PST secretome project, a short cut to effector analysis

Özketen, Ahmet Çağlar
Wheat yellow (stripe) rust is a devastating disease on wheat on the global scale. Emergence of new aggressive races leads swift epidemics unless countermeasures are in place. The causative agent of the disease is an obligate biotrophic fungus, Puccinia striiformis f.sp. tritici (Pst). The identification of the infection mechanisms is the best strategy to act against the disease. For that reason, candidate secreted effector proteins (CSEPs) of Pst races in Turkey was monitored using de novo transcriptome sequencing approach combined with data mining on the inoculated tissues of compatible and incompatible interaction. The small-secreted proteins (SSPs) of the identified differentially expressed genes (DEGs) were generated and characterized via in silico analysis. Comparisons with published reports reveal both unique and common sets of candidate effector for virulence. In order to validate reliability of the data, some of the effector candidates were cloned and studied for their function. Unigene17495 (Pstg10917) targets chloroplasts and suppresses the cell death triggered by INF1 elicitor in planta studies. The thesis includes the microarray profiling of differentially expressed genes during compatible and incompatible interaction. Moreover, the new screening methods for determination of biological functions were studied in the thesis in order to pinpoint flexible strategies for screening effectors.
Citation Formats
A. Ç. Özketen, “PST secretome project, a short cut to effector analysis,” Ph.D. - Doctoral Program, Middle East Technical University, 2019.