epoxidation reaction was investigated on catalytic surfaces of chlorinated copper(I) oxide and ruthenium(IV) oxide using periodic density functional theory (DFT). Cu2O(001) and (110) surface of RuO2 was selected to generate chlorinated surfaces to be used in the study. Besides epoxidation, other reactions that compete with epoxidation were also studied such as formations of allyl-radical, acrolein, acetone on chlorinated Cu2O(001) and formations of propionaldehyde, allyl-radical and acetone on chl...
Carbas, Buket Bezgin; Odabas, Serhat; Turksoy, Figen; Tanyeli, Cihangir (2016-03-01)
Poly-1,1,2,2-tetrakis(4-9H-carbazol-9-yl)phenyl)ethene P(TCP) was successfully synthesized by electrochemical oxidation of corresponding monomer, namely, 1,1,2,2-tetrakis(4-9H-carbazol-9-yl)phenyl)ethene (TCP) using dichloromethane as the solvent and tetrabutylammonium hexafluorophosphate (TBAPF(6)) as supporting electrolyte. Spectroelectrochemical properties of P(TCP) were investigated in situ recording the electronic absorption spectra of the polymer film coated on indium-tin oxide (ITO) at various potent...
Thermal analysis of a new thiophene derivative and its copolymer
Aslan, E.; Toppare, Levent Kamil; Hacaloğlu, Jale (2008-06-01)
Thermal characteristics of a new thiophene derivative, 2-(thiophen-3-yl-)ethyl octanoate (OTE), its homopolymer (POTE), and copolymer with thiophene P(OTE-co-Th) were investigated via pyrolysis mass spectrometry. Thermal degradation of the copolymer started by lose of side chains and thiophene involving products evolved almost in the same temperature range where PTh degradation was detected, at slightly higher temperatures than PTh backbone decomposed during the pyrolysis of POTE. The extent of doping and n...
Preparation of biosensors by immobilization of polyphenol oxidase in conducting copolymers and their use in determination of phenolic compounds in red wine
Boyukbayram, A. Efif; Kiralp, Senem; Toppare, Levent Kamil; Yagci, Yusuf (2006-10-01)
Electrochemically produced graft copolymers of thiophene capped polytetrahydofuran (TPTHF1 and TPTHF2) and pyrrole were achieved by constant potential electrolysis using sodium dodecylsulfate (SDS) as the supporting electrolyte. Characterizations were based on Fourier transform infrared spectroscopy (FTIR) and scanning electron microscopy (SEM). Electrical conductivities were measured by the four-probe technique.
Citation Formats
A. Çırpan, L. K. Toppare, and Y. Yagci, “Immobilization of invertase in conducting copolymers of 3-methylthienyl methacrylate,” Bioelectrochemistry, pp. 29–33, 2003, Accessed: 00, 2020. [Online]. Available: