Investigation of the therapeutic effect of sodium butyrate in Caco-2 colon cancer cell line by using ATR-FTIR spectroscopy

Download

index.pdf

Date

2018

Author

Çelik, Buket

Metadata

Show full item record

Item Usage Stats

418
views

119
downloads

Sodium butyrate (NaBt), as one of the HDACi, has been demonstrated that it induces apoptosis, cell cycle arrest, the inhibition of angiogenesis, metastasis and gene expression changes. To date, there are several studies perfomed to investigate its therapeutic effect; however, theexact mechanism at molecular level is not clear yet. Therefore, the current thesis was aimed to clarify the action/theurapeutic potential mechanisms of sodium butyrate in Caco2 colon cancer cell line at molecular level using ATR-FTIR spectroscopy, quantitative spectral and chemometric analyses. 3mM, 6mM and 9mM doses of sodium butyrate were applied to Caco2 cell line line for 12h, 24h and 48 h. According to the results, NaBt treatment caused an increase in the unsaturated lipids, impying elevated lipid peroxiation. Moreover increased saturated lipids, membrane fluidity/dynamics and esters and triacylglycerols were obtained in NaBt-treated groups with respect to the control ones. However, NaBt induced a degradation of proteins and nucleic acids. HCA and PCA results indicated that in all studied doses, there was a clear distinction between studied groups in a time dependent manner. The results of the current study implied that sodium butyrate may have therapeutic potential by structural and functional remodelling of cancer cells.

Subject Keywords

Colon (Anatomy), Sodium butyrate.

URI

http://etd.lib.metu.edu.tr/upload/12621824/index.pdf
https://hdl.handle.net/11511/27131

Collections

Graduate School of Natural and Applied Sciences, Thesis

Suggestions

OpenMETU
Core

Investigation of the molecular pathways involved in intestinal epithelial differentiation Sade Memişoğlu, Aslı; Banerjee, Sreeparna; Department of Biology (2014) The molecular mechanisms of balanced and continuous generation of intestinal epithelial cells, is poorly understood and disruption of this balance may result in neoplastic transformation and malignant growth. Differentiation is regulated by numerous signals, which in turn regulate signaling pathways directing activation or inactivation of certain transcription factors. Perturbations like changes in Ca2+ levels, glucose or amino acid starvation result in an ER stress response, which is also implicated in the...
Investigation of the effect of sodium butyrate on the regulation of cyclooxygenase-2 in colon cancer cell lines Caco-2 and HT-29 Ülgen, Doğukan Hazar; Banerjee, Sreeparna; Department of Biology (2015) Sodium butyrate (NaBt) is a four-carbon short-chain fatty acid histone deacetylase inhibitor (HDACi) that is available in the colon through the commensal microbiota-mediated fermentation of dietary fibers. It is the main source of energy for colonocytes, and is regarded to have tumor suppressive effects, most prominently in colorectal cancer (CRC). Cyclooxygenase-2 (COX-2) is a gene important in the inflammatory response due to its ability to convert arachidonic acid to prostaglandins. Overexpression and ov...
Molecular docking study of fda-approved drugs to inhibit the bacterial ribosome Ateş, Beril; Yüce, Merve; Levitaş, Ozge Kurkcuoglu; Sungur, Fethiye Aylin (Orta Doğu Teknik Üniversitesi Enformatik Enstitüsü; 2022-10) Ribosomes are large macromolecular complexes responsible for cellular protein synthesis. It consists of two subunits; called 30S small and 50S large subunits in bacteria, involving antibiotic binding regions. This mac- romolecular machine is one of the significant targets of conventional antibiotics because protein synthesis can be stopped by targeting functional sites in the ribosome. For instance, several antibiotics target the decoding center responsible for deciphering the genetic code, as well as mRNA ...
Building Dicer1 regulation network of mouse liver hepatocytes Ünsal, Şeyma; Beyge, Mahmut; Aydın Son, Yeşim (2012-06-29) RNA induced gene silencing complex (RISC) has a role in many cellular processes which includes regulation of gene expression, immune response, cell differentiation and embryonic development. Dicer protein is a key regulator of these processes. Dicer1 specifically has central role in maturation of RISC substrate RNA and RISC assembly in mouse. Here the results of a microarray study that investigates the molecular level changes in dicer1 knockout mouse liver hepatocytes is re-analysed and the differentially r...
The Effect of 15-lipoxygenase-1 (15-LOX-1) on angiogenesis in colorectal and prostate cancer Çolakoğlu, Melis; Banerjee, Sreeparna; Department of Biology (2017) 15-lipoxygenase-1 (15-LOX-1) is one of the lipoxygenases (LOX) that are important for the generation of inflammatory bioactive lipids, which then result in the initiation and resolution of inflammation. The enzyme mainly metabolizes the omega-6 fatty acid, linoleic acid, to 13-(S)-HODE. In colorectal carcinoma, the tumor suppressive role of 15-LOX-1 is highlighted with decreased proliferation, induction of apoptosis, reduced motility and invasion. Oppositely, the expression of 15-LOX-1 was reported to have ...

Citation Formats

B. Çelik, “Investigation of the therapeutic effect of sodium butyrate in Caco-2 colon cancer cell line by using ATR-FTIR spectroscopy,” M.S. - Master of Science, Middle East Technical University, 2018.