Show/Hide Menu
Hide/Show Apps
Logout
Türkçe
Türkçe
Search
Search
Login
Login
OpenMETU
OpenMETU
About
About
Open Science Policy
Open Science Policy
Open Access Guideline
Open Access Guideline
Postgraduate Thesis Guideline
Postgraduate Thesis Guideline
Communities & Collections
Communities & Collections
Help
Help
Frequently Asked Questions
Frequently Asked Questions
Guides
Guides
Thesis submission
Thesis submission
MS without thesis term project submission
MS without thesis term project submission
Publication submission with DOI
Publication submission with DOI
Publication submission
Publication submission
Supporting Information
Supporting Information
General Information
General Information
Copyright, Embargo and License
Copyright, Embargo and License
Contact us
Contact us
Conjugative transfer of antibiotic resistance genes from salmonella enterica serovar infantis to escherichia coli
Date
2018
Author
Cesur, Aylin
Metadata
Show full item record
Item Usage Stats
262
views
0
downloads
Cite This
The usage and misusage of antibiotics in poultry, food-producing animals and human diseases have led to transmission of conjugative plasmids carrying antibiotic resistance genes from one microorganism to another, especially to the pathogenic bacteria. Multi-drug resistant Salmonella enterica serovar Infantis, an emerging serotype in poultry, has been spreading all around the world in a decade. Moreover, commensal microorganisms such as commensal Escherichia coli in the gut microbiota, functioning as a reservoir of antimicrobial resistance, of the warm-blooded hosts acquire antibiotic resistance by plasmid from Salmonella enterica serovars. The aim of this study was to identify the conjugative transfer of plasmids including multi-drug resistance genes from Salmonella enterica serovar Infantis to commensal susceptible E. coli in both of phenotypic and genotypic level. Salmonella Infantis isolates with different antibiotic resistance profiles involving streptomycin, sulfisoxazole, ampicillin, trimethoprim/sulfamethoxazole, kanamycin, chloramphenicol, ciprofloxacin, cephalotin, nalidixic acid and tetracycline were selected as donor bacteria (n=10), while susceptible non-pathogenic E. coli isolate was used as a recipient. Applying filter paper mating procedure was used for conjugation. Transconjugant E. coli colonies were screened by phenotypic and genotypic methods. First, yellow colonies representing transconjugant E. coli colonies were determined on the selective XLD agar containing 10 μg/ml tetracycline in phenotypic level. After that, conjugation genes (i.e., traA, traE, traL, traJ, traG, traH, traI, traM, traR, traY, rfaG, rfaI, rfaJ, rfaL and rfbP), and the incompatibility group of plasmids were screened by polymerase chain reaction (PCR) in genotypic level. This study contributes to recent and further studies related to the conjugative transmission of antibiotic resistance genes in Salmonella and E. coli, which is an arising serious problem worldwide.
Subject Keywords
Salmonella.
,
Escherichia coli.
,
Drug resistance in microorganisms.
URI
http://etd.lib.metu.edu.tr/upload/12621781/index.pdf
https://hdl.handle.net/11511/27056
Collections
Graduate School of Natural and Applied Sciences, Thesis
Suggestions
OpenMETU
Core
Characterization of plasmids form multi drug resistance salmonella infantis isolates
Aydın, Sacide Özlem; Soyer, Yeşim; Department of Food Engineering (2018)
The usage and misusage of antibiotics in poultry, food-producing animals and human diseases have led to transmission of conjugative plasmids carrying antibiotic resistance genes from one microorganism to another, especially to the pathogenic bacteria. Multi-drug resistant Salmonella enterica serovar Infantis, an emerging serotype in poultry, has been spreading all around the world in a decade. Moreover, commensal microorganisms such as commensal Escherichia coli in the gut microbiota, functioning as a reser...
In vitro antibiotic release from poly(3-hydroxybutyrate-co-3-hydroxyvalerate) rods
Gursel, I; Yagmurlu, F; Korkusuz, F; Hasırcı, Vasıf Nejat (2002-03-01)
Provision and maintenance of adequate concentrations of antibiotics at infection sites is very important in treating highly resistant infections. For diseases like implant related osteomyelitis (IRO) it is best to provide this locally via implanted drug formulations, as systemic administration of the antibiotic may not be effective due to damaged vasculature. In this study, poly(3-hydroxybutyrate-co-3-hydroxyvalerate) (PHBV) rods containing 7, 14 and 22% (mol) 3-hydroxyvalerate were loaded with sulbactam:ce...
Determination of colistin resistance in Escherichia coli isolates from foods in Turkey, 2011-2015
Güzel, Mustafa; Avşaroğlu, M Dilek; Soyer, Yeşim (2020-05-01)
Antimicrobial resistance of pathogenic microorganisms is an emerging public health concern. Intensive use of antibiotics in food animals might increase antimicrobial resistance in foodborne pathogens. Colistin is a last resort antibiotic for treatment of multidrug resistant (MDR) Gram negative pathogens. The recent antimicrobial resistance studies revealed a mobile antimicrobial resistance gene (mcr) that provides resistance to colistin. Furthermore, the gene has been found in different genera. Therefore, t...
Discovery of non-carbohydrate inhibitors of aminoglycoside-modifying enzymes
Welch, KT; Virga, KG; Whittemore, NA; Özen, Can; Wright, E; Brown, CL; Lee, RE; Serpersu, EH (2005-11-15)
Chemical modification and inactivation of aminoglycosides by many different enzymes expressed in pathogenic bacteria are the main mechanisms of bacterial resistance to these antibiotics. In this work, we designed inhibitors that contain the 1,3-diamine pharmacophore shared by all aminoglycoside antibiotics that contain the 2-deoxystreptamine ring. A discovery library of molecules was prepared by attaching different side chains to both sides of the 1,3-diamine motif. Several of these diamines showed inhibito...
A Study on induction of minicell formation by FtsZ overproducing L-form E. coli
Yılmaz, İsmail Cem; Özcengiz, Gülay; Gürsel, Mayda; Department of Biology (2014)
L-form organisms (or L-phase, L-variants) are cell wall-deficient (CWD) bacteria consisting of protoplasts and spheroplasts that have the ability to grow and divide. The mutant strain LW1655F+ (also known as L-form E. coli) was originally derived from the parental E. coli K-12 parental strain and is known to be deficient in cell wall, periplasmic space, flagella, fimbriae and outer membrane. Minicells are small cell-like structures that do not have genomic DNA and are produced during the logarithmic phase o...
Citation Formats
IEEE
ACM
APA
CHICAGO
MLA
BibTeX
A. Cesur, “Conjugative transfer of antibiotic resistance genes from salmonella enterica serovar infantis to escherichia coli,” M.S. - Master of Science, Middle East Technical University, 2018.