The Molecular investigation of the effects of simvastatin, a cholesterol reducing drug, on rat sciatic nerve tissue

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2017

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Badem Özgün, Kumsal

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Simvastatin is a lipophilic cholesterol decreasing drug, which belongs to statin group. It is previously known that the long-term usage of this drug cause peripheric neuropathy, mononeuropathy and memory problems. However, the molecular effects of this drug on nerve system remain unclear. In the present study, the simvastatin-induced changes on rat sciatic nerve tissue were investigated at molecular level by Attenuated Total Reflection Fourier Transform Infrared (ATR-FTIR) Spectroscopy. ATR-FTIR spectroscopy allows us rapid and sensitive determination of functional groups belonging to proteins, lipids, carbohydrates and nucleic acids simultaneously. Experimental male adult rats were divided two groups as control (n=10) and simvastatin-treated group (n=10). 50 mg simvastatin/ kg was given to treated group by oral gavage for 1 month. In the FTIR spectra, the shift in peak positions, the change in bandwidths and the intensity/area values of the bands were determined. Simvastatin treatment induces a significant decrease in lipid, nucleic acid, protein and glycogen content. A significant decrease in the lipid/ protein ratio indicates that the decrease in lipid concentration is more than the decrease in protein content. Furthermore an increase in lipid order and a decrease in membrane fluidity were detected. A decrease in the bandwidth of the amide I band and shifting of the position of this band to higher frequency values in treated sciatic nerve indicates structural changes in proteins.

Subject Keywords

Statins (Cardiovascular agents)., Fourier transform infrared spectroscopy.

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http://etd.lib.metu.edu.tr/upload/12621452/index.pdf
https://hdl.handle.net/11511/26811

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Graduate School of Natural and Applied Sciences, Thesis

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Citation Formats

K. Badem Özgün, “The Molecular investigation of the effects of simvastatin, a cholesterol reducing drug, on rat sciatic nerve tissue,” M.S. - Master of Science, Middle East Technical University, 2017.