Investigation of SNX3 in colon cancer

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2019

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Cingöz, Harun

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Sorting Nexin 3 (SNX3) is part of the retromer complex that recycles cargo receptors back to plasma membrane or to Trans Golgi Network. WNT ligand carrier protein Wntless (WLS) is a known SNX3 cargo protein. Our earlier data suggested over expression of SNX3 in colon cancer cells. Considering its importance in receptor recycling, we hypothesized SNX3 to be a potential modulator of cancer related receptors. To begin understanding the role of SNX3, we developed RNAi models of SNX3 in SW480 colon cancer cells and investigated biochemical and phenotypical outcomes of SNX3 knock down. Here we present evidence that SNX3 might regulate several receptors important in colon cancers and decreased SNX3 expression alters motility and migration of colon cancer cells by downregulating important signaling pathways with implications in epithelial to mesenchymal transition.

Subject Keywords

Colon (Anatomy), Protein expression., Cancer cells.

URI

http://etd.lib.metu.edu.tr/upload/12622980/index.pdf
https://hdl.handle.net/11511/27939

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Graduate School of Natural and Applied Sciences, Thesis

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Citation Formats

H. Cingöz, “Investigation of SNX3 in colon cancer,” M.S. - Master of Science, Middle East Technical University, 2019.