The Effects of sex ratio in estimation of genetic differentiation in populus nigra populations

Date

2017

Author

Yelmen, Burak

Metadata

Show full item record

Item Usage Stats

135
views

0
downloads

Effective population size is an important concept in conservation biology. Biased sex ratio lowers effective population size, consequently causing loss of genetic variation. In this study, available microsatellite DNA marker data of 121 Populus nigra clones originated from 5 geographical regions were analyzed to evaluate genetic diversity of genders and to investigate possible effects of sex ratio on differentiation in these populations in Turkey. There was an abundance of identical genotypes in the dataset. The same genotypes were observed both in males and females that might be suggesting a rare occurrence of deviation from dioecism. Three clusters were estimated in structure analysis with k-means clustering. Significant differentiation was found among clusters. However, they did not correlate with geographical assignment of clones. Also, there was no variation between pre-determined five geographical populations. Overall allelic richness was found to be similar for both genders whereas heterozygosity was higher in males. Both male and female group had private alleles on all studied loci. Although there was no significant variation between genders, combination of four loci showed a slight empirical differentiation. Additionally, a simulation software prototype was developed to see effects of sex ratio on diversity and differentiation in future generations given the available molecular data. Results showed that if biased sex ratio persists, allele loss and fixation might occur in a higher rate, causing a loss of variation and faster differentiation in case of isolation.

Subject Keywords

Plant genetics., Poplar, Plants, Plant population genetics.

URI

http://etd.lib.metu.edu.tr/upload/12621132/index.pdf
https://hdl.handle.net/11511/26604

Collections

Graduate School of Natural and Applied Sciences, Thesis

Suggestions

OpenMETU
Core

Impact of biased sex ratio on the genetic diversity, structure, and differentiation of Populus nigra (European black poplar) Yelmen, Burak; Değirmenci, Funda Ö.; Kaya, Zeki (Canadian Science Publishing, 2020-10-01) Effective population size is a crucial concept of conservation biology. It is reduced by biased sex ratio, consequently causing loss of genetic variation. To evaluate genetic diversity related to gender, and investigate the possible effects of biased sex ratio, we analyzed available microsatellite DNA markers from 120 samples of Populus nigra L. (European black poplar) originating from five geographical regions in Turkey. Using 12 microsatellite markers, we detected 60 clones of the same genotype, out of 12...
Assessment of genetic diversity in picea orientalis (l.) link. in genetic resources by microsatellites Özdilek, Aslı; Kaya, Zeki; Temel, Fatih; Department of Biology (2015) Picea comprises about 40 species in the world. One of these species, oriental spruce (Picea orientalis (L.) Link.) is naturally distributed in northeastern Turkey, mostly in Artvin Province and in a part of Georgia close to the coast of the Black Sea region. The limited distribution of species and increased anthropogenic threats to its genetic resources signify the importance of studying genetic diversity of the species to have better conservation and management programs. Here, we report the first high thro...
Determinants of phytoplankton size structure in warm, shallow lakes Erdoğan, Şeyda; Beklioğlu, Meryem; Litchman, Elena; Miller, Elizabeth T.; Levi, Eti E.; Bucak, Tuba; Tavşanoǧlu, Ülkü Nihan (2021-05-01) Body size is an important trait of any organism, including phytoplankton, because it affects physiological and morphological performance, reproduction, population growth rate and competitive interactions. Understanding how interacting top-down and bottom-up factors influence phytoplankton cell size in different aquatic environments is still a challenge. Structural equation modeling (SEM) is a comprehensive multivariate statistical tool for detecting cause-effect relationship among different variables and th...
Physiological and biochemical screeing of different Turkish Lentil (Lens culinaris M.) cultivars under drought stress condition Gökçay, Derya; Yücel, Ayşe Meral; Baloğlu, Mehmet Cengiz; Department of Biotechnology (2012) Legumes being the most important crops worldwide are limited in terms of adaptability and productivity mainly by the abiotic stresses. In this study, the aim was to understand tolerance mechanisms of lentil cultivars under drought stress by physiological and biochemical analyses. This study was carried out with six Turkish Lentil cultivars (Seyran, Kafkas, Malazgirt, Çağıl, Çiftçi, Özbek) subjected to drought stresses (10% and 15% PEG) and their physiological and biochemical properties were examined to sele...
The Role of Top-Down and Bottom-Up Control for Phytoplankton in a Subtropical Shallow Eutrophic Lake: Evidence Based on Long-Term Monitoring and Modeling Mao, Zhigang; Gu, Xiaohong; Cao, Yong; Zhang, Min; Zeng, Qingfei; Chen, Huihui; Shen, Ruijie; Jeppesen, Erik (Springer Science and Business Media LLC, 2020-02-03) Lake ecosystems are exposed to a range of anthropogenic pressures, particularly eutrophication, and in some cases also stocking and/or overfishing of top-predator fish species, all factors that have implications for the food web structure and which could lead to dominance of nuisance cyanobacteria. Restoration of degraded lakes demands insight into the relative role of top-down for bottom-up regulating forces. While knowledge about these forces in temperate lakes is extensive, comparatively little is known ...

Citation Formats

B. Yelmen, “The Effects of sex ratio in estimation of genetic differentiation in populus nigra populations,” M.S. - Master of Science, Middle East Technical University, 2017.